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Photosynthesis: Using Light to Make Food

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1 Photosynthesis: Using Light to Make Food
Chapter 7 Photosynthesis: Using Light to Make Food

2 Biology and Society: Green Energy
Wood has historically been the main fuel used to: Cook Warm homes Provide light at night © 2010 Pearson Education, Inc.

3 Figure 7.0 Figure 7.0 Sunlight and leaves

4 Industrialized societies replaced wood with fossil fuels.
To limit the damaging effects of fossil fuels, researchers are investigating the use of biomass (living material) as efficient and renewable energy sources.

5 Fast-growing trees, such as willows:
Can be cut every three years Do not need to be replanted Are a renewable energy source Produce fewer sulfur compounds Reduce erosion Provide habitat for wildlife

6 THE BASICS OF PHOTOSYNTHESIS
Is used by plants, some protists, and some bacteria Transforms light energy into chemical energy Uses carbon dioxide and water as starting materials The chemical energy produced via photosynthesis is stored in the bonds of sugar molecules. Student Misconceptions and Concerns 1. Students may understand the overall chemical relationships between photosynthesis and cellular respiration, but many struggle to understand the use of carbon dioxide in the Calvin cycle. Photosynthesis is much more than gas exchange. 2. Students may not connect the growth in plant mass to the fixation of carbon during the Calvin cycle. It can be difficult for many students to appreciate that molecules in air can contribute significantly to the mass of plants. Teaching Tips 1. When introducing the diverse ways that plants impact our lives, consider challenging your students to come up with a list of products made from plants that they encounter regularly. Perhaps you might only list those encountered in a single day of college life. The list can be surprising and help to build up your “catalog of examples.” 2. Within the living world, there are many examples of adaptations to increase surface area. Some examples are the many folds of the inner mitochondrial membrane, the highly branched surfaces of fish gills and human lungs, and the highly branched system of capillaries in the tissues of our bodies. Consider relating this broad principle seen elsewhere to the extensive folding of the thylakoid membranes. 3. In our world, energy is frequently converted to a usable form in one place and used in another. For example, electricity is generated by power plants, transferred to our homes, and used to run computers, create light, and help us prepare foods. Consider relating this common energy transfer to the two-stage process of photosynthesis. 4. You might wish to discuss the evolution of chloroplasts from photosynthetic prokaryotes if you will not address this subject elsewhere in your course. 5. Students who have not read all of chapter 7 may not realize that glucose is not the direct product of photosynthesis. Although glucose is shown as a product of photosynthesis, a three-carbon sugar is directly produced (G3P). A plant can use G3P to make many types of organic molecules, including glucose. (The authors address the production of G3P under the section “The Calvin Cycle” later in this chapter.) 6. Figure 7.3 is an important visual organizer that notes the key structures and functions of the two stages of photosynthesis. This figure reminds students where water and sunlight are used in the thylakoid membranes to generate oxygen, ATP, and NADPH. The second step, in the stroma, reveals the use of carbon dioxide, ATP, and NADPH to generate carbohydrates.

7 Organisms that use photosynthesis are:
Photosynthetic autotrophs The producers for most ecosystems Student Misconceptions and Concerns 1. Students may understand the overall chemical relationships between photosynthesis and cellular respiration, but many struggle to understand the use of carbon dioxide in the Calvin cycle. Photosynthesis is much more than gas exchange. 2. Students may not connect the growth in plant mass to the fixation of carbon during the Calvin cycle. It can be difficult for many students to appreciate that molecules in air can contribute significantly to the mass of plants. Teaching Tips 1. When introducing the diverse ways that plants impact our lives, consider challenging your students to come up with a list of products made from plants that they encounter regularly. Perhaps you might only list those encountered in a single day of college life. The list can be surprising and help to build up your “catalog of examples.” 2. Within the living world, there are many examples of adaptations to increase surface area. Some examples are the many folds of the inner mitochondrial membrane, the highly branched surfaces of fish gills and human lungs, and the highly branched system of capillaries in the tissues of our bodies. Consider relating this broad principle seen elsewhere to the extensive folding of the thylakoid membranes. 3. In our world, energy is frequently converted to a usable form in one place and used in another. For example, electricity is generated by power plants, transferred to our homes, and used to run computers, create light, and help us prepare foods. Consider relating this common energy transfer to the two-stage process of photosynthesis. 4. You might wish to discuss the evolution of chloroplasts from photosynthetic prokaryotes if you will not address this subject elsewhere in your course. 5. Students who have not read all of chapter 7 may not realize that glucose is not the direct product of photosynthesis. Although glucose is shown as a product of photosynthesis, a three-carbon sugar is directly produced (G3P). A plant can use G3P to make many types of organic molecules, including glucose. (The authors address the production of G3P under the section “The Calvin Cycle” later in this chapter.) 6. Figure 7.3 is an important visual organizer that notes the key structures and functions of the two stages of photosynthesis. This figure reminds students where water and sunlight are used in the thylakoid membranes to generate oxygen, ATP, and NADPH. The second step, in the stroma, reveals the use of carbon dioxide, ATP, and NADPH to generate carbohydrates.

8 Photosynthetic Protists Photosynthetic Bacteria
PHOTOSYNTHETIC AUTOTROPHS Plants (mostly on land) Photosynthetic Protists (aquatic) Photosynthetic Bacteria (aquatic) LM Forest plants Kelp, a large alga Micrograph of cyanobacteria Figure 7.1 Figure 7.1 Photosynthetic autotrophs

9 Plants (mostly on land)
Forest plants Figure 7.1a Figure 7.1a Forest plants

10 Photosynthetic Protists
(aquatic) Kelp, a large alga Figure 7.1b Figure 7.1b Kelp, a large alga

11 Photosynthetic Bacteria
(aquatic) LM Micrograph of cyanobacteria Figure 7.1c Figure 7.1c Cyanobacteria

12 Chloroplasts: Sites of Photosynthesis
Chloroplasts are: The site of photosynthesis Found mostly in the interior cells of leaves Student Misconceptions and Concerns 1. Students may understand the overall chemical relationships between photosynthesis and cellular respiration, but many struggle to understand the use of carbon dioxide in the Calvin cycle. Photosynthesis is much more than gas exchange. 2. Students may not connect the growth in plant mass to the fixation of carbon during the Calvin cycle. It can be difficult for many students to appreciate that molecules in air can contribute significantly to the mass of plants. Teaching Tips 1. When introducing the diverse ways that plants impact our lives, consider challenging your students to come up with a list of products made from plants that they encounter regularly. Perhaps you might only list those encountered in a single day of college life. The list can be surprising and help to build up your “catalog of examples.” 2. Within the living world, there are many examples of adaptations to increase surface area. Some examples are the many folds of the inner mitochondrial membrane, the highly branched surfaces of fish gills and human lungs, and the highly branched system of capillaries in the tissues of our bodies. Consider relating this broad principle seen elsewhere to the extensive folding of the thylakoid membranes. 3. In our world, energy is frequently converted to a usable form in one place and used in another. For example, electricity is generated by power plants, transferred to our homes, and used to run computers, create light, and help us prepare foods. Consider relating this common energy transfer to the two-stage process of photosynthesis. 4. You might wish to discuss the evolution of chloroplasts from photosynthetic prokaryotes if you will not address this subject elsewhere in your course. 5. Students who have not read all of chapter 7 may not realize that glucose is not the direct product of photosynthesis. Although glucose is shown as a product of photosynthesis, a three-carbon sugar is directly produced (G3P). A plant can use G3P to make many types of organic molecules, including glucose. (The authors address the production of G3P under the section “The Calvin Cycle” later in this chapter.) 6. Figure 7.3 is an important visual organizer that notes the key structures and functions of the two stages of photosynthesis. This figure reminds students where water and sunlight are used in the thylakoid membranes to generate oxygen, ATP, and NADPH. The second step, in the stroma, reveals the use of carbon dioxide, ATP, and NADPH to generate carbohydrates.

13 Thylakoids are concentrated in stacks called grana.
Inside chloroplasts are membranous sacs called thylakoids, which are suspended in a thick fluid, called stroma. Thylakoids are concentrated in stacks called grana. The green color of chloroplasts is from chlorophyll, a light-absorbing pigment. Student Misconceptions and Concerns 1. Students may understand the overall chemical relationships between photosynthesis and cellular respiration, but many struggle to understand the use of carbon dioxide in the Calvin cycle. Photosynthesis is much more than gas exchange. 2. Students may not connect the growth in plant mass to the fixation of carbon during the Calvin cycle. It can be difficult for many students to appreciate that molecules in air can contribute significantly to the mass of plants. Teaching Tips 1. When introducing the diverse ways that plants impact our lives, consider challenging your students to come up with a list of products made from plants that they encounter regularly. Perhaps you might only list those encountered in a single day of college life. The list can be surprising and help to build up your “catalog of examples.” 2. Within the living world, there are many examples of adaptations to increase surface area. Some examples are the many folds of the inner mitochondrial membrane, the highly branched surfaces of fish gills and human lungs, and the highly branched system of capillaries in the tissues of our bodies. Consider relating this broad principle seen elsewhere to the extensive folding of the thylakoid membranes. 3. In our world, energy is frequently converted to a usable form in one place and used in another. For example, electricity is generated by power plants, transferred to our homes, and used to run computers, create light, and help us prepare foods. Consider relating this common energy transfer to the two-stage process of photosynthesis. 4. You might wish to discuss the evolution of chloroplasts from photosynthetic prokaryotes if you will not address this subject elsewhere in your course. 5. Students who have not read all of chapter 7 may not realize that glucose is not the direct product of photosynthesis. Although glucose is shown as a product of photosynthesis, a three-carbon sugar is directly produced (G3P). A plant can use G3P to make many types of organic molecules, including glucose. (The authors address the production of G3P under the section “The Calvin Cycle” later in this chapter.) 6. Figure 7.3 is an important visual organizer that notes the key structures and functions of the two stages of photosynthesis. This figure reminds students where water and sunlight are used in the thylakoid membranes to generate oxygen, ATP, and NADPH. The second step, in the stroma, reveals the use of carbon dioxide, ATP, and NADPH to generate carbohydrates.

14 Stomata are tiny pores in leaves where carbon dioxide enters and oxygen exits.
Student Misconceptions and Concerns 1. Students may understand the overall chemical relationships between photosynthesis and cellular respiration, but many struggle to understand the use of carbon dioxide in the Calvin cycle. Photosynthesis is much more than gas exchange. 2. Students may not connect the growth in plant mass to the fixation of carbon during the Calvin cycle. It can be difficult for many students to appreciate that molecules in air can contribute significantly to the mass of plants. Teaching Tips 1. When introducing the diverse ways that plants impact our lives, consider challenging your students to come up with a list of products made from plants that they encounter regularly. Perhaps you might only list those encountered in a single day of college life. The list can be surprising and help to build up your “catalog of examples.” 2. Within the living world, there are many examples of adaptations to increase surface area. Some examples are the many folds of the inner mitochondrial membrane, the highly branched surfaces of fish gills and human lungs, and the highly branched system of capillaries in the tissues of our bodies. Consider relating this broad principle seen elsewhere to the extensive folding of the thylakoid membranes. 3. In our world, energy is frequently converted to a usable form in one place and used in another. For example, electricity is generated by power plants, transferred to our homes, and used to run computers, create light, and help us prepare foods. Consider relating this common energy transfer to the two-stage process of photosynthesis. 4. You might wish to discuss the evolution of chloroplasts from photosynthetic prokaryotes if you will not address this subject elsewhere in your course. 5. Students who have not read all of chapter 7 may not realize that glucose is not the direct product of photosynthesis. Although glucose is shown as a product of photosynthesis, a three-carbon sugar is directly produced (G3P). A plant can use G3P to make many types of organic molecules, including glucose. (The authors address the production of G3P under the section “The Calvin Cycle” later in this chapter.) 6. Figure 7.3 is an important visual organizer that notes the key structures and functions of the two stages of photosynthesis. This figure reminds students where water and sunlight are used in the thylakoid membranes to generate oxygen, ATP, and NADPH. The second step, in the stroma, reveals the use of carbon dioxide, ATP, and NADPH to generate carbohydrates.

15 Vein Stomata Leaf cross section Figure 7.2-1
CO2 Stomata O2 Leaf cross section Figure 7.2-1 Figure 7.2 Journey into a leaf (Step 1)

16 Inner membrane Outer membrane Chloroplast Vein Granum Stroma Thylakoid
CO2 Stomata O2 LM Leaf cross section Interior cell TEM Figure 7.2-2 Figure 7.2 Journey into a leaf (Step 2)

17 CO2 O2 Vein Stomata Leaf cross section Figure 7.2a
Figure 7.2a Leaf cross section

18 Chloroplast Inner membrane Outer membrane Granum Stroma Thylakoid
TEM Figure 7.2b Figure 7.2b Chloroplast

19 The Overall Equation for Photosynthesis
In the overall equation for photosynthesis, notice that: The reactants of photosynthesis are the waste products of cellular respiration. Student Misconceptions and Concerns 1. Students may understand the overall chemical relationships between photosynthesis and cellular respiration, but many struggle to understand the use of carbon dioxide in the Calvin cycle. Photosynthesis is much more than gas exchange. 2. Students may not connect the growth in plant mass to the fixation of carbon during the Calvin cycle. It can be difficult for many students to appreciate that molecules in air can contribute significantly to the mass of plants. Teaching Tips 1. When introducing the diverse ways that plants impact our lives, consider challenging your students to come up with a list of products made from plants that they encounter regularly. Perhaps you might only list those encountered in a single day of college life. The list can be surprising and help to build up your “catalog of examples.” 2. Within the living world, there are many examples of adaptations to increase surface area. Some examples are the many folds of the inner mitochondrial membrane, the highly branched surfaces of fish gills and human lungs, and the highly branched system of capillaries in the tissues of our bodies. Consider relating this broad principle seen elsewhere to the extensive folding of the thylakoid membranes. 3. In our world, energy is frequently converted to a usable form in one place and used in another. For example, electricity is generated by power plants, transferred to our homes, and used to run computers, create light, and help us prepare foods. Consider relating this common energy transfer to the two-stage process of photosynthesis. 4. You might wish to discuss the evolution of chloroplasts from photosynthetic prokaryotes if you will not address this subject elsewhere in your course. 5. Students who have not read all of chapter 7 may not realize that glucose is not the direct product of photosynthesis. Although glucose is shown as a product of photosynthesis, a three-carbon sugar is directly produced (G3P). A plant can use G3P to make many types of organic molecules, including glucose. (The authors address the production of G3P under the section “The Calvin Cycle” later in this chapter.) 6. Figure 7.3 is an important visual organizer that notes the key structures and functions of the two stages of photosynthesis. This figure reminds students where water and sunlight are used in the thylakoid membranes to generate oxygen, ATP, and NADPH. The second step, in the stroma, reveals the use of carbon dioxide, ATP, and NADPH to generate carbohydrates.

20 Light energy 6 CO2 6 H2O C6H12O6 6 O2 Photo- synthesis Carbon dioxide
Water Glucose Oxygen gas Figure 7.UN1 Figure 7.UN1 Photosynthesis equation

21 In photosynthesis: Sunlight provides the energy
Electrons are boosted “uphill” and added to carbon dioxide Sugar is produced Student Misconceptions and Concerns 1. Students may understand the overall chemical relationships between photosynthesis and cellular respiration, but many struggle to understand the use of carbon dioxide in the Calvin cycle. Photosynthesis is much more than gas exchange. 2. Students may not connect the growth in plant mass to the fixation of carbon during the Calvin cycle. It can be difficult for many students to appreciate that molecules in air can contribute significantly to the mass of plants. Teaching Tips 1. When introducing the diverse ways that plants impact our lives, consider challenging your students to come up with a list of products made from plants that they encounter regularly. Perhaps you might only list those encountered in a single day of college life. The list can be surprising and help to build up your “catalog of examples.” 2. Within the living world, there are many examples of adaptations to increase surface area. Some examples are the many folds of the inner mitochondrial membrane, the highly branched surfaces of fish gills and human lungs, and the highly branched system of capillaries in the tissues of our bodies. Consider relating this broad principle seen elsewhere to the extensive folding of the thylakoid membranes. 3. In our world, energy is frequently converted to a usable form in one place and used in another. For example, electricity is generated by power plants, transferred to our homes, and used to run computers, create light, and help us prepare foods. Consider relating this common energy transfer to the two-stage process of photosynthesis. 4. You might wish to discuss the evolution of chloroplasts from photosynthetic prokaryotes if you will not address this subject elsewhere in your course. 5. Students who have not read all of chapter 7 may not realize that glucose is not the direct product of photosynthesis. Although glucose is shown as a product of photosynthesis, a three-carbon sugar is directly produced (G3P). A plant can use G3P to make many types of organic molecules, including glucose. (The authors address the production of G3P under the section “The Calvin Cycle” later in this chapter.) 6. Figure 7.3 is an important visual organizer that notes the key structures and functions of the two stages of photosynthesis. This figure reminds students where water and sunlight are used in the thylakoid membranes to generate oxygen, ATP, and NADPH. The second step, in the stroma, reveals the use of carbon dioxide, ATP, and NADPH to generate carbohydrates.

22 During photosynthesis, water is split into:
Hydrogen Oxygen Hydrogen is transferred along with electrons and added to carbon dioxide to produce sugar. Oxygen escapes through stomata into the atmosphere. Student Misconceptions and Concerns 1. Students may understand the overall chemical relationships between photosynthesis and cellular respiration, but many struggle to understand the use of carbon dioxide in the Calvin cycle. Photosynthesis is much more than gas exchange. 2. Students may not connect the growth in plant mass to the fixation of carbon during the Calvin cycle. It can be difficult for many students to appreciate that molecules in air can contribute significantly to the mass of plants. Teaching Tips 1. When introducing the diverse ways that plants impact our lives, consider challenging your students to come up with a list of products made from plants that they encounter regularly. Perhaps you might only list those encountered in a single day of college life. The list can be surprising and help to build up your “catalog of examples.” 2. Within the living world, there are many examples of adaptations to increase surface area. Some examples are the many folds of the inner mitochondrial membrane, the highly branched surfaces of fish gills and human lungs, and the highly branched system of capillaries in the tissues of our bodies. Consider relating this broad principle seen elsewhere to the extensive folding of the thylakoid membranes. 3. In our world, energy is frequently converted to a usable form in one place and used in another. For example, electricity is generated by power plants, transferred to our homes, and used to run computers, create light, and help us prepare foods. Consider relating this common energy transfer to the two-stage process of photosynthesis. 4. You might wish to discuss the evolution of chloroplasts from photosynthetic prokaryotes if you will not address this subject elsewhere in your course. 5. Students who have not read all of chapter 7 may not realize that glucose is not the direct product of photosynthesis. Although glucose is shown as a product of photosynthesis, a three-carbon sugar is directly produced (G3P). A plant can use G3P to make many types of organic molecules, including glucose. (The authors address the production of G3P under the section “The Calvin Cycle” later in this chapter.) 6. Figure 7.3 is an important visual organizer that notes the key structures and functions of the two stages of photosynthesis. This figure reminds students where water and sunlight are used in the thylakoid membranes to generate oxygen, ATP, and NADPH. The second step, in the stroma, reveals the use of carbon dioxide, ATP, and NADPH to generate carbohydrates.

23 A Photosynthesis Road Map
Photosynthesis occurs in two stages: The light reactions convert solar energy to chemical energy The Calvin cycle uses the products of the light reactions to make sugar from carbon dioxide Blast Animation: Photosynthesis: Light-Independent Reaction Student Misconceptions and Concerns 1. Students may understand the overall chemical relationships between photosynthesis and cellular respiration, but many struggle to understand the use of carbon dioxide in the Calvin cycle. Photosynthesis is much more than gas exchange. 2. Students may not connect the growth in plant mass to the fixation of carbon during the Calvin cycle. It can be difficult for many students to appreciate that molecules in air can contribute significantly to the mass of plants. Teaching Tips 1. When introducing the diverse ways that plants impact our lives, consider challenging your students to come up with a list of products made from plants that they encounter regularly. Perhaps you might only list those encountered in a single day of college life. The list can be surprising and help to build up your “catalog of examples.” 2. Within the living world, there are many examples of adaptations to increase surface area. Some examples are the many folds of the inner mitochondrial membrane, the highly branched surfaces of fish gills and human lungs, and the highly branched system of capillaries in the tissues of our bodies. Consider relating this broad principle seen elsewhere to the extensive folding of the thylakoid membranes. 3. In our world, energy is frequently converted to a usable form in one place and used in another. For example, electricity is generated by power plants, transferred to our homes, and used to run computers, create light, and help us prepare foods. Consider relating this common energy transfer to the two-stage process of photosynthesis. 4. You might wish to discuss the evolution of chloroplasts from photosynthetic prokaryotes if you will not address this subject elsewhere in your course. 5. Students who have not read all of chapter 7 may not realize that glucose is not the direct product of photosynthesis. Although glucose is shown as a product of photosynthesis, a three-carbon sugar is directly produced (G3P). A plant can use G3P to make many types of organic molecules, including glucose. (The authors address the production of G3P under the section “The Calvin Cycle” later in this chapter.) 6. Figure 7.3 is an important visual organizer that notes the key structures and functions of the two stages of photosynthesis. This figure reminds students where water and sunlight are used in the thylakoid membranes to generate oxygen, ATP, and NADPH. The second step, in the stroma, reveals the use of carbon dioxide, ATP, and NADPH to generate carbohydrates.

24 H2O Light Light reactions O2 ATP NADPH Chloroplast Figure 7.3-1
Figure 7.3 Photosynthesis roadmap (Step 1)

25 H2O CO2 Light Calvin Light cycle reactions O2 NADP+ ADP P ATP NADPH
Chloroplast NADP+ ADP P Calvin cycle Light reactions ATP NADPH O2 Sugar (C6H12O6) Figure 7.3-2 Figure 7.3 Photosynthesis roadmap (Step 2)

26 THE LIGHT REACTIONS: CONVERTING SOLAR ENERGY TO CHEMICAL ENERGY
Chloroplasts: Are chemical factories powered by the sun Convert solar energy into chemical energy © 2010 Pearson Education, Inc. Student Misconceptions and Concerns 1. The authors note that sunlight is a type of radiation. Many students think of radiation as a result of radioactive decay, a serious threat to health. The diverse types of radiation and the varying energy associated with each, might need to be explained. 2. The authors note that electromagnetic energy travels through space in waves that are like those made by a pebble dropped in a pond. This wave imagery is helpful, but can confuse students when energy is also thought of as discrete packets called photons. The dual nature of light as a wave and particle may need to be discussed further, if students are to do more than just accept the definitions. 3. The light reactions are not solely responsible for all of the products in the general chemical equation of photosynthesis. Point out that oxygen is generated in the light reactions but that glucose results from products of the Calvin cycle. Teaching Tips 1. Consider bringing a prism to class and demonstrating the spectrum of visible light. Depending on what you have available, it can be a dramatic reinforcement of this key concept. You might show an image of a rainbow if you are willing to explain how it is produced. 2. Consider bringing a glow stick to class just to help illustrate the authors’ note about the light-generating reactions in glow sticks. Small demonstrations break up lectures and stir attention. 3. Here is a fundamental explanation for a phenomenon that most students have noticed: dark surfaces heat up faster in the sun than do lighter-colored surfaces. This is an opportunity to demonstrate to your students the various depths of scientific explanations and help them to appreciate their own educational progress. In elementary school, they might have learned that the sun heats darker surfaces faster than lighter surfaces. At high school, they may have learned about light energy and that dark surfaces absorb more of this energy than lighter surfaces. Now, in college, they are learning that at the atomic level, darker surfaces absorb the energy of more photons, exciting more electrons, which then fall back to a lower state, releasing more heat. 4. The authors develop a mechanical analogy to the energy levels and movement of electrons in the light reaction. Figure 7.12 equates the height of an electron with its energy state. Thus, electrons captured at high levels carry more energy than electrons in lower positions. Although this figure can be very effective, students might need to be carefully led through the analogy to understand precisely what is represented. 5. The authors note the similarities between oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. If your students have not already read or discussed chemiosmosis in mitochondria, consider assigning the relevant parts of chapter 6 (including Figure 6.11) to show the similarities of these processes.

27 The Nature of Sunlight Sunlight is a type of energy called radiation, or electromagnetic energy. The full range of radiation is called the electromagnetic spectrum. Student Misconceptions and Concerns 1. The authors note that sunlight is a type of radiation. Many students think of radiation as a result of radioactive decay, a serious threat to health. The diverse types of radiation and the varying energy associated with each, might need to be explained. 2. The authors note that electromagnetic energy travels through space in waves that are like those made by a pebble dropped in a pond. This wave imagery is helpful, but can confuse students when energy is also thought of as discrete packets called photons. The dual nature of light as a wave and particle may need to be discussed further, if students are to do more than just accept the definitions. 3. The light reactions are not solely responsible for all of the products in the general chemical equation of photosynthesis. Point out that oxygen is generated in the light reactions but that glucose results from products of the Calvin cycle. Teaching Tips 1. Consider bringing a prism to class and demonstrating the spectrum of visible light. Depending on what you have available, it can be a dramatic reinforcement of this key concept. You might show an image of a rainbow if you are willing to explain how it is produced. 2. Consider bringing a glow stick to class just to help illustrate the authors’ note about the light-generating reactions in glow sticks. Small demonstrations break up lectures and stir attention. 3. Here is a fundamental explanation for a phenomenon that most students have noticed: dark surfaces heat up faster in the sun than do lighter-colored surfaces. This is an opportunity to demonstrate to your students the various depths of scientific explanations and help them to appreciate their own educational progress. In elementary school, they might have learned that the sun heats darker surfaces faster than lighter surfaces. At high school, they may have learned about light energy and that dark surfaces absorb more of this energy than lighter surfaces. Now, in college, they are learning that at the atomic level, darker surfaces absorb the energy of more photons, exciting more electrons, which then fall back to a lower state, releasing more heat. 4. The authors develop a mechanical analogy to the energy levels and movement of electrons in the light reaction. Figure 7.12 equates the height of an electron with its energy state. Thus, electrons captured at high levels carry more energy than electrons in lower positions. Although this figure can be very effective, students might need to be carefully led through the analogy to understand precisely what is represented. 5. The authors note the similarities between oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. If your students have not already read or discussed chemiosmosis in mitochondria, consider assigning the relevant parts of chapter 6 (including Figure 6.11) to show the similarities of these processes.

28 Micro- waves Radio waves Infrared
Increasing wavelength 10–5 nm 10–3 nm 1 nm 103 nm 106 nm 1 m 103 m Micro- waves Radio waves Gamma rays X-rays UV Infrared Visible light 500 600 700 750 Wavelength (nm) Wavelength = 580 nm Figure 7.4 Figure 7.4 The electromagnetic spectrum

29 The Process of Science: What Colors of Light Drive Photosynthesis?
Observation: In 1883, German biologist Theodor Engelmann saw that certain bacteria tend to cluster in areas with higher oxygen concentrations. Question: Could this information determine which wavelengths of light work best for photosynthesis? Hypothesis: Oxygen-seeking bacteria will congregate near regions of algae performing the most photosynthesis. © 2010 Pearson Education, Inc. Student Misconceptions and Concerns 1. The authors note that sunlight is a type of radiation. Many students think of radiation as a result of radioactive decay, a serious threat to health. The diverse types of radiation and the varying energy associated with each, might need to be explained. 2. The authors note that electromagnetic energy travels through space in waves that are like those made by a pebble dropped in a pond. This wave imagery is helpful, but can confuse students when energy is also thought of as discrete packets called photons. The dual nature of light as a wave and particle may need to be discussed further, if students are to do more than just accept the definitions. 3. The light reactions are not solely responsible for all of the products in the general chemical equation of photosynthesis. Point out that oxygen is generated in the light reactions but that glucose results from products of the Calvin cycle. Teaching Tips 1. Consider bringing a prism to class and demonstrating the spectrum of visible light. Depending on what you have available, it can be a dramatic reinforcement of this key concept. You might show an image of a rainbow if you are willing to explain how it is produced. 2. Consider bringing a glow stick to class just to help illustrate the authors’ note about the light-generating reactions in glow sticks. Small demonstrations break up lectures and stir attention. 3. Here is a fundamental explanation for a phenomenon that most students have noticed: dark surfaces heat up faster in the sun than do lighter-colored surfaces. This is an opportunity to demonstrate to your students the various depths of scientific explanations and help them to appreciate their own educational progress. In elementary school, they might have learned that the sun heats darker surfaces faster than lighter surfaces. At high school, they may have learned about light energy and that dark surfaces absorb more of this energy than lighter surfaces. Now, in college, they are learning that at the atomic level, darker surfaces absorb the energy of more photons, exciting more electrons, which then fall back to a lower state, releasing more heat. 4. The authors develop a mechanical analogy to the energy levels and movement of electrons in the light reaction. Figure 7.12 equates the height of an electron with its energy state. Thus, electrons captured at high levels carry more energy than electrons in lower positions. Although this figure can be very effective, students might need to be carefully led through the analogy to understand precisely what is represented. 5. The authors note the similarities between oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. If your students have not already read or discussed chemiosmosis in mitochondria, consider assigning the relevant parts of chapter 6 (including Figure 6.11) to show the similarities of these processes.

30 Experiment: Engelmann:
Laid a string of freshwater algal cells in a drop of water on a microscope slide Added oxygen-sensitive bacteria to the drop Used a prism to create a spectrum of light shining on the slide Student Misconceptions and Concerns 1. The authors note that sunlight is a type of radiation. Many students think of radiation as a result of radioactive decay, a serious threat to health. The diverse types of radiation and the varying energy associated with each, might need to be explained. 2. The authors note that electromagnetic energy travels through space in waves that are like those made by a pebble dropped in a pond. This wave imagery is helpful, but can confuse students when energy is also thought of as discrete packets called photons. The dual nature of light as a wave and particle may need to be discussed further, if students are to do more than just accept the definitions. 3. The light reactions are not solely responsible for all of the products in the general chemical equation of photosynthesis. Point out that oxygen is generated in the light reactions but that glucose results from products of the Calvin cycle. Teaching Tips 1. Consider bringing a prism to class and demonstrating the spectrum of visible light. Depending on what you have available, it can be a dramatic reinforcement of this key concept. You might show an image of a rainbow if you are willing to explain how it is produced. 2. Consider bringing a glow stick to class just to help illustrate the authors’ note about the light-generating reactions in glow sticks. Small demonstrations break up lectures and stir attention. 3. Here is a fundamental explanation for a phenomenon that most students have noticed: dark surfaces heat up faster in the sun than do lighter-colored surfaces. This is an opportunity to demonstrate to your students the various depths of scientific explanations and help them to appreciate their own educational progress. In elementary school, they might have learned that the sun heats darker surfaces faster than lighter surfaces. At high school, they may have learned about light energy and that dark surfaces absorb more of this energy than lighter surfaces. Now, in college, they are learning that at the atomic level, darker surfaces absorb the energy of more photons, exciting more electrons, which then fall back to a lower state, releasing more heat. 4. The authors develop a mechanical analogy to the energy levels and movement of electrons in the light reaction. Figure 7.12 equates the height of an electron with its energy state. Thus, electrons captured at high levels carry more energy than electrons in lower positions. Although this figure can be very effective, students might need to be carefully led through the analogy to understand precisely what is represented. 5. The authors note the similarities between oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. If your students have not already read or discussed chemiosmosis in mitochondria, consider assigning the relevant parts of chapter 6 (including Figure 6.11) to show the similarities of these processes.

31 Animation: Light and Pigments
Results: Bacteria: Mostly congregated around algae exposed to red-orange and blue-violet light Rarely moved to areas of green light Conclusion: Chloroplasts absorb light mainly in the blue-violet and red-orange part of the spectrum. Animation: Light and Pigments Student Misconceptions and Concerns 1. The authors note that sunlight is a type of radiation. Many students think of radiation as a result of radioactive decay, a serious threat to health. The diverse types of radiation and the varying energy associated with each, might need to be explained. 2. The authors note that electromagnetic energy travels through space in waves that are like those made by a pebble dropped in a pond. This wave imagery is helpful, but can confuse students when energy is also thought of as discrete packets called photons. The dual nature of light as a wave and particle may need to be discussed further, if students are to do more than just accept the definitions. 3. The light reactions are not solely responsible for all of the products in the general chemical equation of photosynthesis. Point out that oxygen is generated in the light reactions but that glucose results from products of the Calvin cycle. Teaching Tips 1. Consider bringing a prism to class and demonstrating the spectrum of visible light. Depending on what you have available, it can be a dramatic reinforcement of this key concept. You might show an image of a rainbow if you are willing to explain how it is produced. 2. Consider bringing a glow stick to class just to help illustrate the authors’ note about the light-generating reactions in glow sticks. Small demonstrations break up lectures and stir attention. 3. Here is a fundamental explanation for a phenomenon that most students have noticed: dark surfaces heat up faster in the sun than do lighter-colored surfaces. This is an opportunity to demonstrate to your students the various depths of scientific explanations and help them to appreciate their own educational progress. In elementary school, they might have learned that the sun heats darker surfaces faster than lighter surfaces. At high school, they may have learned about light energy and that dark surfaces absorb more of this energy than lighter surfaces. Now, in college, they are learning that at the atomic level, darker surfaces absorb the energy of more photons, exciting more electrons, which then fall back to a lower state, releasing more heat. 4. The authors develop a mechanical analogy to the energy levels and movement of electrons in the light reaction. Figure 7.12 equates the height of an electron with its energy state. Thus, electrons captured at high levels carry more energy than electrons in lower positions. Although this figure can be very effective, students might need to be carefully led through the analogy to understand precisely what is represented. 5. The authors note the similarities between oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. If your students have not already read or discussed chemiosmosis in mitochondria, consider assigning the relevant parts of chapter 6 (including Figure 6.11) to show the similarities of these processes.

32 Wavelength of light (nm)
Prism Microscope slide Bacterium Number of bacteria Algal cells 400 500 600 700 Wavelength of light (nm) Figure 7.5 Figure 7.5 Investigating how light wavelength affects photosynthesis

33 Light Reflected light Chloroplast Absorbed light Transmitted light
Figure 7.6 Figure 7.6 Why are leaves green?

34 Light Reflected light Chloroplast Absorbed light Transmitted light
Figure 7.6a Figure 7.6a Why are leaves green?

35 Figure 7.6b Figure 7.6b Leaves in sunlight

36 Chloroplast Pigments Chloroplasts contain several pigments:
Chlorophyll a: Absorbs mostly blue-violet and red light Participates directly in the light reactions Chlorophyll b: Absorbs mostly blue and orange light Participates indirectly in the light reactions Student Misconceptions and Concerns 1. The authors note that sunlight is a type of radiation. Many students think of radiation as a result of radioactive decay, a serious threat to health. The diverse types of radiation and the varying energy associated with each, might need to be explained. 2. The authors note that electromagnetic energy travels through space in waves that are like those made by a pebble dropped in a pond. This wave imagery is helpful, but can confuse students when energy is also thought of as discrete packets called photons. The dual nature of light as a wave and particle may need to be discussed further, if students are to do more than just accept the definitions. 3. The light reactions are not solely responsible for all of the products in the general chemical equation of photosynthesis. Point out that oxygen is generated in the light reactions but that glucose results from products of the Calvin cycle. Teaching Tips 1. Consider bringing a prism to class and demonstrating the spectrum of visible light. Depending on what you have available, it can be a dramatic reinforcement of this key concept. You might show an image of a rainbow if you are willing to explain how it is produced. 2. Consider bringing a glow stick to class just to help illustrate the authors’ note about the light-generating reactions in glow sticks. Small demonstrations break up lectures and stir attention. 3. Here is a fundamental explanation for a phenomenon that most students have noticed: dark surfaces heat up faster in the sun than do lighter-colored surfaces. This is an opportunity to demonstrate to your students the various depths of scientific explanations and help them to appreciate their own educational progress. In elementary school, they might have learned that the sun heats darker surfaces faster than lighter surfaces. At high school, they may have learned about light energy and that dark surfaces absorb more of this energy than lighter surfaces. Now, in college, they are learning that at the atomic level, darker surfaces absorb the energy of more photons, exciting more electrons, which then fall back to a lower state, releasing more heat. 4. The authors develop a mechanical analogy to the energy levels and movement of electrons in the light reaction. Figure 7.12 equates the height of an electron with its energy state. Thus, electrons captured at high levels carry more energy than electrons in lower positions. Although this figure can be very effective, students might need to be carefully led through the analogy to understand precisely what is represented. 5. The authors note the similarities between oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. If your students have not already read or discussed chemiosmosis in mitochondria, consider assigning the relevant parts of chapter 6 (including Figure 6.11) to show the similarities of these processes.

37 Absorb mainly blue-green light
Carotenoids: Absorb mainly blue-green light Participate indirectly in the light reactions Absorb and dissipate excessive light energy that might damage chlorophyll The spectacular colors of fall foliage are due partly to the yellow-orange light reflected from carotenoids. Student Misconceptions and Concerns 1. The authors note that sunlight is a type of radiation. Many students think of radiation as a result of radioactive decay, a serious threat to health. The diverse types of radiation and the varying energy associated with each, might need to be explained. 2. The authors note that electromagnetic energy travels through space in waves that are like those made by a pebble dropped in a pond. This wave imagery is helpful, but can confuse students when energy is also thought of as discrete packets called photons. The dual nature of light as a wave and particle may need to be discussed further, if students are to do more than just accept the definitions. 3. The light reactions are not solely responsible for all of the products in the general chemical equation of photosynthesis. Point out that oxygen is generated in the light reactions but that glucose results from products of the Calvin cycle. Teaching Tips 1. Consider bringing a prism to class and demonstrating the spectrum of visible light. Depending on what you have available, it can be a dramatic reinforcement of this key concept. You might show an image of a rainbow if you are willing to explain how it is produced. 2. Consider bringing a glow stick to class just to help illustrate the authors’ note about the light-generating reactions in glow sticks. Small demonstrations break up lectures and stir attention. 3. Here is a fundamental explanation for a phenomenon that most students have noticed: dark surfaces heat up faster in the sun than do lighter-colored surfaces. This is an opportunity to demonstrate to your students the various depths of scientific explanations and help them to appreciate their own educational progress. In elementary school, they might have learned that the sun heats darker surfaces faster than lighter surfaces. At high school, they may have learned about light energy and that dark surfaces absorb more of this energy than lighter surfaces. Now, in college, they are learning that at the atomic level, darker surfaces absorb the energy of more photons, exciting more electrons, which then fall back to a lower state, releasing more heat. 4. The authors develop a mechanical analogy to the energy levels and movement of electrons in the light reaction. Figure 7.12 equates the height of an electron with its energy state. Thus, electrons captured at high levels carry more energy than electrons in lower positions. Although this figure can be very effective, students might need to be carefully led through the analogy to understand precisely what is represented. 5. The authors note the similarities between oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. If your students have not already read or discussed chemiosmosis in mitochondria, consider assigning the relevant parts of chapter 6 (including Figure 6.11) to show the similarities of these processes.

38 Figure 7.7 Figure 7.7 Photosynthetic pigments

39 How Photosystems Harvest Light Energy
Light behaves as photons, discrete packets of energy. Student Misconceptions and Concerns 1. The authors note that sunlight is a type of radiation. Many students think of radiation as a result of radioactive decay, a serious threat to health. The diverse types of radiation and the varying energy associated with each, might need to be explained. 2. The authors note that electromagnetic energy travels through space in waves that are like those made by a pebble dropped in a pond. This wave imagery is helpful, but can confuse students when energy is also thought of as discrete packets called photons. The dual nature of light as a wave and particle may need to be discussed further, if students are to do more than just accept the definitions. 3. The light reactions are not solely responsible for all of the products in the general chemical equation of photosynthesis. Point out that oxygen is generated in the light reactions but that glucose results from products of the Calvin cycle. Teaching Tips 1. Consider bringing a prism to class and demonstrating the spectrum of visible light. Depending on what you have available, it can be a dramatic reinforcement of this key concept. You might show an image of a rainbow if you are willing to explain how it is produced. 2. Consider bringing a glow stick to class just to help illustrate the authors’ note about the light-generating reactions in glow sticks. Small demonstrations break up lectures and stir attention. 3. Here is a fundamental explanation for a phenomenon that most students have noticed: dark surfaces heat up faster in the sun than do lighter-colored surfaces. This is an opportunity to demonstrate to your students the various depths of scientific explanations and help them to appreciate their own educational progress. In elementary school, they might have learned that the sun heats darker surfaces faster than lighter surfaces. At high school, they may have learned about light energy and that dark surfaces absorb more of this energy than lighter surfaces. Now, in college, they are learning that at the atomic level, darker surfaces absorb the energy of more photons, exciting more electrons, which then fall back to a lower state, releasing more heat. 4. The authors develop a mechanical analogy to the energy levels and movement of electrons in the light reaction. Figure 7.12 equates the height of an electron with its energy state. Thus, electrons captured at high levels carry more energy than electrons in lower positions. Although this figure can be very effective, students might need to be carefully led through the analogy to understand precisely what is represented. 5. The authors note the similarities between oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. If your students have not already read or discussed chemiosmosis in mitochondria, consider assigning the relevant parts of chapter 6 (including Figure 6.11) to show the similarities of these processes.

40 Chlorophyll molecules absorb photons.
Electrons in the pigment gain energy. As the electrons fall back to their ground state, energy is released as heat or light. Student Misconceptions and Concerns 1. The authors note that sunlight is a type of radiation. Many students think of radiation as a result of radioactive decay, a serious threat to health. The diverse types of radiation and the varying energy associated with each, might need to be explained. 2. The authors note that electromagnetic energy travels through space in waves that are like those made by a pebble dropped in a pond. This wave imagery is helpful, but can confuse students when energy is also thought of as discrete packets called photons. The dual nature of light as a wave and particle may need to be discussed further, if students are to do more than just accept the definitions. 3. The light reactions are not solely responsible for all of the products in the general chemical equation of photosynthesis. Point out that oxygen is generated in the light reactions but that glucose results from products of the Calvin cycle. Teaching Tips 1. Consider bringing a prism to class and demonstrating the spectrum of visible light. Depending on what you have available, it can be a dramatic reinforcement of this key concept. You might show an image of a rainbow if you are willing to explain how it is produced. 2. Consider bringing a glow stick to class just to help illustrate the authors’ note about the light-generating reactions in glow sticks. Small demonstrations break up lectures and stir attention. 3. Here is a fundamental explanation for a phenomenon that most students have noticed: dark surfaces heat up faster in the sun than do lighter-colored surfaces. This is an opportunity to demonstrate to your students the various depths of scientific explanations and help them to appreciate their own educational progress. In elementary school, they might have learned that the sun heats darker surfaces faster than lighter surfaces. At high school, they may have learned about light energy and that dark surfaces absorb more of this energy than lighter surfaces. Now, in college, they are learning that at the atomic level, darker surfaces absorb the energy of more photons, exciting more electrons, which then fall back to a lower state, releasing more heat. 4. The authors develop a mechanical analogy to the energy levels and movement of electrons in the light reaction. Figure 7.12 equates the height of an electron with its energy state. Thus, electrons captured at high levels carry more energy than electrons in lower positions. Although this figure can be very effective, students might need to be carefully led through the analogy to understand precisely what is represented. 5. The authors note the similarities between oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. If your students have not already read or discussed chemiosmosis in mitochondria, consider assigning the relevant parts of chapter 6 (including Figure 6.11) to show the similarities of these processes.

41 (a) Absorption Excited state of a photon Light Heat
Light (fluorescence) Photon Ground state Chlorophyll molecule (b) Fluorescence of a glow stick Figure 7.8 Figure 7.8 Excited electrons in pigments

42 e– Excited state Light Heat Light (fluorescence) Photon Ground state
Chlorophyll molecule (a) Absorption of a photon Figure 7.8a Figure 7.8a Absorption of a photon

43 (b) Fluorescence of a glow stick
Figure 7.8b Figure 7.8b Fluorescence of a glow stick

44 A photosystem is a group of chlorophyll and other molecules that function as a light-gathering antenna. Student Misconceptions and Concerns 1. The authors note that sunlight is a type of radiation. Many students think of radiation as a result of radioactive decay, a serious threat to health. The diverse types of radiation and the varying energy associated with each, might need to be explained. 2. The authors note that electromagnetic energy travels through space in waves that are like those made by a pebble dropped in a pond. This wave imagery is helpful, but can confuse students when energy is also thought of as discrete packets called photons. The dual nature of light as a wave and particle may need to be discussed further, if students are to do more than just accept the definitions. 3. The light reactions are not solely responsible for all of the products in the general chemical equation of photosynthesis. Point out that oxygen is generated in the light reactions but that glucose results from products of the Calvin cycle. Teaching Tips 1. Consider bringing a prism to class and demonstrating the spectrum of visible light. Depending on what you have available, it can be a dramatic reinforcement of this key concept. You might show an image of a rainbow if you are willing to explain how it is produced. 2. Consider bringing a glow stick to class just to help illustrate the authors’ note about the light-generating reactions in glow sticks. Small demonstrations break up lectures and stir attention. 3. Here is a fundamental explanation for a phenomenon that most students have noticed: dark surfaces heat up faster in the sun than do lighter-colored surfaces. This is an opportunity to demonstrate to your students the various depths of scientific explanations and help them to appreciate their own educational progress. In elementary school, they might have learned that the sun heats darker surfaces faster than lighter surfaces. At high school, they may have learned about light energy and that dark surfaces absorb more of this energy than lighter surfaces. Now, in college, they are learning that at the atomic level, darker surfaces absorb the energy of more photons, exciting more electrons, which then fall back to a lower state, releasing more heat. 4. The authors develop a mechanical analogy to the energy levels and movement of electrons in the light reaction. Figure 7.12 equates the height of an electron with its energy state. Thus, electrons captured at high levels carry more energy than electrons in lower positions. Although this figure can be very effective, students might need to be carefully led through the analogy to understand precisely what is represented. 5. The authors note the similarities between oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. If your students have not already read or discussed chemiosmosis in mitochondria, consider assigning the relevant parts of chapter 6 (including Figure 6.11) to show the similarities of these processes.

45 Chloroplast Figure 7.9-1 Figure 7.9 A photosystem (Step 1)

46 Chloroplast Thylakoid membrane Figure 7.9-2 Pigment molecules
Figure 7.9 A photosystem (Step 2)

47 Chloroplast Thylakoid membrane Photosystem Transfer of energy
Pigment molecules Photon Primary electron acceptor Reaction center Electron transfer Reaction- center chlorophyll a Antenna pigment molecules Transfer of energy Thylakoid membrane Photosystem Figure 7.9-3 Figure 7.9 A photosystem (Step 3)

48 How the Light Reactions Generate ATP and NADPH
Two types of photosystems cooperate in the light reactions: The water-splitting photosystem The NADPH-producing photosystem Student Misconceptions and Concerns 1. The authors note that sunlight is a type of radiation. Many students think of radiation as a result of radioactive decay, a serious threat to health. The diverse types of radiation and the varying energy associated with each, might need to be explained. 2. The authors note that electromagnetic energy travels through space in waves that are like those made by a pebble dropped in a pond. This wave imagery is helpful, but can confuse students when energy is also thought of as discrete packets called photons. The dual nature of light as a wave and particle may need to be discussed further, if students are to do more than just accept the definitions. 3. The light reactions are not solely responsible for all of the products in the general chemical equation of photosynthesis. Point out that oxygen is generated in the light reactions but that glucose results from products of the Calvin cycle. Teaching Tips 1. Consider bringing a prism to class and demonstrating the spectrum of visible light. Depending on what you have available, it can be a dramatic reinforcement of this key concept. You might show an image of a rainbow if you are willing to explain how it is produced. 2. Consider bringing a glow stick to class just to help illustrate the authors’ note about the light-generating reactions in glow sticks. Small demonstrations break up lectures and stir attention. 3. Here is a fundamental explanation for a phenomenon that most students have noticed: dark surfaces heat up faster in the sun than do lighter-colored surfaces. This is an opportunity to demonstrate to your students the various depths of scientific explanations and help them to appreciate their own educational progress. In elementary school, they might have learned that the sun heats darker surfaces faster than lighter surfaces. At high school, they may have learned about light energy and that dark surfaces absorb more of this energy than lighter surfaces. Now, in college, they are learning that at the atomic level, darker surfaces absorb the energy of more photons, exciting more electrons, which then fall back to a lower state, releasing more heat. 4. The authors develop a mechanical analogy to the energy levels and movement of electrons in the light reaction. Figure 7.12 equates the height of an electron with its energy state. Thus, electrons captured at high levels carry more energy than electrons in lower positions. Although this figure can be very effective, students might need to be carefully led through the analogy to understand precisely what is represented. 5. The authors note the similarities between oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. If your students have not already read or discussed chemiosmosis in mitochondria, consider assigning the relevant parts of chapter 6 (including Figure 6.11) to show the similarities of these processes.

49 Figure 7.10-1 Primary electron acceptor Light Reaction- center
Light Reaction- center chlorophyll H2O 2e Water-splitting photosystem 2 H + + O2 Figure Figure 7.10 Light reactions (Step 1)

50 Electron transport chain
Energy to make ATP Primary electron acceptor 2e Electron transport chain Light Reaction- center chlorophyll H2O 2e Water-splitting photosystem 2 H + + O2 Figure Figure 7.10 Light reactions (Step 2)

51 Electron transport chain
Primary electron acceptor NADP 2e Energy to make ATP Primary electron acceptor 2e NADPH 2e Light Electron transport chain Light Reaction- center chlorophyll Reaction- center chlorophyll NADPH-producing photosystem H2O 2e Water-splitting photosystem 2 H + + O2 Figure Figure 7.10 Light reactions (Step 3)

52 The light reactions are located in the thylakoid membrane.
Student Misconceptions and Concerns 1. The authors note that sunlight is a type of radiation. Many students think of radiation as a result of radioactive decay, a serious threat to health. The diverse types of radiation and the varying energy associated with each, might need to be explained. 2. The authors note that electromagnetic energy travels through space in waves that are like those made by a pebble dropped in a pond. This wave imagery is helpful, but can confuse students when energy is also thought of as discrete packets called photons. The dual nature of light as a wave and particle may need to be discussed further, if students are to do more than just accept the definitions. 3. The light reactions are not solely responsible for all of the products in the general chemical equation of photosynthesis. Point out that oxygen is generated in the light reactions but that glucose results from products of the Calvin cycle. Teaching Tips 1. Consider bringing a prism to class and demonstrating the spectrum of visible light. Depending on what you have available, it can be a dramatic reinforcement of this key concept. You might show an image of a rainbow if you are willing to explain how it is produced. 2. Consider bringing a glow stick to class just to help illustrate the authors’ note about the light-generating reactions in glow sticks. Small demonstrations break up lectures and stir attention. 3. Here is a fundamental explanation for a phenomenon that most students have noticed: dark surfaces heat up faster in the sun than do lighter-colored surfaces. This is an opportunity to demonstrate to your students the various depths of scientific explanations and help them to appreciate their own educational progress. In elementary school, they might have learned that the sun heats darker surfaces faster than lighter surfaces. At high school, they may have learned about light energy and that dark surfaces absorb more of this energy than lighter surfaces. Now, in college, they are learning that at the atomic level, darker surfaces absorb the energy of more photons, exciting more electrons, which then fall back to a lower state, releasing more heat. 4. The authors develop a mechanical analogy to the energy levels and movement of electrons in the light reaction. Figure 7.12 equates the height of an electron with its energy state. Thus, electrons captured at high levels carry more energy than electrons in lower positions. Although this figure can be very effective, students might need to be carefully led through the analogy to understand precisely what is represented. 5. The authors note the similarities between oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. If your students have not already read or discussed chemiosmosis in mitochondria, consider assigning the relevant parts of chapter 6 (including Figure 6.11) to show the similarities of these processes.

53 An electron transport chain:
Connects the two photosystems Releases energy that the chloroplast uses to make ATP Student Misconceptions and Concerns 1. The authors note that sunlight is a type of radiation. Many students think of radiation as a result of radioactive decay, a serious threat to health. The diverse types of radiation and the varying energy associated with each, might need to be explained. 2. The authors note that electromagnetic energy travels through space in waves that are like those made by a pebble dropped in a pond. This wave imagery is helpful, but can confuse students when energy is also thought of as discrete packets called photons. The dual nature of light as a wave and particle may need to be discussed further, if students are to do more than just accept the definitions. 3. The light reactions are not solely responsible for all of the products in the general chemical equation of photosynthesis. Point out that oxygen is generated in the light reactions but that glucose results from products of the Calvin cycle. Teaching Tips 1. Consider bringing a prism to class and demonstrating the spectrum of visible light. Depending on what you have available, it can be a dramatic reinforcement of this key concept. You might show an image of a rainbow if you are willing to explain how it is produced. 2. Consider bringing a glow stick to class just to help illustrate the authors’ note about the light-generating reactions in glow sticks. Small demonstrations break up lectures and stir attention. 3. Here is a fundamental explanation for a phenomenon that most students have noticed: dark surfaces heat up faster in the sun than do lighter-colored surfaces. This is an opportunity to demonstrate to your students the various depths of scientific explanations and help them to appreciate their own educational progress. In elementary school, they might have learned that the sun heats darker surfaces faster than lighter surfaces. At high school, they may have learned about light energy and that dark surfaces absorb more of this energy than lighter surfaces. Now, in college, they are learning that at the atomic level, darker surfaces absorb the energy of more photons, exciting more electrons, which then fall back to a lower state, releasing more heat. 4. The authors develop a mechanical analogy to the energy levels and movement of electrons in the light reaction. Figure 7.12 equates the height of an electron with its energy state. Thus, electrons captured at high levels carry more energy than electrons in lower positions. Although this figure can be very effective, students might need to be carefully led through the analogy to understand precisely what is represented. 5. The authors note the similarities between oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. If your students have not already read or discussed chemiosmosis in mitochondria, consider assigning the relevant parts of chapter 6 (including Figure 6.11) to show the similarities of these processes.

54 Figure 7.11 – To Calvin cycle Light Light Stroma ATP Thylakoid
NADPH ATP ADP P NADP H Stroma Electron transport chain Thylakoid membrane Photosystem Photosystem ATP synthase Inside thylakoid Electron flow 2e H H H2O H H H+ O2 Figure 7.11 Figure 7.11 How the thylakoid membrane converts light energy to the chemical energy of NADPH and ATP

55 – To Calvin cycle Light Light Stroma ATP Thylakoid synthase membrane
NADPH ATP ADP P NADP H Stroma Electron transport chain Thylakoid membrane Photosystem Photosystem ATP synthase Inside thylakoid Electron flow 2e H H H2O H H H O2 Figure 7.11a Figure 7.11a How the thylakoid membrane converts light energy to the chemical energy of NADPH and ATP

56 – – – – – – – ATP NADPH Water-splitting photosystem NADPH-producing
Photon e Photon Water-splitting photosystem NADPH-producing photosystem Figure 7.12 Figure 7.12 A hard-hat analogy for the light reactions

57 THE CALVIN CYCLE: MAKING SUGAR FROM CARBON DIOXIDE
Functions like a sugar factory within the stroma of a chloroplast Regenerates the starting material with each turn © 2010 Pearson Education, Inc. Student Misconceptions and Concerns 1. Glucose is not the direct product of the Calvin cycle, as might be expected from the general equation for photosynthesis. Instead, G3P, as noted in the text, is the main product. Clarify for students the diverse uses of G3P in the production of many important plant molecules and the advantages of producing a molecule with this flexibility. Teaching Tips 1. If you can find examples of potted C3, C4, and CAM plants, consider bringing them to class. Referring to living plants in class helps students understand these abstract concepts. Nice photographs can serve as a substitute. 2. Relate the properties of C3 and C4 plants to the regions of the country where each are grown. Students might generally understand that crops have specific requirements, but may not specifically relate these physiological differences to their geographic sites of production.

58 Three-carbon molecule
CO2 (from air) P RuBP sugar Three-carbon molecule P P Calvin cycle Figure Figure 7.13 The Calvin cycle (Step 1)

59 Three-carbon molecule
CO2 (from air) P RuBP sugar Three-carbon molecule ATP P P ADP P Calvin cycle NADPH NADP G3P sugar P Figure Figure 7.13 The Calvin cycle (Step 2)

60 Three-carbon molecule
CO2 (from air) P RuBP sugar Three-carbon molecule ATP P P ADP P Calvin cycle NADPH NADP G3P sugar G3P sugar P P G3P sugar Glucose (and other organic compounds) P Figure Figure 7.13 The Calvin cycle (Step 3)

61 Three-carbon molecule
CO2 (from air) P RuBP sugar Three-carbon molecule ATP P P ADP P ADP P Calvin cycle NADPH ATP NADP G3P sugar G3P sugar P P G3P sugar Glucose (and other organic compounds) P Figure Figure 7.13 The Calvin cycle (Step 4)

62 Evolution Connection: Solar-Driven Evolution
C3 plants: Use CO2 directly from the air Are very common and widely distributed © 2010 Pearson Education, Inc. Student Misconceptions and Concerns 1. Glucose is not the direct product of the Calvin cycle, as might be expected from the general equation for photosynthesis. Instead, G3P, as noted in the text, is the main product. Clarify for students the diverse uses of G3P in the production of many important plant molecules and the advantages of producing a molecule with this flexibility. Teaching Tips 1. If you can find examples of potted C3, C4, and CAM plants, consider bringing them to class. Referring to living plants in class helps students understand these abstract concepts. Nice photographs can serve as a substitute. 2. Relate the properties of C3 and C4 plants to the regions of the country where each are grown. Students might generally understand that crops have specific requirements, but may not specifically relate these physiological differences to their geographic sites of production.

63 C4 plants: Close their stomata to save water during hot and dry weather Can still carry out photosynthesis CAM plants: Are adapted to very dry climates Open their stomata only at night to conserve water Student Misconceptions and Concerns 1. Glucose is not the direct product of the Calvin cycle, as might be expected from the general equation for photosynthesis. Instead, G3P, as noted in the text, is the main product. Clarify for students the diverse uses of G3P in the production of many important plant molecules and the advantages of producing a molecule with this flexibility. Teaching Tips 1. If you can find examples of potted C3, C4, and CAM plants, consider bringing them to class. Referring to living plants in class helps students understand these abstract concepts. Nice photographs can serve as a substitute. 2. Relate the properties of C3 and C4 plants to the regions of the country where each are grown. Students might generally understand that crops have specific requirements, but may not specifically relate these physiological differences to their geographic sites of production.

64 C4 Pathway (example: sugarcane) CAM Pathway (example: pineapple)
ALTERNATIVE PHOTOSYNTHETIC PATHWAYS C4 Pathway (example: sugarcane) CAM Pathway (example: pineapple) Cell type 1 CO2 CO2 Night Four-carbon compound Four-carbon compound CO2 CO2 Cell type 2 Calvin cycle Calvin cycle Sugar Sugar Day C4 plant CAM plant Figure 7.14 Figure 7.14 C4 and CAM photosynthesis

65 C4 Pathway (example: sugarcane)
Cell type 1 CO2 Four-carbon compound CO2 Cell type 2 Calvin cycle Sugar C4 plant Figure 7.14a Figure 7.14a C4 pathway

66 CAM Pathway (example: pineapple)
CO2 Night Four-carbon compound CO2 Calvin cycle Sugar Day CAM plant Figure 7.14b Figure 7.14b CAM pathway

67 Light energy 6 CO2 6 H2O C6H12O6 6 O2 Photo- synthesis Carbon dioxide
Water Glucose Oxygen gas Figure 7.UN1 Figure 7.UN1 Photosynthesis equation

68 Calvin cycle Light reactions
CO2 H2O Light NADP ADP P Calvin cycle Light reactions ATP NADPH Sugar O2 (C6H12O6) Figure 7.UN2 Figure 7.UN2 Orientation diagram: light reactions

69 Calvin cycle Light reactions
CO2 Light H2O NADP ADP P Calvin cycle Light reactions ATP NADPH Sugar O2 (C6H12O6) Figure 7.UN3 Figure 7.UN3 Orientation diagram: Calvin cycle

70 Light energy Photosynthesis Carbon dioxide Water Glucose Oxygen gas
C6H12O6 6 O2 Photosynthesis Carbon dioxide Water Glucose Oxygen gas Figure 7.UN4 Figure 7.UN4 Summary: photosynthesis equation

71 H2O Chloroplast CO2 Light Stack of thylakoids NADP+ Stroma ADP Calvin
cycle Light reactions ATP NADPH O2 Sugar (C6H12O6) Figure 7.UN5 Figure 7.UN5 Summary: light reactions and Calvin cycle

72 – – NADP+ ADP ATP NADPH Photon Photon H2O NADPH-producing photosystem
acceptor e 2e acceptor 2e NADPH Photon Electron transport chain Photon Chlorophyll H2O Chlorophyll NADPH-producing photosystem 2e Water-splitting photosystem 2 H 2 1 + O2 + Figure 7.UN6 Figure 7.UN6 Summary: light reactions

73 CO2 ATP ADP P Calvin cycle NADPH NADP G3P Glucose and other compounds
Figure 7.UN7 Figure 7.UN7 Summary: Calvin cycle


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