1. Primary Research Paper: Cell & Molecular Biology
What is Zwilch?

2. Questions for Research Article
What is the significance of the research conducted?
What were the questions asked, hypotheses proposed, or goal of the research?
Describe the trends or other descriptive data they presented?
Briefly describe the design of the experiment (e.g. variables, controls, etc.).
What types of statistical analysis was performed on the data?
See handout and website for updates on questions

3. Sections of an Article Title Abstract Intro
Fig … fig legends… results… methods
Discussion
References
Redundant?
See electronic handout on website for more details

4. Tells what was accomplished in the paper Rephrase in your own words
Title
Tells what was accomplished in the paper
Rephrase in your own words
Socrative

5. Abstract System Molecules pertinent to the study Terms Techniques
Hypothesis or Goal
Socrative
What is the system in this paper?

6. Hypothesis
State the hypothesis as an “if this… then” statement
Socrative

7. Hypothesis
Goal: to identify other components (than ZW10/ROD) of the complex necessary for accurate chromosome segregation
-or-
If Zwilch is an important component of the chromosome segregation complex

8. Then… Immunoaffinity chromatography confirm Zwilch part of complex
Mass spectroscopy confirm Zwilch part of complex
Localize to kinetochore & kinetochore microtubules same as ZW10/ROD
Mutation same mitotic phenotypes as zw10/rod
Evolutionarily conserved (drosophila & human same coppt w/ ZW10/ROD and localized to kinetochores at prometaphase)

9. New questions:
weak interaction w/ kinesin-like kinetochore component CENP-meta

10. Introduction Significance Socrative
Is the significance anywhere else in the paper?
State one reason the research is significant
Socrative

11. Significance
This study is significant because …

12. Significance
cell division is critical to many essential processes of multicellular organisms:
growth & development, reproduction
important to have a basic understanding of the process and of the regulation to ensure accuracy:
deformity, cancer
new information may contribute to a better understanding of human health and disease

13. Significance
cell division is critical to many essential processes of multicellular organisms:
growth & development, reproduction
important to have a basic understanding of the process and of the regulation to ensure accuracy:
deformity, cancer
new information may contribute to a better understanding of human health and disease
this study can contribute to our understanding of molecular interactions essential for accurate cell division by identifying and describing novel components of the kinetochore apparatus
Be sure that your description of the significance includes both broad and specific implications of the work

14. Figure Legends Type of info Type of assay or experiment
Results or conclusion

15. …can show us trends and relationships.
Figures
…can show us trends and relationships.

16. Trends or Relationship
Data:
photos of protein gels
sequence alignment diagram
pictures of cells
The relationships illustrated were:
interaction w/ ZW10/ROD
colocalization
correlation of their activities
Supporting evidence:
Evolutionary relationship, based on sequence and similarity of localization & fxn in human cells

17. Other elements Design of experiment Funding Control(s)
Variables/Treatments
Funding