1. Flower Forensics
Should be on screen as participants enter the room. Start workshop on time—do not wait for “stragglers” Welcome participants as they enter room Do NOT do participant introductions unless the workshop group is a very small one (less than 10 people). Introduce self and briefly explain teaching experience and current position
The activity that you will do today was developed by the Life Sciences Learning Center at the University of Rochester. We found that teachers wanted access to the activities that we developed even after our grant support had ended.
To provide for sustained dissemination, we formed a small spin-off company called Science Take-Out. Explain that Science Take-Out is a small company that manufactures and sells hands-on science kits for middle or high school biology students.
Explain that Science Take-Out is a small company that manufactures and sells hands-on science kits for middle or high school biology students

2. Please complete the “Participant Card”
Because this workshop is supported through a grant from the National Institutes of Health, it is important that we collect data from workshop participants.
Please fill in the participant card during the workshop.
Use the space at the bottom and the back of the card to provide comments on the workshop or the kits used during the workshop. 2 2

3. Envision classroom use
Put your student hat on
Experience the kit
Put your teacher hat on
Envision classroom use
Curriculum integration
Support for students
When we do hands-on workshops we ask you to switch between two hats as you work—your student hat and teacher hat.
We want to you work with partners to experience the kit by completing the activity
We encourage you also have conversations about how you might integrate this into your curriculum and/or support your students.
If you have questions, please call me over while you are working on the activity.
Also, you may find me interrupting to provide further explanation as you work.

4. Flower Forensics Student Handouts
Quick Guide
Safety
Student Instructions
DISTRIBUTE Flower Forensics KITS—ONE kit, one student handout, and one bag of cut-outs PER PAIR OF TEACHERS
For today, you will be working in teams of 2. Kits typically come with ONE student handout in the bag. One teacher should use the student handout that comes in the kit. The other teacher should use the extra copy at the desk.
Teachers may make additional copies if their students are working in teams.
Notice that each kit contains safety instructions and also a graphic quick guide. Students can refer to the quick guide to identify the materials they will need. We do not make a materials list. Instead, students are encouraged to get what they need as they work.
Explain that I’ve used this activity as a “laboratory practical” or “substitute lesson plan” or “just before vacation filler”
Some teachers have enlarged and laminated the quick guides to make “placemats” to help students organize kit materials.
Explain that the labeling of droppers and tubes allows for reuse—a significant cost savings. Droppers can be rinsed out, or just squirted out. Tubes can be refilled.
Turn to this page

5. The owner of a local flower store (Orchid World) has asked you to investigate a possible crime – the theft and illegal cloning of some of his prized black orchids. He has spent many years creating a new strain of rare black orchid that is resistant to insect predators. He recently noticed that three online flower companies are selling black orchids that seem similar to his. He is suspicious that these companies have stolen and are cloning some of his orchids.
Ask someone to read aloud.
Do you think that the orchids are illegal clones of the Old World Orchid???
How could you tell for sure.

6. Your task: Analyze the evidence to determine if the orchids sold by the online flower companies are clones of the Orchid World company’s black orchids.
Paraphrase—What are you going to do with this lab activity?
Your Task: Analyze biological evidence to determine if the orchids sold by the online flower companies (orchid A, orchid B, and orchid C) are clones of (identical to) the Orchid World company’s black orchid.

7. Orchid
Part 1
Flower Structure
Part 2
Paper Chromatography
Part 3
Test for
Chemical X
(fizzes or no reaction)
Part 4
Gel Electrophoresis DNA Banding Pattern
Part 5
Protein Amino Acid Sequences
World
Online Orchid A B C
There is a data table that students will use to record their data on the last page of the student handout.
Having a data table that is separate allows students to avoid a lot of page flipping to record data.
If you want to, you may ask the students to design their own data table.

8. Part 1: Analysis of Flower Structure
You will compare the structure of the Orchid World orchid with the orchids sold by the online flower company.
Ask participants to complete Part 1: Analysis of Flower Structure

9. Part 2: Analysis of Flower Pigments
You will use paper chromatography to compare the pigments present in the four orchid flowers. Chromatography is a method used to separate the plant pigments into a distinct banding pattern of different colors.
Your kit contains droppers of the orchid flower extracts that were created by grinding up the orchid flowers. Follow the paper chromatography procedure below compare the pigments of your four orchid samples.
Ask participants to complete Part 2: Analysis of Flower Pigments

10.  Part 3: Test for Chemical X
Orchid World’s black orchids produce a special chemical (Chemical X) that protects the flowers from being eaten by insect predators. You will test the orchid flower extracts for the presence of Chemical X by mixing a small amount of the extracts with Chemical X Indicator Powder. A fizzing reaction will occur if Chemical X is present in the orchid extracts.
Ask participants to complete Part 3: Test for Chemical X

11. Part 4: Compare the DNA from the Orchid Plants
Cloned plants are produced by asexual reproduction. If the online orchids were cloned from the Orchid World black orchid, they should be genetically identical to the original plant. This means that the coded information in DNA molecules of cloned plants should be identical To the Orchid World orchid.
You can use DNA samples from the four plants (OW, A, B, and C) to make “DNA fingerprints” for the plants.
Ask participants to complete Part 4: Compare the DNA from the Orchid Plants

12. Part 5: Comparing the Protein Amino Acid Sequences from the Orchids
The genetic information stored in DNA is used to assemble amino acids into protein chains. Cloned plants should produce identical proteins. The final test that you will do is to compare the sequence of amino acids in the proteins produced by the flower color gene in the four orchids.
Ask participants to complete Part 5: Comparing the Protein Amino Acid Sequences from the Orchids

13. Part 6: Data Analysis
You will now analyze ALL of the data that you have recorded on the data table. You may find it helpful to highlight or circle the characteristics that that orchids A, B and C have in common with the Orchid World orchid.
Which of the orchids (Orchid A, B or C) are likely to be clones of the Orchid World orchid? Why or who not? Support your answer by citing specific evidence from your data analysis.
Ask participants to complete Part 6: Data Analysis
If time permits, ask several teachers to read their answers to the questions. If you do this, put the previous slide with the data table on for reference as teachers read their answers.

14. Orchid Explain that the Teacher Information contains an answer key.
Part 1
Flower Structure
Part 2
Paper Chromatography
Part 3
Test for
Chemical X
(fizzes or no reaction)
Part 4
Gel Electrophoresis DNA Banding Pattern
Part 5
Protein Amino Acid Sequences
World
Black
Blue
Yellow
fizzes
4 bands
6,8,10,13
Online Orchid A
Smaller and throat shape is different
Pink or red
3 bands
8,13,16
One difference
Pro instead of Leu B
Smaller but structures are the same
No difference C
Wider but structures are the same
No reaction
(DOES NOT fizz)
Ile instead of Leu
Explain that the Teacher Information contains an answer key.

15. Teacher Information MSDS Key Safety Quick Guide
One copy of the Teacher Information is provided when you purchase kits. You can also download the teacher information (without answers) from the website.
The teacher information includes: Summary, Core Concepts, Kit Contains, Teacher Provides, Time Required, Hints for Reusing, and information of refill kit contents.
We also sell refill kits so that basic kit contents can be reused.
MSDS
Key
Safety
Quick Guide

16. www.sciencetakeout.com Purchase kits from
The kit you used today is available from Science Take-Out as a completely assembled student kit.
Please explore the website to learn about other STO kits.
You can download the complete teacher instructions so that you can see what you are buying.

17. Individual Assembled Kits Fully assembled individual kits
You have a brochure that includes information on other Science Take-Out kits and a price list. Visit the Science Take-Out website to get further information for each kit.
At the website, you can download the teacher information for each kit to help you decide which kits you would like to purchase.
Science Take-Out kits are available as:
Individual Assembled Kits like the kits you used in this workshop
Unassembled Packs that contain all supplies needed to make 10 kits
Refill Packs that contain supplies needed to refill the consumables for 10 kits
Individual Assembled Kits Fully assembled individual kits
Unassembled Packs All supplies needed to make 10 kits
Refill Packs All supplies needed to refill 10 kits

18. Help us make new Science Take-Out kits teacher and student friendly.
Become Involved
as a Field Test Teacher
Help us make new Science Take-Out kits teacher and student friendly.
Indicate this on your card. Science Take-Out will contact you with further information
Science Take-Out has received an NIH Small Business grant. Support from this grant will allow us to field test Science Take-Out kits
If you would like to be a field test teacher, indicate this by circling yes on your participant card. We will add you to our list for field test teacher recruitment. 18 18

19. Become Involved as a Workshop Presenter
Present a workshop to introduce colleagues to Science Take-Out kits
Visit the Science Take-Out website for further information
Science Take-Out has received an NIH Small Business grant. Support from this grant will allow us to field test Science Take-Out kits
If you would like to be a field test teacher, indicate this by circling yes on your participant card. We will add you to our list for field test teacher recruitment. 19 19

20. Thanks for being a GREAT group!!!
Please turn in your participant card
before you leave.
Encourage teachers to write comments on the back of the participant survey card.
Please remember to collect participant feedback cards!!