1. TOPIC: Microscope AIM: How do we use a microscope?

2. Eyepiece lens magnification x objective lens magnification
Total
magnification

3. Total magnification=4 x10= 40

4. 100x
400x

5. Rules
when
using a
microscope
1. Carrying microscope =
1 hand on arm, other hand under base close to body

6. 2. DO NOT use coarse adjustment under high power
bc it can break the lens or the slide

7. 3. Always start off with low power bc it gives you a greater field of view.
HIGH

8. 4. Before switching to high power you must:
a. focus your image
b. center your image

9. 5. Adjust the diaphragm
(When switching from low to high, amount of light decreases)

10. 6. Use LENS PAPER to clean lens

11. Treating specimen with solutions of different colors
What is
staining?
Treating specimen with solutions of different colors
Make cell parts more visible

12. Unstained cheek cells
Stained cheek cells

13. Examples:
1. Lugol’s Iodine
(plant cells)

14. Onion Cells stained with Lugol’s Iodine

15. 2. Methylene blue
(animal cells)

16. Cheek Cells
Unstained
Stained

17. Skin Cells stained with Methylene Blue

18. Wet
mount
1. Place a drop of water on the slide

19. 2. Place object on the slide.

20. 3. Slowly lower cover slip onto specimen on an angle to avoid AIR BUBBLES.

23. 4. Absorbed excess water with a paper towel.

25. F Upside down & backwards How do images appear? On the slide
Seen through lens F

26. Image moves in opposite direction
What
happens
when
you
move
the
slide?