1. C. 24-well whole blood (HIV+)
A. 96-well whole blood
B. 96-well PBMC
C. 24-well whole blood (HIV+)
CD4
0.00
0.25
0.50
0.75
1.00
1.25
CD8
0.0
0.5
1.0
1.5
2.0
2.5 1 2 3 4
CD4 1 2 3 4
CD8 1 2 3
CD8
Peptide
mix
% IFNg+ cells r 2
= 0.95 r 2
=0.87 r 2
= 0.96 r 2
= 0.73 r 2 = . 7 3
p<0.0001
p<0.0001
p <
p = p
< . 2
slope 0.94
slope 0.88
slope = 1.2
slope = 1.1 s l o p e = 7 4
plate
0.0
2.5
5.0
7.5
10.0 2 4 6 8 10 12 14 10 20 10 20 30 5 1
SEB
% IFNg+ cells 2 r 2 2
=0.95 r 2
= 0.74 r
= 0.79 r 2 = . 6 7 r
= 0.94
p<0.0001
p =
p < p
< . 4
p<0.0001
slope 0.93
slope = 0.78
slope = 0.84 s l o p e = . 7
slope 0.91
% IFNg+ cells
% IFNg+ cells
% IFNg+ cells
% IFNg+ cells
% IFNg+ cells
tube