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Side Scatter Forward Scatter Lineage CD1c CD141 CD303 lineage negative

Published bySylvia Morgan Modified over 6 years ago

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Presentation on theme: "Side Scatter Forward Scatter Lineage CD1c CD141 CD303 lineage negative"— Presentation transcript:

1 Side Scatter Forward Scatter Lineage CD1c CD141 CD303 lineage negative CD1c+ mDC CD141+ mDC CD303+ pDC DC SUBSETS A HLA-DR total DC TOTAL DC B CD4+ lymphocytes total Tregs CD25 CD127 CD45RA CD4 TREG CELLS C active Tregs resting Tregs copy gate Additional figure 1 Representative staining of DC and T cells in fresh whole blood from adults. (A) DC subsets were identified by gating on lineage negative WBC (CD3, 14, 19 and 56) followed by expression of CD303, CD1c and CD141 surface markers to identify CD303+ plasmacytoid DC, CD1c+ myeloid DC and CD141+ myeloid DC, respectively. (B) Total DC were identified as lineage negative (CD3, 14, 19, 34 and 56) and HLA-DR positive PBMC. (C) Total Treg cells were identified as CD4+ lymphocytes with high expression of CD25 and low expression of CD127 surface markers. Total Treg cells were then subdivided into active and resting Treg cells based on expression of CD45RA. Abbreviations: WBC, white blood cells; PBMC, peripheral blood mononuclear cells; DC, dendritic cells; Treg, regulatory T cells.

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