1. Medical Internship Summary
Jane Smith
Neonatology Research
Dr. Smith and Dr. John
University of Rochester Medical Center (URMC)
Pediatrics Department
Organization Description
URMC strives to develop a high-quality, cost-effective, patient-centered and innovative academic healthcare system led by our world class researchers, educators, and clinicians. Dr. Smith and Dr. John are two research scientists within the department of Pediatrics and Neonatology. Currently working on a grant about gene therapy, they are working together to collect data as well as find cures and treatments for a variety of diseases to help the community.
Objectives
Obtain hands-on experience and more knowledge about biology
Focus on what career I would like to pursue in the wide range of biology related careers
Participate in research lab work
Learn how to properly create experiments
Gain knowledge on cloning DNA and plasmids
Extract and correctly analyze data
Activities
Cloned DNA by using a Polymerase Chain Reaction
Made Agarose gel, loaded DNA and ran a gel electrophoresis
Extracted and Purified DNA
Performed a recombination assay
Grew and plated bacteria on Kanamycin plates
Electroporated bacteria for gene entry
Digested samples using restriction enzymes
Used an autoclave to properly sterilize both dry and wet materials
This valuable experience reaffirmed my interest in the medical field and solidified my thoughts about working in research concentrated at the cellular level. Furthermore, this has helped me apply my coursework to a real work environment in order to make effective career choices. I am looking forward to working in a lab in the future and continue to expand my knowledge of molecular biology.