1. Algorithmic Approach to Diagnosis of Hydatidiform Moles
Possible Hydatidiform Mole
p57 immunohistochemistry
Negative p57
(villous stroma, cytotrophoblast)
Positive p57
(villous stroma, cytotrophoblast)
Morphology appropriate
Morphology equivocal
Molecular genotyping
Androgenetic diploidy
Diandric triploidy
Biparental diploidy
CHM or eCHM
PHM
Non-molar

2. Supplemental figure legend: Algorithmic approach to diagnosis of hydatidiform moles. Potential molar cases are first triaged with p57 immunohistochemistry. If p57 is negative and morphology is appropriate, a diagnosis of complete hydatidiform mole (CHM) or early complete hydatidiform mole (eCHM) can be rendered. If p57 is positive, molecular genotyping is performed (regardless of morphology). The subtypes of molar specimens, including CHMs and partial hydatidiform moles (PHM) are distinguished from non-molar specimens by the specific genotyping results indicated.