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Published byCordelia Juliet Patterson Modified over 6 years ago
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Affinity Chromatography By: Ayesha Naeem
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Chromatography Chromatography is the term of collection of lab techniques for separation of mixtures Derived word Chroma : color Graphein : write
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Essential parts of chromatography
STATIONARY PHASE: Supports the sample that is to be tested. MOBILE PHASE: That moves in even direction. SAMPLE : That is to be tested.
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TYPES OF CHROMATOGRAPHY
AFFINITY ION EXCHANGE PARTITION ADSORPTION SIZE EXCLUSION
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Here We’ll discuss; AFFINITY CHROMATOGRAPHY
Diverse method for the purification of biomolecules and specific molecules from complex compounds Most specifically ; Proteins
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Important 2 terms relevant to Affinity Chromatography
Matrix Spacer arm Ligand
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Examples of affinity chromatography
Antigen-antibodies Substrate-enzyme DNA-histon Hormone-receptors
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Working Principle Step 1 Step 2 Step 3
Attachment of ligand to column matrix. Step 1 Load mixture into column. Step 2 Binding of specific molecules to the ligands. Step 3
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Merci bien
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