1. MPP-96 — 96 Well Plate Photometer for ELISA

2. Structure of Presentation
Introduction
Specification
Software
Clinical Trials
Discussion

3. Introduction
ELISA is a popular format of analytic biochemistry assay that uses a solid-phase enzyme immunoassay to detect the presence of a substance, usually an antigen, in a liquid or wet sample.

4. ELISA types Indirect ELISA Sandwich ELISA Competitive ELISA
Multiple and portable ELISA

5. Readers

6. Specification: General
Detection mode
Absorbance
Light source
LED, self-calibrating
Photodetector
Silicon photodiodes
Plate type
96-well microplates including strip-well
microplates)
Reading Speed
~5 s per wavelength
Measurement channels
8 (plus reference)
Measurement range
0 – 4.3 OD
Resolution
0.001 OD
Wavelength range
400 – 700 nm
Wavelength selection up to 8* filters on wheel standard filters 405, 450, 492 and 620 nm
Shaking
Linear, 4 amplitudes, 4 speeds
Software
QuantAssay

7. Accuracy, Precision
Accuracy 450 / 492 nm
0.000 – OD
≤ (0.5 % ± OD) typical
2.000 – OD
≤ (1 % ± OD) typical
3.000 – OD
≤ (2 % ± OD) typical
Precision 450 / 492 nm
≤ (0.5 %)
2.000 – OD
≤ (1.0 %)
As measurand for verification we use Multiskan Verification Plate (Cat.# BS AK)

8. Software: Specifications
Basis
To fit with Vector-Best technical specification
Reliable operation of the program:
Ease of adding samples, editing assays and data analysis
Advanced assay editor
Save, load, export results
Multi-language

9. Conception Assay selection Sample filling Measurement
Validation of results
Results Export

10. QuantAssay Features
ELISa assays of any complexity can be carried out via robust Assay Editor
Multiplex assays-up to 7 assays on one plate
Qualitative assay includes up to 8 controls
Quantitative assay includes up to 20 standards
BestFit function for selecting the best calibration curve
Save, load and export results
Creating visual reports

11. Assay Editor
Qualitative
Quantitative Linear/Reverse
Avidity
Multiplex

12. Qualitative Yes/No or Positivity factor assays
If Test samples > Critical OD, then Result = Positive

13. Positivity factor

14. Quantitative Linear
Linear — with concentration -- OD is growing

15. Quantitative Reverse
Reverse — with concentration OD is descending

16. Avidity
Dissociating agent
High avidity
Low avidity

17. Multiplex assays

18. Clinical trials results
Readers: HiPo (Biosan) vs ELx 800 (Biotek)
Softwares: QuantAssay (Biosan) vs GEN5 (Biotek)
Using Interleukin-8 Quantitative detection kit from Vector-Best

19. QuantAssay and GEM5 calibration curves

20. QuantAssay and GEM5 Optical Density comparison
The Hipo reader and QuantAssay software provide same results as ELx800 reader and GEM5 software

21. BIOSAN ELISA Line
UNDER DEVELOPMENT

22. Thank you
Questions, please.