1. The Suspects Will, Captain of the Football Team Liza, Head Cheerleader
Jude,
Senior Class
President
Natalie,
Valedictorian
Molly,
Rival High
Homecoming
Queen
Maggie,
Lead actress
in school play
Vince,
Drum Major

2. Restriction Enzyme Digestion Analysis
EcoRI cuts the following sequence:
G|AATTC
Find all EcoRI cut sites in the DNA sequence of the suspect you represent
Draw the bands on the gel according to their predicted lengths following digestion
Can we ID the thief??

3. TCGATGAATTCTATCGGAATTCTCGGACTTCTCGAGAATTCTGCGGATTTCTCGGATTCA
Suspect #1: Will
DOB: 07/11/1989
Sex: Male
Weight: 220 lb.
Height: 6’1”
Position: Captain of the Football Team
DNA Sequence:
TCGATGAATTCTATCGGAATTCTCGGACTTCTCGAGAATTCTGCGGATTTCTCGGATTCA
DNA Fragment Sizes: _________________ 1 35 30 28 24 20 16 10 12 5 M
Thief
EcoRI: G|AATTC

4. TCGATGAATTCTATCGCAATTCTCGCAATTCTCGAGAATTCTGCGGATTTCTCGGATTCA
Suspect #2: Natalie
DOB: 06/19/1990
Sex: Female
Weight: 125 lb.
Height: 5’6”
Position: Class of 2007 Valedictorian
DNA Sequence:
TCGATGAATTCTATCGCAATTCTCGCAATTCTCGAGAATTCTGCGGATTTCTCGGATTCA
DNA Fragment Sizes: _________________ 1 35 30 28 24 20 16 10 12 5 M
Thief
EcoRI: G|AATTC

5. TCGATGAAGTCTATCGGAATTCTCGGAATTCTCGACAATTCTGCGGAATTCTCGGATTCA
Suspect #3: Molly
DOB: 09/15/1989
Sex: Female
Weight: 145 lb.
Height: 5’11”
Position: Rival High Homecoming Queen
DNA Sequence:
TCGATGAAGTCTATCGGAATTCTCGGAATTCTCGACAATTCTGCGGAATTCTCGGATTCA
DNA Fragment Sizes: _________________ 1 35 30 28 24 20 16 10 12 5 M
Thief
EcoRI: G|AATTC

6. TCGATCAATTCTATCGGAATTCTCGGATTTCTCGACAATTCTGCGGAATTCTCGGATTCA
Suspect #4: Jude
DOB: 03/14/1989
Sex: Male
Weight: 190 lb.
Height: 5’10”
Position: Senior Class President
DNA Sequence:
TCGATCAATTCTATCGGAATTCTCGGATTTCTCGACAATTCTGCGGAATTCTCGGATTCA
DNA Fragment Sizes: _________________ 1 35 30 28 24 20 16 10 12 5 M
Thief
EcoRI: G|AATTC

7. TCGATGAATTCTATCGGAATTCTCGGAATTCTCGACAATTCTGCGGAATTCTCGGATTCA
Suspect #5: Maggie
DOB: 05/03/1990
Sex: Female
Weight: 140 lb.
Height: 5’8”
Position: Lead actress in school play
DNA Sequence:
TCGATGAATTCTATCGGAATTCTCGGAATTCTCGACAATTCTGCGGAATTCTCGGATTCA
DNA Fragment Sizes: _________________ 1 35 30 28 24 20 16 10 12 5 M
Thief
EcoRI: G|AATTC

8. TCGATGAATTCTATCGAAATTCTCGGAATTCTCGAGAATCCTGCGGACTTCTCGGATTCA
Suspect #6: Vince
DOB: 08/11/1989
Sex: Male
Weight: 210 lb.
Height: 6’2”
Position: Drum Major
DNA Sequence:
TCGATGAATTCTATCGAAATTCTCGGAATTCTCGAGAATCCTGCGGACTTCTCGGATTCA
DNA Fragment Sizes: _________________ 1 35 30 28 24 20 16 10 12 5 M
Thief
EcoRI: G|AATTC

9. TCGATGAACTCTATCGGAATTTTCGGAATTCTCGAGATTTCTGCGGAATTCTCGGATTCA
Suspect #7: Liza
DOB: 02/28/1989
Sex: Female
Weight: 110 lb.
Height: 5’3”
Position: Head Cheerleader R
DNA Sequence:
TCGATGAACTCTATCGGAATTTTCGGAATTCTCGAGATTTCTGCGGAATTCTCGGATTCA
DNA Fragment Sizes: _________________ 1 35 30 28 24 20 16 10 12 5 M
Thief
EcoRI: G|AATTC

10. Who done it?

11. 35 30 28 24 20 16 12 10 5 1 Crime Scene DNA Suspect #2: Natalie
Suspect #1: Will
Suspect #3: Molly
Suspect #4: Jude
Suspect #5: Maggie
Suspect #6: Vince
Suspect #7: Liza
DNA Ladder 35 30 28 24 20 16
****If you don’t have a preprinted poster of a blank gel, make sure you draw a gel on the board for them to fill in if you are using powerpoint. Alternatively, use the blank gel on this slide printed on an overhead *****
Pass out a suspect sheet to each student.
Have each student go through and find all the EcoRI cut sites, determine the size of each fragment, and draw the DNA pieces on the gel.
Have the students with the same suspect get together and compare their EcoRI digestion analysis.
Have one representative from each group go to the board and drawn their digestion pattern on the gel.
Identify the thief as a class. 12 10 5 1