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Two Dimensional Gel Electrophoresis (2DE) of extracellular protein of Talaromyces verruculosus SGMNPf3 (a) Standard (b) sample study.

Published byOswin Sherman Modified over 6 years ago

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Presentation on theme: "Two Dimensional Gel Electrophoresis (2DE) of extracellular protein of Talaromyces verruculosus SGMNPf3 (a) Standard (b) sample study."— Presentation transcript:

1 Two Dimensional Gel Electrophoresis (2DE) of extracellular protein of Talaromyces verruculosus SGMNPf3 (a) Standard (b) sample study

2 Uniprot/NCBI identifier (protein name/species)
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 1 A1DCV5/ Probable beta-glucosidase L (EC: ) (Neosartorya fischeri ) 152.93 8 7 LC-MS/MS 2 A1DCV5/331117 136.73 3 Q8WZJ4/28572 1,4-beta-D-glucan cellobiohydrolase Penicillium funiculosum 266.34 43 39 4 213.11 33 5 (Penicillium funiculosum) 231.56 46 31 6 290.71 50 63 227.49 26 208.23 14 9 Q5ZNB1/28572 Endo-1,4-beta-xylanase D (EC: ) ( Penicillium funiculosum) 172.60 10 180.74 21 11 321.83 12 390.94 55 36 13 237.42 35 15 155.84 131.91 16 124.72 17 gi/ Cellobiohydrolase precursor 109 MALDI-TOF MS/MS

3 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 1 A1DCV5/ Probable beta-glucosidase L (EC: ) (Neosartorya fischeri ) 152.93 8 7 LC-MS/MS

4 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 2 A1DCV5/331117 Probable beta-glucosidase L (EC: ) (Neosartorya fischeri ) 136.73 7 8 LC-MS/MS

5 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 3 Q8WZJ4/28572 1,4-beta-D-glucan cellobiohydrolase Penicillium funiculosum 266.34 43 39 LC-MS/MS

6 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 4 Q8WZJ4/28572 1,4-beta-D-glucan cellobiohydrolase Penicillium funiculosum 213.11 33 17 LC-MS/MS

7 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 5 Q8WZJ4/28572 1,4-beta-D-glucan cellobiohydrolase (Penicillium funiculosum) 231.56 46 31 LC-MS/MS

8 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 6 Q8WZJ4/28572 1,4-beta-D-glucan cellobiohydrolase (Penicillium funiculosum) 290.71 50 63 LC-MS/MS

9 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 7 Q8WZJ4/28572 1,4-beta-D-glucan cellobiohydrolase (Penicillium funiculosum) 227.49 26 LC-MS/MS

10 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 8 Q8WZJ4/28572 1,4-beta-D-glucan cellobiohydrolase (Penicillium funiculosum) 208.23 14 4 LC-MS/MS

11 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 9 Q5ZNB1/28572 Endo-1,4-beta-xylanase D (EC: ) ( Penicillium funiculosum) 172.60 26 5 LC-MS/MS

12 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 10 Q8WZJ4/28572 1,4-beta-D-glucan cellobiohydrolase (Penicillium funiculosum) 180.74 33 21 LC-MS/MS

13 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 11 Q5ZNB1/28572 Endo-1,4-beta-xylanase D (EC: ) ( Penicillium funiculosum) 321.83 26 6 LC-MS/MS

14 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 12 Q5ZNB1/28572 Endo-1,4-beta-xylanase D (EC: ) ( Penicillium funiculosum) 390.94 55 36 LC-MS/MS

15 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 13 Q5ZNB1/28572 Endo-1,4-beta-xylanase D (EC: ) ( Penicillium funiculosum) 237.42 35 15 LC-MS/MS

16 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 14 Q5ZNB1/28572 Endo-1,4-beta-xylanase D (EC: ) ( Penicillium funiculosum) 155.84 21 4 LC-MS/MS

17 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 15 Q8WZJ4/28572 1,4-beta-D-glucan cellobiohydrolase (Penicillium funiculosum) 131.91 11 4 LC-MS/MS

18 Based on Homology search
2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 16 Q8WZJ4/28572 1,4-beta-D-glucan cellobiohydrolase (Penicillium funiculosum) 124.72 12 4 LC-MS/MS

19 Based on Homology search
MALDI Spectra Based on Homology search 2D gel Spot No. Uniprot/NCBI identifier Description (protein name/species) Mascot score % Sequence coverage Peptides matched MS system used for identification 17 gi/ Cellobiohydrolase precursor 109 1 MALDI-TOF MS/MS

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