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2. Standardized Test Prep
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Standardized Test Prep

3. Chapter 23 Table of Contents Section 1 Prokaryotes
Bacteria
Table of Contents
Section 1 Prokaryotes
Section 2 Biology of Prokaryotes
Section 3 Bacteria and Humans

4. Section 1 Prokaryotes
Chapter 23
Objectives
Explain the phylogenetic relationships between the domains Archaea, Bacteria, and Eukarya.
Identify three habitats of archaea.
Describe the common methods used to identify bacteria.
Identify five groups of bacteria.
Explain the importance of nitrogen-fixing bacteria for many of Earth’s ecosystem.

5. Two Major Domains: Archaea and Bacteria
Section 1 Prokaryotes
Chapter 23
Two Major Domains: Archaea and Bacteria
Prokaryotes are single-celled organisms that do not have a membrane-bound nucleus, and can live in nearly every environment on Earth.
Although tiny, prokaryotes differ greatly in their genetic traits, their modes of nutrition, and their habitats.
Based on genetic differences, prokaryotes are grouped in two domains: Domain Archaea and Domain Bacteria.

6. Three Domains of Living Organisms
Section 1 Prokaryotes
Chapter 23
Three Domains of Living Organisms

7. Chapter 23 Domain Archaea
Section 1 Prokaryotes
Chapter 23
Domain Archaea
One of the ways in which archaea differ is the make up of their cell wall. Archaeal cells walls do not contain peptidoglycan.
Peptidoglycan is a protein-carbohydrate complex found in bacterial cell walls that make their cells walls rigid.
Archaea differ in the types of lipids in their cell membrane. Also, archaeal genes do contain introns.

8. Domain Archaea, continued
Section 1 Prokaryotes
Chapter 23
Domain Archaea, continued
Archaeal Groups…
…include methanogens, halophiles, and thermoacidophiles.
Methanogens convert hydrogen gas and carbon dioxide into methane. They can be found in the intestines of organisms such as cattle and termites
Responsible for “swamp gas” and methane belched by cows
Live only in anaerobic environment (no oxygen).

9. Domain Archaea, continued
Section 1 Prokaryotes
Chapter 23
Domain Archaea, continued
Archaeal Groups, continued
Halophiles are “salt-loving” archaea that live in very salty environments such as the Great Salt Lake and the Dead Sea.
Thermoacidophiles live in very hot, acidic environments, such as the hot springs of Yellowstone National Park. Some live at temperatures up to 110°C (230°F) and at a pH of less than 2.
The base of food webs in many communities

10. Chapter 23 Domain Bacteria
Section 1 Prokaryotes
Chapter 23
Domain Bacteria
Bacteria occur in many shapes and sizes. Most bacteria have one of three basic shapes: rod-shaped, sphere-shaped, or spiral-shaped.
Rod-shaped bacteria are called bacilli (singular, bacillus). An example of bacilli is Escherichia coli.
Sphere-shaped bacteria are called cocci (singular, coccus). An example of cocci is Micrococcus luteus.

11. Domain Bacteria, continued
Section 1 Prokaryotes
Chapter 23
Domain Bacteria, continued
Spiral shaped bacteria are called spirilla (singular, spirillum). An example of spirilla bacteria includes Spirillum volutans.
Cocci that form chains similar to a string of beads are called streptococci.
Cocci that form clusters similar to a bunch of grapes are called staphylococci.

12. Three Bacterial Cell Shapes
Section 1 Prokaryotes
Chapter 23
Three Bacterial Cell Shapes

13. Characteristics of Bacteria
Section 1 Prokaryotes
Chapter 23
Characteristics of Bacteria
Click below to watch the Visual Concept.
Visual Concept

14. Domain Bacteria, continued
Section 1 Prokaryotes
Chapter 23
Domain Bacteria, continued
Gram Stain
Most species of bacteria are classified into two categories based on the structure of their cell walls as determined by a technique called the Gram stain.
Gram-positive bacteria have a thick layer of peptidoglycan in their cell wall, and they appear purple under a microscope after the Gram-staining procedure.
Gram-negative bacteria have a thin layer of peptidoglycan in their cell wall, and they appear reddish-pink under a microscope after the Gram-staining procedure.

15. Section 1 Prokaryotes
Chapter 23
Gram Staining

16. Chapter 23 Gram Stain Section 1 Prokaryotes
Click below to watch the Visual Concept.
Visual Concept

17. Important Bacterial Groups
Section 1 Prokaryotes
Chapter 23
Important Bacterial Groups
Bacteria are also classified by their biochemical properties and evolutionary relationships.
Proteobacteria
Proteobacteria are one of the largest and most diverse groups of bacteria, and contain several subgroups that are extremely diverse.
Members of this group include bacteria of the genus Rhizobium, the genus Agrobacterium, and the bacterium Escherichia coli.

18. Important Bacterial Groups, continued
Section 1 Prokaryotes
Chapter 23
Important Bacterial Groups, continued
Gram-Positive Bacteria
Not all of the bacteria in this group are Gram-positive. Biologists place a few species of Gram-negative bacteria in this group because these species are genetically similar to Gram-positive bacteria.
Members of this group include the streptococcal species, Clostridium botulinum, Bacillus anthracis, and members of the genus Mycobacteria.

19. Important Bacterial Groups, continued
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Important Bacterial Groups, continued
Gram-Positive Bacteria, continued
Actinomycetes are Gram-positive bacteria, some species of which produce antibiotics.
Antibiotics are chemicals that inhibit the growth of or kill other microorganisms. Streptomycin and tetracycline are examples of antibiotics that are used medicinally.

20. Important Bacterial Groups, continued
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Chapter 23
Important Bacterial Groups, continued
Cyanobacteria
Cyanobacteria use photosynthesis to get energy from sunlight, and make carbohydrates from water and carbon dioxide. During this process, they create oxygen as a waste product.
Once called blue-green algae, cyanobacteria are now known to be bacteria because they lack a membrane-bound nucleus and chloroplasts.

21. Important Bacterial Groups, continued
Section 1 Prokaryotes
Chapter 23
Important Bacterial Groups, continued
Spirochetes
Spirochetes are Gram-negative, spiral-shaped bacteria that move by means of a corkscrew-like rotation. Some are aerobic.
Spirochetes can live freely or as pathogens. Pathogenic spirochetes include Treponema pallidum, the causative agent of syphilis, and Borrelia burgdorferi, which causes Lyme disease.

22. Important Bacterial Groups, continued
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Chapter 23
Important Bacterial Groups, continued
Chlamydia
Gram-negative coccoid pathogens of the group Chlamydia live only inside animal cells. The cell walls of chlamydia do not have peptidoglycan. Chlamydia trachomatis causes the sexually transmitted infection called chlamydia.

23. Section 2 Biology of Prokaryotes
Chapter 23
Objectives
Describe the internal and external structure of prokaryotic cells.
Identify the need for endospores.
Compare four ways in which prokaryotes get energy and carbon.
Identify the different types of environments in which prokaryotes can live.
List three types of genetic recombination that prokaryotes use.

24. Structure and Function
Section 2 Biology of Prokaryotes
Chapter 23
Structure and Function
The major structures of a prokaryotic cell include a cell wall, a cell membrane, cytoplasm, ribosomes, and sometimes a capsule, pili, endospores, and flagella.
Cell Wall
Most prokaryotes have a cell wall. Bacterial cell walls contain peptidoglycan. Archaeal cell walls do not have peptidoglycan; instead, some contain pseudomurein, a compound made of unusual lipids and amino acids.

25. Structure and Function, continued
Section 2 Biology of Prokaryotes
Chapter 23
Structure and Function, continued
Cell Membrane and Cytoplasm
Bacterial and archaeal cell membranes are lipid bilayers that have proteins. However, the lipids and proteins of archaeal cell walls differ from those of bacterial cell walls.
The cytoplasm is a semifluid solution that contains ribosomes, DNA, small organic and inorganic molecules, and ions.

26. Structure and Function, continued
Section 2 Biology of Prokaryotes
Chapter 23
Structure and Function, continued
DNA
Prokaryotic DNA is a single closed loop of double-stranded DNA attached at one point to the cell membrane.
Along with a single main chromosome, some prokaryotes have plasmids, which are small, circular, self-replicating loops of double-stranded DNA.

27. Structure and Function, continued
Section 2 Biology of Prokaryotes
Chapter 23
Structure and Function, continued
Capsules and Pili
Many bacteria have an outer covering of polysaccharides called a capsule that protects the cell against drying, pathogens, or harsh chemicals.
Pili are short, hairlike protein structures on the surface of some bacteria that help bacteria connect to each other and to surfaces, such as those of a host cell.

28. Chapter 23 Bacterial Capsule Section 2 Biology of Prokaryotes
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Visual Concept

29. Chapter 23 Pilus Section 2 Biology of Prokaryotes
Click below to watch the Visual Concept.
Visual Concept

30. Structure and Function, continued
Section 2 Biology of Prokaryotes
Chapter 23
Structure and Function, continued
Endospores
Some Gram-positive bacteria can form a thick-coated, resistant structure called an endospore when environmental conditions become harsh.

31. Structure and Function, continued
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Chapter 23
Structure and Function, continued
Prokaryotic Movement
Many prokaryotes have long flagella that allow the prokaryotes to move toward food sources or away from danger.

32. Structural Characteristics of a Bacterial Cell
Section 2 Biology of Prokaryotes
Chapter 23
Structural Characteristics of a Bacterial Cell

33. Parts of a Prokaryotic Cell
Section 2 Biology of Prokaryotes
Chapter 23
Parts of a Prokaryotic Cell
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Visual Concept

34. Nutrition and Metabolism
Section 2 Biology of Prokaryotes
Chapter 23
Nutrition and Metabolism
Prokaryotes obtain nutrients either from the nonliving environment or by utilizing the products or bodies of living organisms.
Heterotrophs obtain carbon from other organisms.
Autotrophs obtain their carbon from CO2.
Chemotrophs get energy from chemicals in the environment.

35. Chapter 23 Prokaryotic Habitats
Section 2 Biology of Prokaryotes
Chapter 23
Prokaryotic Habitats
Different prokaryotic species live in different environments.
Temperature requirements range from 0°C to 110°C.
Most prokaryotic species grow best at a neutral pH.

36. Reproduction and Recombination
Section 2 Biology of Prokaryotes
Chapter 23
Reproduction and Recombination
Genetic recombination in bacteria can occur by the following three ways:
transformation (taking in DNA from the outside environment)
conjugation (exchanging DNA with other bacteria via pili)
transduction (transmission of bacterial DNA via viruses).

37. Chapter 23 Objectives Section 3 Bacteria and Humans
Describe the ways in which bacteria can cause disease in humans.
Explain how a bacterial population can develop resistance to antibiotics.
Identify reasons for recent increases in the numbers of certain bacterial infectious diseases.
Identify ways of preventing a foodborne illness at home.
List four industrial uses of bacteria.

38. Chapter 23 Bacteria and Health
Section 3 Bacteria and Humans
Chapter 23
Bacteria and Health
Human diseases may result from endotoxins or exotoxins produced by bacteria or from the destruction of body tissues.

39. Bacteria and Health, continued
Section 3 Bacteria and Humans
Chapter 23
Bacteria and Health, continued
Antibiotics and Antibiotic Resistance
A mutation in the DNA of a single bacterium can confer resistance to an antibiotic.
Cells with the mutant gene have a selective advantage when the antibiotic is present.
Mutant cells take over the population when the normal cells die.

40. Bacteria and Health, continued
Section 3 Bacteria and Humans
Chapter 23
Bacteria and Health, continued
Emerging Infectious Diseases Caused by Bacteria
The number of certain bacterial diseases has increased because of the increase in the number of antibiotic resistant bacteria, the movement of people into previously untouched areas, and global travel.

41. Bacteria and Health, continued
Section 3 Bacteria and Humans
Chapter 23
Bacteria and Health, continued
Food Hygiene and Bacteria
Foodborne illnesses can be avoided by selecting, storing, cooking, and handling food properly.
Frequent hand washing in hot, soapy water is also very important.

42. Important Bacterial Diseases
Section 3 Bacteria and Humans
Chapter 23
Important Bacterial Diseases

43. Chapter 23 Bacteria in Industry
Section 3 Bacteria and Humans
Chapter 23
Bacteria in Industry
Many species of bacteria are used to produce and process different foods, to produce industrial chemicals, to mine for minerals, to produce insecticides, and to clean up chemical and oil spills.
Biologists have learned to harness bacteria to recycle compounds in a process called bioremediation, which uses bacteria to break down pollutants.

44. Chapter 23 Bacteria and Food Section 3 Bacteria and Humans
Click below to watch the Visual Concept.
Visual Concept

45. Chapter 23 Multiple Choice
Standardized Test Prep
Multiple Choice
1. How do bacteria produce yogurt from milk?
A. by conjugation
B. by fermentation
C. by nitrogen fixation
D. by aerobic respiration

46. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
1. How do bacteria produce yogurt from milk?
A. by conjugation
B. by fermentation
C. by nitrogen fixation
D. by aerobic respiration

47. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
2. What are rod-shaped bacteria called?
F. cocci
G. bacilli
H. spirilla
J. halophiles

48. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
2. What are rod-shaped bacteria called?
F. cocci
G. bacilli
H. spirilla
J. halophiles

49. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
3. What are thermoacidophiles?
A. bacteria
B. archaea
C. spirochetes
D. cyanobacteria

50. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
3. What are thermoacidophiles?
A. bacteria
B. archaea
C. spirochetes
D. cyanobacteria

51. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
4. Genetic recombination in bacteria can occur during which process?
F. conjugation
G. binary fission
H. heterocyst formation
J. endospore production

52. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
4. Genetic recombination in bacteria can occur during which process?
F. conjugation
G. binary fission
H. heterocyst formation
J. endospore production

53. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
Use the table below to answer questions The table lists the response of a bacterium to several antibiotics.

54. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
5. Which of the following antibiotics killed the bacteria most effectively?
A. penicillin
B. bacitracin
C. tetracycline
D. streptomycin

55. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
5. Which of the following antibiotics killed the bacteria most effectively?
A. penicillin
B. bacitracin
C. tetracycline
D. streptomycin

56. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
6. Which of the following antibiotics had no effect on the bacterium?
F. ampicillin
G. tetracycline
H. streptomycin
J. cephalosporin

57. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
6. Which of the following antibiotics had no effect on the bacterium?
F. ampicillin
G. tetracycline
H. streptomycin
J. cephalosporin

58. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
Complete the following analogy:
7. obligate aerobe : oxygen :: obligate anaerobe :
A. low pH
B. absence of oxygen
C. presence of methane
D. high salt concentration

59. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
Complete the following analogy:
7. obligate aerobe : oxygen :: obligate anaerobe :
A. low pH
B. absence of oxygen
C. presence of methane
D. high salt concentration

60. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
Use the image below to answer questions The table shows different shapes in bacteria.

61. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
8. What shape is represented by organism C?
F. coccus
G. bacillus
H. spirillum
J. filamentous

62. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
8. What shape is represented by organism C?
F. coccus
G. bacillus
H. spirillum
J. filamentous

63. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
9. You would expect the members of the bacterial genus Bacillus to be what type of shape?
A. rod shaped
B. spiral shaped
C. square shaped
D. sphere shaped

64. Multiple Choice, continued
Chapter 23
Standardized Test Prep
Multiple Choice, continued
9. You would expect the members of the bacterial genus Bacillus to be what type of shape?
A. rod shaped
B. spiral shaped
C. square shaped
D. sphere shaped

65. Chapter 23 Short Response
Standardized Test Prep
Short Response
The Gram stain is used to distinguish bacteria based on a certain physiological difference between the bacteria.
Describe the color of Gram-positive and Gram-negative bacteria when they are treated with the Gram stain.

66. Short Response, continued
Chapter 23
Standardized Test Prep
Short Response, continued
The Gram stain is used to distinguish bacteria based on a certain physiological difference between the bacteria.
Describe the color of Gram-positive and Gram-negative bacteria when they are treated with the Gram stain.
Answer: Gram-positive bacteria appear purple. Gram-negative bacteria appear reddish-pink.

67. Chapter 23 Extended Response Standardized Test Prep
Base your answers to parts A & B on the information below.
Species of the symbiotic bacterial genus Rhizobium live within the root nodules of legumes, such as soybeans and peanuts. Some farmers take advantage of the nitrogen-fixing abilities of these species when they rotate their crops every few years and grow legumes instead of their normal food crops.
Part A Define nitrogen fixation.
Part B Explain the benefits of crop rotation on the soil.

68. Extended Response, continued
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Standardized Test Prep
Extended Response, continued
Answer:
Part A Nitrogen fixation is the conversion of nitrogen gas into a form of nitrogen (ammonia) that plants can use.
Part B Crop rotation of legumes with nonlegume crops would add nitrogen to the soil without having to use artificial fertilizers.