1. Innovative Analytical Solutions for the Dairy Industry

2. Bentley CombiFTS
Rapid and accurate determination of milk chemical composition and somatic cells content
Milchprüfring Baden-Württemberg, Germany
5xCombiFTS 500, 1 IBC150

3. Bentley Bartec CombiFTS 600
Up to 600 samples/hr

4. Bentley Bartec CombiFTS 500

5. Bentley ILAS CombiFTS 600
Up to 600 samples/hr

6. Bentley ILAS CombiFTS 600 The Optimum Solution
Fully Automated
Warmed up in a water bath, shaken and identified (RFID, Barcode)
Uncapped
Samples are replaced in the tray after analysis
Pilot samples automatically inserted in the process

7. Bentley CombiFTS
Somacount FCM (Flow Cytometry)
Bentley FTS (FTIR)

8. Bentley FTS Milk Component Absorption Wavebands Lactose (OH)
Protein (NH)
Fat A (C=0)
Fatty Acids
Fat B (CH)
Lactose (OH)
Frequency of infrared radiation absorbed depending upon type of molecular bonds /vibration mode

11. Recordings from the Instrument
Infrared Spectrum
Somatic cells peak distributions
Environmental variables could affect the end results
Sample, water bath, ambient, homogenizer, deck temperatures
Optical deck Moisture level and pressure
Centerburst energy level (automatic alignment)
Homogenizar pressure
Vibrations

12. FTS Infrared Applications
Traditional Components
New Components
New Developments
Blood BHB
Lactoferrin
Iodine Value
Fatty Acids calibrations
Fat
Protein
Lactose
Solids
SNF
MUN
FDP
Citric Acid
Casein pH
FFA
Fatty Acids Profile
Ketone Bodies

13. Blood BHB
Monitoring of the cows metabolic activity and detection of ketosis by infrared spectroscopy
Why predict Blood BHB instead of Milk BHB?
Milk BHB is unstable and levels very low
Milk BHB content will decrease rapidly if the milk is not stored at 4°C right after milking
The development of a calibration based on the Blood BHB allows not only
to take into account the variation of the ketone bodies but also all the
variations in the milk composition that are directly or indirectly linked to
the metabolic disorder, and particularly the milk fatty acids profile

14. Blood BHB
Calibration on 696 cow samples

15. Blood BHB
Correlation spectrum between Blood BHB content & milk spectra
The spectral zone with the highest correlation are related to the milk fatty acids profile

16. Lactoferrin
Used for early detection of mastitis & monitoring of the sanitary status of the mammary gland
Iron binding antimicrobial glycoprotein
Plays a role in pre-immune host defense
Strong association between LF & occurrence of mastitis
Used to check sanitary status of the mammary glands in the herd

17. Lactoferrin LFir (µg/mL) 77,13 0,21 0,22 0,65 0,64 0,68 LFir (µg/mL)
LFir
(µg/mL)
LFref
Log LFir
Log LFref (µg/mL)
IBC/µL
Log IBC/µL
SCC/µL
Log SCC/µL
Healthy
144,04
120,85
1,86
1,99
314
2,34 95
1,88
Accute
210,32
234,46
2,09
2,33
5592
3,53
1967
3,19
Chronic
226,98
250,66
2,15
2,38
8273
3,74
2863
3,31
Min 41 15
1,13
1,17 63
1,80 11
1,04
Max
413
412
2,45
2,62
22082
4,34
9999
4,00
Mean
194
202
2,04
2,23
4726
3,20
1642
2,79 Sd
66,02
99,03
0,24
0,28
6213,44
0,74
2242,39
0,72
Correlation between Log LFir and Log SCC with Log LFref significantly better than normal scale
Correlation between Log (Lfir) and Log(LFref) equivalent to correlation between Log SCC and Log LFref
LF seems to be a good indicator of Cow Mastitis Status and could especially be used to differentiate
healthy from accute or chronical mastitis
It has been suggested that bovine milk LF play an important role in host defense against Escherichia
Coli mastitis, On the other end, LF thought to have little antibacterial effect on Staphylococcus aureus
or Streptococcus agalactia
LFir
(µg/mL)
LFRef (µg/mL)
logLFir
LogLFRef
IBC/µL
Log IBC/µL
SCC/µL
Log SCC/µL
Sy,x/LFref
77,13
0,21
0,26
0,22
0,25
r/Log LFRef
0,65
1,00
0,42
0,64
0,43
0,68

18. Lactoferrin