1. Sugarcane Aphid, Melanaphis sacchari, and Grain Sorghum: Implications for Honeybees, Apis Mellifera
Whitney D. Crow, Angus Catchot, Jeff Gore,
Scott D. Stewart, and Sandy Steckel

2. Objectives
Determine the frequency of foraging honeybees in grain sorghum and potential implications from use of insecticide seed treatments
Impact on growth and development of cotton

3. Methods and Materials Bee Scouting Data analysis
Eight fields for three weeks
Apiary Present
Various stages of grain sorghum
Mid-day: 10:30 AM to 1:30 PM
Four observers
50 meters of row
Recorded honeybees and honeydew present
Data analysis
PROC REG and PROC MIXED SAS 9.4 for Windows
Tilled- April 9th; Telone-May 5th; Planted- May 12th;

4. Methods and Materials Pollen Analysis:
Study conducted
Starkville, MS
Jackson, TN
Randomized Complete Block Design
Four replications
Data analysis
PROC FREQ SAS 9.4 for Windows

5. Methods and Materials Pollen Analysis: Pollen Collection
50 heads
50% bloom
National Science Laboratory
Gastonia, NC
30%
60%
100%

6. Sugarcane Aphid Control: Seed Treatment/In-Furrow Insecticides
AVG # SCA/LOWER LEAF
~50 DAP

7. Honeybees: Bloom vs. Non-Bloom Grain Sorghum
P<0.001

8. Honeydew and Foraging Honeybees
P<0.001
435:1

9. Insecticide Detections
Thiamethoxam (Cruiser)
No Detection
Imidacloprid (Gaucho)
Clothianidin (Poncho)
(1) Detection at 71.6 ppb
Untreated Control
(1) Detection of clothiandin at 25.7 ppb
(1) Detection of flupyradifurone at 3.9 ppb

10. Flupyradifurone Concentrations: In-Furrow at 58 g ai ha-1
Concentration in PPB

11. Flupyradifurone Concentrations: In-Furrow at 102 g ai ha-1
(23)
Concentration in PPB

12. Pollen Consumption Estimated maximum daily intake of pollen
NOEL (no observed effect level)
For flupyradifurone at 0.79 ai µg bee-1
Estimated maximum daily intake of pollen
9.6 mg day-1
(8.9 µg bee-1)( kg pollen consumed) = µg ai bee-1
Kg pollen consumed = 88,764 mg pollen ̴ 3.13 oz

13. Conclusions Bee frequency Seed Treatments Bloom Honeydew factor
Important management tool
Detection

14. Questions