1. The Value of Optical Coherence Tomography in Glioma Surgery
David Jaehyun Park, MD, PhD
Department of Neurosurgery, Seoul St. Mary's Hospital, The Catholic University of Korea,
222 Banpo-daero, Seocho-gu, Seoul, , Korea.
Tel: , Fax: ,
David Jaehyun Park, MD, PhD¹, Jae Sung Park, MD¹, Ji Sun Woo, PhD2, Seong Muk Kim, PhD2, Yong Joon Joon, PhD3, Tae Joong Eom,PhD3, Sin Soo Jeun, MD, PhD¹
¹Department of Neurosurgery, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Korea
2Department of Biomedical Science, College of Medicine, The Catholic University of Korea, Seoul, Korea
3Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju, Korea
INTRODUCTION
A number of different methods to localize a glioma intraoperatively have been introduced. Neuronavigation, intraoperative MRI, Intraoperative sonography, and 5-aminolevulinic acid are those methods. As brain shifts after opening dura, not only localization but also detection of residual tumor have an important role in glioma surgery.
AIM
In the present study, we focused on optical coherence tomography (OCT). OCT is a non-invasive and non-contact imaging technology. It allows discrimination of normal brain, solid tumor tissue, and diffusely invaded brain tumor tissue. OCT imaging allowed acquisition of 2D and 3D data arrays for multi-planar analysis of the tumor to brain interface.
SUMMARY / CONCLUSION
RESULTS
This feasibility study has demonstrated that intraoperative use of OCT imaging can discriminate clearly between remnant tumor and normal brain. This technology could be a new innovative tool for intraoperative imaging in glioma surgery
Margins of the gliomas in xenograft mouse models were compared with both fluorescence images and OCT images. Comparing both images, OCT imaging for glioma was highly accurate. The margin of glioma was confirmed by H&E staining.
REFERENCES
MATERIAL & METHODS
In this study, we made U87-Luc human glioma cell xenograft mouse models to check the intraoperative usefulness of OCT imaging in glioma surgery. To extend OCT imaging depth, we developed an OCT system with nm wavelength range. The OCT system enhanced image contrast difference between gliomas and normal brain tissue. Fluorescence imaging and OCT imaging were done and accuracy of the images was analyzed. Moreover, hematoxylin and eosin (H&E) staining was done for each xenograft mouse model to confirm the margin of glioma. Both ex-vivo and in-vivo experiments were performed.
1.Kut C, Chaichana KL, Xi J, et al. Detection of human brain cancer infiltration ex vivo and in vivo using quantitative optical coherence tomography. Science translational medicine. Jun ;7(292):292ra100.
2.Boppart SA. Optical coherence tomography: technology and applications for neuroimaging. Psychophysiology. Jul 2003;40(4):
3.Bohringer HJ, Boller D, Leppert J, et al. Time-domain and spectral-domain optical coherence tomography in the analysis of brain tumor tissue. Lasers in surgery and medicine. Jul 2006;38(6):
4.Bohringer HJ, Lankenau E, Rohde V, Huttmann G, Giese A. Optical coherence tomography for experimental neuroendoscopy. Minimally invasive neurosurgery : MIN. Oct 2006;49(5):
5.Bohringer HJ, Lankenau E, Stellmacher F, Reusche E, Huttmann G, Giese A. Imaging of human brain tumor tissue by near-infrared laser coherence tomography. Acta neurochirurgica. May 2009;151(5): ; discussion 517.
6.Eyupoglu IY, Hore N, Savaskan NE, et al. Improving the extent of malignant glioma resection by dual intraoperative visualization approach. PloS one. 2012;7(9):e44885.
7.Assayag O, Grieve K, Devaux B, et al. Imaging of non-tumorous and tumorous human brain tissues with full-field optical coherence tomography. NeuroImage. Clinical. 2013;2:
8.Parrozzani R, Clementi M, Kotsafti O, et al. Optical coherence tomography in the diagnosis of optic pathway gliomas. Investigative ophthalmology & visual science. Dec 2013;54(13):
9.Avery RA, Hwang EI, Ishikawa H, et al. Handheld optical coherence tomography during sedation in young children with optic pathway gliomas. JAMA ophthalmology. Mar 2014;132(3):