1. Department of Medical and Clinical Biophysics Faculty of Medicine Pavol Jozef Šafárik University in Košice, Slovakia
Expertise in:
Mass spectrometry based proteomic analysis – top down and bottom-up approach, label free quantification and statistical analysis in proteomics, multidimensional protein/peptide separation including 2DnanoHPLC and 2D electrophoresis, post translational modification analysis with ETD fragmentation.
Mass spectrometers: MALDI TOF (Ultraflextreme Bruker), ion trap (Amazon speed ETD Bruker), QTOF (Agilent 6538), QQQ (Agilent 6460) – all coupled with nanoHPLC.
Proteome profiling of body fluids – blood (specifically platelets and leukocytes), urine, saliva and cerebrospinal fluid.
Proteome profiling of cell lines - breast cancer cell line MCF7 and stem cells.
Cell cultures characterization - monitoring off cell proliferation, morphology change, attachment quality in a label-free and real-time manner
Isolation of stem cells from chorion and bone marrow and their purification by magnetic nanoparticles.

2. Expected benefits and activities during participation in BIONECA:
We will share our experiences with groups working in the field of proteomics and LC-MS based analysis (e.g. method development, proteomic raw data analysis by different softwares, etc)
We will share our experiences and protocols in stem cells isolations from chorion membrane and bone marrow, purification/separation of isolated stem cells by magnetic nanoparticles.
Would like to learn from groups who are experienced in bioinformatics,especially signal pathways analysis by different open source softwares.
Would like to learn about surface/bulk modification of biodegradabile nanomaterials and their homogeneity analysis.
Would like to learn about targeted treatment in cancer therapy by using nanoparticles