1. Culturing Bacteria Growth Media
Agars… Solid (derived from marine algae)
Petri Dish … isolate colonies
Slants… to culture (grow) colonies…aerobes
Deep tubes… to culture (grow) colonies…
Checks for gas requirements
Anaerobes…deep
Aerobes surface
Facultative…mixed
Checks for motility

2. Culturing Bacteria Broth… LB (Luria Bertani) most common
Culture to grow large amounts of colonies
Amount of growth recorded by turbidity
Does not offer differential identification

3. Differential Growth Media
Differential Growth media…helps identify specific colonies
Chromogenic growth media uses color for identification
Antibiotic selection uses antibiotic rich media to select for transformed bacteria given resistance
Ampicillin most common

4. Kirby-Bauer Disk diffusion test
Test is used to determine the sensitivity of different bacteria to antibiotics
Zone of inhibition (no growth) describes either the resistance by the bacteria to antibiotics or the sensitivity of the bacteria to the antibiotics

5. Streak Plating Bacteria
Bacterial study requires isolation of a single colony of bacteria
Since most objects contain many different species of bacteria a streak plate gives the observer an opportunity to choose a single colony for study
Serial streak plates are even more effective

6. Serial Dilution Method to Reduce Bacteria
Often the bacteria are to numerous to effectively separate on a single streak plate.
A serial dilution decreases the bacteria count 10X with each dilution
Each dilution is plated to get an idea of the amount of bacteria present by counting the CFUs (colony forming units)
#of colonies raised x 10 to the power of the # of dilutions
Unit is in CFU/ml
(21CFU x 105) x 1000ml
100ml ml