1. glomerular homogenate
Supplementary Figure １ A C
Rat
glomerular homogenate
Conditions and specific primers for real-time PCR methods
Target mRNA
Human GLP-1 receptor
Rat GLP-1 receptor
Preincubation
95ºC, 30 s
PCR
92ºC, 1 s
62ºC, 10 s
72ºC, 15 s
Sense primer
5’-GTT CCC CTG CTG TTT GTT GT-3’
5'-ATC CAC CTG AAC CTG TTT GC-3'
Antisense primer
5’-CTT GGC AAG TCT GCA TTT GA-3’
5'-GCA GTA TTG CAT GAG CAG GA-3'
Product size
228 bp
174 bp
200
100
Rat GLP-1R
174bp 50 B
NHMCs
300
200
Human GLP-1R
228bp
100 50
Supplementary Fig. 1 The expression of GLP-1 receptor mRNA in rat glomerular homogenates (a) and normal human mesangial cells (NHMCs) (b) used in the experiments were confirmed by real-time PCR analysis. Conditions and specifics are indicated in C.