1. Products > NCI-H1299 Transfection Reagent (Lung Adenocarcinoma)
Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H1299 Transfection Reagent (Lung Adenocarcinoma)
Altogen Biosystems offers the NCI-H1299 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
NCI-H1299 Transfection Reagent is an advanced formulation of lipid based reagent, and it has been developed to provide high transfection efficiency with NCI-H1299 and other lung cancer cell lines.
This cell line is a good host for studying lung cancer and related areas of study. When cultured in vitro, NCI-H1299 cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase NCI-H1299 Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. High Density NCI-H1299 Cells atcc.org
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H1299 Transfection Reagent (Lung Adenocarcinoma)
NCI-H1299 Cell Line Description and Applications
The NCI-H1299 line originates from the lung cells of a 43-year old Caucasian male patient suffering from non-small cell lung cancer. The patient received radiation prior to cell line establishment. The cell line is specifically derived from a lymph node metastatic site. The NCI-H1299 cell line displays epithelial morphology and stain positive for keratin and vimentin and produce neuromedin B. The NCI-H1299 cell line has a partial deletion of the p53 protein and lacks expression of the p53 protein. This cell line is particularly useful research related to lung cancer.
High Density NCI-H1299 Cells atcc.org
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. NCI-H1299 Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H1299 Transfection Reagent (Lung Adenocarcinoma)
NCI-H1299 Transfection Protocol
1. Plate 10, ,000 NCI-H1299 cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with NCI-H1299 cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of NCI-H1299 cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H1299 Transfection Reagent (Lung Adenocarcinoma)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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5. NCI-H1299 Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H1299 Transfection Reagent (Lung Adenocarcinoma)
NCI-H1299 Transfection Kit Product Details
Two component formulation enhances lipid mediated transfection efficiency
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
High transfection efficacy in the presence of serum
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
Reproducible transfection results
Works well for standard reverse transfection and high-throughput applications
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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6. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H1299 Transfection Reagent (Lung Adenocarcinoma)
Data
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the NCI-H1299 cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPD mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=5).

7. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H1299 Transfection Reagent (Lung Adenocarcinoma)
Data
Figure 2. Protein expression of GAPDH in NCI-H1299 cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into NCI-H1299 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. NCI-H1299 Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > NCI-H1299 Transfection Reagent (Lung Adenocarcinoma)
NCI-H1299 Transfection Kit Benefits
Pre-optimized transfection protocol for NCI-H1299 cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use NCI-H1299 transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable NCI-H1299 cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   