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4 A Tour of the Cell.

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1 4 A Tour of the Cell

2 Which cellular structure is common to all three domains of life?
nucleus endoplasmic reticulum mitochondria ribosomes endocytotic vesicles Answer: D. Ribosomes, though not the same size in eukaryotes and prokaryotes, are essential for protein synthesis, which is required for life. Discuss examples of essential proteins. Please see Concept 4.2. © 2016 Pearson Education, Inc.

3 Which cellular structure is common to all three domains of life?
nucleus endoplasmic reticulum mitochondria ribosomes endocytotic vesicles © 2016 Pearson Education, Inc. 3

4 Enzymes responsible for biosynthesis of membrane lipids would be located in what part of the cell?
endoplasmic reticulum nucleus lysosomes Golgi plasma membrane Answer: A . This question focuses on Concepts 4.2 and 4.4. The endoplasmic reticulum produces membrane lipids and sends them throughout the cell. © 2016 Pearson Education, Inc. 4

5 Enzymes responsible for biosynthesis of membrane lipids would be located in what part of the cell?
endoplasmic reticulum nucleus lysosomes Golgi plasma membrane © 2016 Pearson Education, Inc. 5

6 Which of the following is not an argument for the theory that mitochondria and chloroplasts evolved from prokaryotic endosymbionts? Mitochondria and chloroplasts have double membranes. Mitochondria and chloroplasts have their own ribosomes. Mitochondria and chloroplasts have their own DNA. The mitochondrial and chloroplast genomes are circular. All of the above support the endosymbiotic theory. Answer: E. This question focuses on Concept 4.5. Discuss from what groups of prokaryotes the mitochondria and chloroplasts are thought to have come. © 2016 Pearson Education, Inc. 6

7 Which of the following is not an argument for the theory that mitochondria and chloroplasts evolved from prokaryotic endosymbionts? Mitochondria and chloroplasts have double membranes. Mitochondria and chloroplasts have their own ribosomes. Mitochondria and chloroplasts have their own DNA. The mitochondrial and chloroplast genomes are circular. All of the above support the endosymbiotic theory. © 2016 Pearson Education, Inc. 7

8 Autophagy removes old, damaged organelles like mitochondria
Autophagy removes old, damaged organelles like mitochondria. Which organelle is most critical for autophagy? smooth ER peroxisomes rough ER lysosomes Golgi apparatus Answer: D. Lysosomes perform the digestion that is part of autophagy, as described in Concept 4.4. © 2016 Pearson Education, Inc. 8

9 Autophagy removes old, damaged organelles like mitochondria
Autophagy removes old, damaged organelles like mitochondria. Which organelle is most critical for autophagy? smooth ER peroxisomes rough ER lysosomes Golgi apparatus © 2016 Pearson Education, Inc. 9

10 What protein(s) is (are) not made at the rough ER?
insulin digestive enzymes of the gut antibodies in the blood enzymes found within the peroxisome organelle collagen (an extracellular matrix protein) Answer: D. Concept 4.4 discusses how the rough ER produces proteins that are sent outside the cell by exocytosis. © 2016 Pearson Education, Inc. 10

11 What protein(s) is (are) not made at the rough ER?
insulin digestive enzymes of the gut antibodies in the blood enzymes found within the peroxisome organelle collagen (an extracellular matrix protein) © 2016 Pearson Education, Inc. 11

12 What is the correct order for secretion?
rough ER, endosome, Golgi, smooth ER rough ER, Golgi, smooth ER, exocytosis smooth ER, rough ER, exocytosis, Golgi rough ER, Golgi, transport vesicle, exocytosis rough ER, Golgi, endosome, exocytosis, transport vesicle Answer: D. Secreted proteins, as shown in Concept 4.4, pass through several parts of the endomembrane system. © 2016 Pearson Education, Inc. 12

13 What is the correct order for secretion?
rough ER, endosome, Golgi, smooth ER rough ER, Golgi, smooth ER, exocytosis smooth ER, rough ER, exocytosis, Golgi rough ER, Golgi, transport vesicle, exocytosis rough ER, Golgi, endosome, exocytosis, transport vesicle © 2016 Pearson Education, Inc. 13

14 Brefeldin A disrupts transport from the ER to the Golgi apparatus
Brefeldin A disrupts transport from the ER to the Golgi apparatus. What other structures would be affected by Brefeldin A? lysosomes, transport vesicles, plasma membrane mitochondria, peroxisomes, plasma membrane vacuoles, mitochondria, plasma membrane lysosomes, transport vesicles, nuclear membrane all intracellular organelles and membranes Answer: A . This question corresponds with Concept 4.4. The connection being made is with the various parts of the endomembrane system. © 2016 Pearson Education, Inc. 14

15 Brefeldin A disrupts transport from the ER to the Golgi apparatus
Brefeldin A disrupts transport from the ER to the Golgi apparatus. What other structures would be affected by Brefeldin A? lysosomes, transport vesicles, plasma membrane mitochondria, peroxisomes, plasma membrane vacuoles, mitochondria, plasma membrane lysosomes, transport vesicles, nuclear membrane all intracellular organelles and membranes © 2016 Pearson Education, Inc. 15

16 Since alcohol is destroyed in the cell by the removal of hydrogen atoms, where does this occur?
lysosome smooth ER peroxisome rough ER Golgi apparatus Answer: C. The text discusses the reactions that destroy peroxides, and students should see a parallel in alcohol and its destruction. Please see Concept 4.5. © 2016 Pearson Education, Inc. 16

17 Since alcohol is destroyed in the cell by the removal of hydrogen atoms, where does this occur?
lysosome smooth ER peroxisome rough ER Golgi apparatus © 2016 Pearson Education, Inc. 17

18 Taxol, which is used in fighting breast cancer, prevents depolymerization of microtubules. What cellular function might taxol interfere with most in fighting cancer? maintaining cell shape cell motility (cilia or flagella) chromosome movements in cell division cell division (cleavage furrow formation) cytoplasmic streaming Answer: C. Please see Concept 4.6. Microtubules are critical for the cell divisions that allow cancer to grow and spread. Discuss how microtubules act during cell division. © 2016 Pearson Education, Inc. 18

19 Taxol, which is used in fighting breast cancer, prevents depolymerization of microtubules. What cellular function might taxol interfere with most in fighting cancer? maintaining cell shape cell motility (cilia or flagella) chromosome movements in cell division cell division (cleavage furrow formation) cytoplasmic streaming © 2016 Pearson Education, Inc. 19

20 What role do integrins play in cells?
speed cell division link extracellular matrix and cell interior maintain numbers of mitochondria act as ion channels connect parts of the endomembrane system Answer: B. Integrins are integral membrane proteins that provide a connection across the plasma membrane. Discuss examples where this is critical for life. © 2016 Pearson Education, Inc.

21 What role do integrins play in cells?
speed cell division link extracellular matrix and cell interior maintain numbers of mitochondria act as ion channels connect parts of the endomembrane system © 2016 Pearson Education, Inc.

22 Which kind of cells make plasmodesmata?
plant cells animal cells bacterial cells A–C B and C Answer: A. Plasmodesmata are hollow channels through the plant cell wall. Please see Concept 4.7. © 2016 Pearson Education, Inc.

23 Which kind of cells make plasmodesmata?
plant cells animal cells bacterial cells A–C B and C © 2016 Pearson Education, Inc.

24 Which kind of cells make tight junctions?
plant cells animal cells bacterial cells A–C A and C Answer: B. Tight junctions help to prevent movement of material from one side of an animal cell to the other via the extracellular matrix. Discuss how this is important in the small intestine. Please see Concept 4.7. © 2016 Pearson Education, Inc.

25 Which kind of cells make tight junctions?
plant cells animal cells bacterial cells A–C A and C © 2016 Pearson Education, Inc.

26 If you were studying frog eggs, what would be most useful for visualizing them?
unaided eye light microscope electron microscope A and B A and C Answer: D. Frog eggs are fairly large cells and so can easily be viewed using both the unaided human eye and the light microscope. Please see Concept 4.1. © 2016 Pearson Education, Inc.

27 If you were studying frog eggs, what would be most useful for visualizing them?
unaided eye light microscope electron microscope A and B A and C © 2016 Pearson Education, Inc.

28 If you were studying typical bacteria, what would be most useful for visualizing them?
unaided eye light microscope electron microscope A and B B and C Answer: E. Most bacteria are too small to detect with the unaided eye, but some detail can be seen with the light microscope and much more using the electron microscope. Please see Concept 4.1. © 2016 Pearson Education, Inc.

29 If you were studying typical bacteria, what would be most useful for visualizing them?
unaided eye light microscope electron microscope A and B B and C © 2016 Pearson Education, Inc.

30 If you were asked to use a light microscope to locate specific molecules in a cell, what would be the best form of light microscopy to use? Nomarski phase-contrast brightfield, unstained brightfield, stained fluorescence Answer: E. Fluorescence microscopy gives highly specific data on the location of molecules. Discuss how it could be used to locate nuclei in cells. Please see Concept 4.1. © 2016 Pearson Education, Inc.

31 If you were asked to use a light microscope to locate specific molecules in a cell, what would be the best form of light microscopy to use? Nomarski phase-contrast brightfield, unstained brightfield, stained fluorescence © 2016 Pearson Education, Inc.

32 Which is not part of a prokaryotic cell?
nucleoid chloroplast ribosome plasma membrane cell wall Answer: B. Chloroplasts are descended from prokaryotic cells, but they are found on eukaryotes. Please see Concept 4.2. © 2016 Pearson Education, Inc.

33 Which is not part of a prokaryotic cell?
nucleoid chloroplast ribosome plasma membrane cell wall © 2016 Pearson Education, Inc.

34 Which is not part of a eukaryotic cell?
nucleoid chloroplast ribosome plasma membrane peroxisome Answer: A. The eukaryotes keep most of their DNA in a membrane-enclosed nucleus, not a nucleoid. Please see Concept 4.2. © 2016 Pearson Education, Inc.

35 Which is not part of a eukaryotic cell?
nucleoid chloroplast ribosome plasma membrane peroxisome © 2016 Pearson Education, Inc.

36 If you fluorescently labeled a protein and found it ended up in the nucleus, through what must it have passed? pore complex nucleolus chromatin endoplasmic reticulum not necessarily any of these Answer: A. Pore complexes are the gateways into the nucleus. Please see Concept 4.3. © 2016 Pearson Education, Inc.

37 If you fluorescently labeled a protein and found it ended up in the nucleus, through what must it have passed? pore complex nucleolus chromatin endoplasmic reticulum not necessarily any of these © 2016 Pearson Education, Inc.

38 Where would you place an arrow on a diagram of a cell if the arrow was to point to the place where cytoplasmic proteins are made? rough ER smooth ER free ribosomes peroxisome mitochondrion Answer: C. Soluble proteins found in the cytoplasm are made by ribosomes, which float freely in the cytoplasm, not attached to any organelle. Please see Concept 4.3. © 2016 Pearson Education, Inc.

39 Where would you place an arrow on a diagram of a cell if the arrow was to point to the place where cytoplasmic proteins are made? rough ER smooth ER free ribosomes peroxisome mitochondrion © 2016 Pearson Education, Inc.

40 If you placed fluorescently labeled protein outside an animal cell, after uptake for digestion, where would it be? rough ER smooth ER lysosome peroxisome mitochondrion Answer: C. Lysosomes digest at the cellular level. Discuss what lysosomal diseases might cause in a human being. Please see Concept 4.4. © 2016 Pearson Education, Inc.

41 If you placed fluorescently labeled protein outside an animal cell, after uptake for digestion, where would it be? rough ER smooth ER lysosome peroxisome mitochondrion © 2016 Pearson Education, Inc.

42 If you were examining a plant cell, what kind of vacuole would you find?
contractile central food A–C A and C Answer: B. Plant cells have a proportionally large central vacuole that can perform a number of different functions, depending on the state of the plant cell. Please see Concept 4.4. © 2016 Pearson Education, Inc.

43 If you were examining a plant cell, what kind of vacuole would you find?
contractile central food A–C A and C © 2016 Pearson Education, Inc.

44 If you were working in a cave and discovered an unusual carnivorous plant, what might this plant lack at the cellular level yet still be able to survive using carnivory? ER nucleus mitochondrion chloroplast peroxisome Answer: D. Chloroplasts would be useless in a lightless cave, though the other cellular parts would be needed for daily life. Discuss what parts of a plant cell are most critical for normal plant carnivory. Please see Concept 4.5. © 2016 Pearson Education, Inc.

45 If you were working in a cave and discovered an unusual carnivorous plant, what might this plant lack at the cellular level yet still be able to survive using carnivory? ER nucleus mitochondrion chloroplast peroxisome © 2016 Pearson Education, Inc.

46 If you applied a drug to block mitochondrial function, what would an affected cell lack most?
protein ATP enzymes vesicles membranes Answer: B. The mitochondrion is the powerhouse of the cell and provides vitally important supplies of ATP. Please see Concept 4.5. © 2016 Pearson Education, Inc.

47 If you applied a drug to block mitochondrial function, what would an affected cell lack most?
protein ATP enzymes vesicles membranes © 2016 Pearson Education, Inc.

48 A drug that blocks muscular contraction might have disrupted what?
nucleus intermediate filaments microtubules ribosomes microfilaments Answer: E. Actin microfilaments work with myosin to produce muscular contraction. Please see Concept 4.6. © 2016 Pearson Education, Inc.

49 A drug that blocks muscular contraction might have disrupted what?
nucleus intermediate filaments microtubules ribosomes microfilaments © 2016 Pearson Education, Inc.

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