1. ABX Pentra DF 120 Flexible hematology platform For internal use only
Prepared by L.Castellane

2. Main features analysis Methods and technologies Pentra ML
ABX Pentra DF 120
Main features analysis
Methods and technologies
Pentra ML
Prepared by L.Castellane

3. Optional slide maker and stainer
Main features
Rack loader
Reagent compartment
Technical
software
Processed tubes
Stat Sample Bay
ABX Pentra ML
Optional slide maker and stainer
Prepared by L.Castellane

4. IMmature Lymphocyte, Monocyte and Granulocyte
Main features
CBC, DIFF
IMmature Lymphocyte, Monocyte and Granulocyte
Optional and integrated slide maker and stainer
Expert Validation Station
Prepared by L.Castellane

5. Wbc, Rbc, Hgb, Hct, Mcv, Mch, Mchc, Rdw, Plt, Mpv, Pct, Pdw
Main features
32 Parameters
CBC (12 parameters)
Wbc, Rbc, Hgb, Hct, Mcv, Mch, Mchc, Rdw, Plt, Mpv, Pct, Pdw
Differential count (12+20 parameters)
Lym, Mon, Neu, Eos, Baso, Aly, Lic (% and #)
IML, IMM, IMG (% and #)
Throughput (12O samples per hour)
Prepared by L.Castellane

6. 5 reagents Unique blood control ABX Difftrol - 3 levels ABX Diluent
Main features
5 reagents
ABX Diluent
ABX Basolyse
ABX Leucodiff
ABX Alphalyse or ABX Lysebio (Cyanide free)
ABX Cleaner
Unique blood control
ABX Difftrol - 3 levels
Target values on disk, multi-language package insert
ABX reagents and controls are bar-coded and CE marked
Batch number, level detection, small reagent volumes, waste level sensor
Prepared by L.Castellane

7. External and internal bar-code reader
Main features
3 types of analyses (with random access)
CBC
DIFF
SPS
External and internal bar-code reader
Prepared by L.Castellane

8. Main features analysis Methods and technologies Pentra ML
ABX Pentra DF 120
Main features analysis
Methods and technologies
Pentra ML
CBC principles
5 Diff – Double matrix
Prepared by L.Castellane

9. Methods & technologies
RBC/PLT - Impedance method
HGB - Cyanmethemoglobin or cyanide free
WBC - (Triangulation)
-> WBC channel (impedance)
-> BASO channel (Specific lyse + impedance)
-> 5Diff channel (optical)
Prepared by L.Castellane

10. Methods & technologies
5Diff Principles 
1. Cytochemistry
Lysing of red blood cells
Staining of nuclei and cytoplasmic granules of leukocytes
Fixing the leukocytes in their original state (48 hours post-draw stability)
2. Double Hydrodynamic Sequential System (DHSS)
Flow cytometer with hydrodynamic focusing before and after the micro-aperture
3. Impedance method
Measurement of the cell volume (“X” axis)   
4. Light absorbance
Internal structure (“Y” axis)
Prepared by L.Castellane

11. Methods & technologies
Double Diff Matrix
Eosinophils
Large Immature Cells
Neutrophils
IMmature Granulocytic line
Lymphocytes
IMmature Monocytic line
Monocytes
IMmature Lymphocytic line
Atypical LYmphocytes
Prepared by L.Castellane

12. Methods & technologies
IMmature Granulocytes
Myeloblast
IMG
Promyelocyte
Myelocyte
Metamyelocyte
IMG % and # Identification of Granulocytic cell line
count of the overall myelemia
Prepared by L.Castellane

13. Methods & technologies
IMmature Monocyte
Monoblast
Promonocyte
IMM
IMM % and # Identification of Monocytic cell line
Count of the precursors of the monocytic line
Prepared by L.Castellane

14. Methods & technologies
IMmature Lymphocyte
Lymphoblast
Prolymphocyte
IML
IML % and # Identification of Lymphocytic cell line
IML and ALY include all abnormal lymphocytic populations
Prepared by L.Castellane

15. Methods & technologies
Routine identification and quantification of 3 immature cell lines involved in leucopoiesis:
Granulocytic line
Monocytic line
Lymphocytic line
-> Reduction of the number of slide examinations
-> Diagnostic and follow-up tool for rapid decision-making
Prepared by L.Castellane

16. Methods & technologies
1- Priority alarms
2- Separation alarms
ALY: (Atypical Lymphocytes)
Atypical Lymphocytes
Plasmocytes, hairy Cells...
Ne: (Neutro-eosino)
Hyper-segmented neutrophils
Hypo-granular eosinophils...
Mn: (Mono-neutro)
Immature granulocytes
Band cells...
LIC: (Large Immature Cells)
Blasts, myelocytes
Promyelocytes
Metamyelocytes...
Nl: (Neutro-lympho)
Hypo-segmented neutrophils
Sezary cells, hairy cells...
Prepared by L.Castellane

17. Methods & technologies
3- Zone alarms
No: (Noise)
Platelet aggregates, NRBCs
Lysis resistant RBCs
Ln: (Left-neutro)
Cellular debris
Ll, Ll1: (Left-lympho)
Platelet aggregates, NRBCs
Rn: (Right-neutro)
Immature granulocytes
Rm: (Right-mono)
Large monocytes, blasts
Prepared by L.Castellane

18. Methods & technologies
CHRONIC MYELOID LEUKEMIA
Pentra DF 120 result:
NEU %
EOS %
LYM %
MON %
BAS %
IMG %
IMM %
IML %
ALY %
Prepared by L.Castellane

19. Methods & technologies
ACUTE MONOBLASTIC & MONOCYTIC LEUKEMIA
Pentra DF 120 result:
NEU %
EOS %
LYM %
MON %
BAS %
IMG %
IMM %
IML %
ALY %
Prepared by L.Castellane

20. Methods & technologies
T-CELL PROLYMPHOCYTIC LEUKEMIA
Pentra DF 120 result:
NEU %
EOS %
LYM %
MON %
BAS %
IMG %
IMM %
IML %
ALY %
Prepared by L.Castellane

21. Main features analysis Methods and technologies Pentra ML
ABX Pentra DF 120
Main features analysis
Methods and technologies
Pentra ML
Specs & functionality
Prepared by L.Castellane

22. 17 inch flat colour screen Windows XP CD-ROM burner Automatic rerun
Specs & functionality
Dedicated PC
17 inch flat colour screen
Windows XP
CD-ROM burner
Automatic rerun
Reflex testing
Pre-diluted mode
Cytology library
Onboard user manual
Prepared by L.Castellane

23. Mono or bi-directional connection:
Specs & functionality
Mono or bi-directional connection:
ASTM format
Transfer of
Demographic data (blood type - man, woman, child)
Tests requested (CBC, CBC/DIFF, SPS)
Up to 3 ABX Hematology analyzers (Pentra range) can be connected
Query mode
Download capability
Prepared by L.Castellane

24. User defined rules applied when : receiving the worklist,
Specs & functionality
User defined rules applied when :
receiving the worklist,
a sample has been analyzed.
_ Rerun capability
_ Reflex testing
_ Automatic validation
_ Messages, comments….
Bio-technical validation with :
Patient history
Delta check
Result edition
Manual entry
Recalculation capability
Prepared by L.Castellane

25. Bio-technical validation with :
Result screens
Bio-technical validation with :
Patient report, run, re-run, patient history, graphs, alarms. All displayed on the same screen.
Delta check
Result edition
Manual entry
Recalculation capability
1- Title bar
2- Patient and Graphics area
3- Messages
4- Patient Comments
5- Technical Comments
6- Extended Diff
7- Result area
8- Report
9- Runs
Prepared by L.Castellane

26. Hematovision IV included A cell cytology library
Specs & functionality
Hematovision IV included
A cell cytology library
Prepared by L.Castellane

27. ABX Pentra 120 series
Prepared by L.Castellane

28. ABX Pentra 120 series
Prepared by L.Castellane

29. Thank you
Prepared by L.Castellane