1. Biomolecular X-ray Crystallography Facility
Dave McKay
JSCBB C1B51

2. Need:
Have:
protein, RNA, …
crystals
diffraction
Want:
structure
model

3. One option for crystallization: robotic dropsetter
Need typically:
concentration: ~10 mg/ml
amount: ~ microL
=> milligrams
(typically, dropsize is 0.2 microL)

4. For imaging, microscopes or automatic imager
crystallization plate “hotel”

5. For imaging, microscopes or automatic imager

6. For imaging, microscopes or automatic imager

7. In-house data collection: rotating anode, image plate, crystal freezing system

8. Synchrotron data collection: ship frozen crystals and collect data remotely

9. Synchrotron data collection: ship frozen crystals and collect data remotely

10. For remote data collection and data analysis

11. Biomolecular X-ray Crystallography Facility
Dave McKay
JSCBB C1B51