1. Lab Management

2. Have students match the picture for set-ups

3. Set Up for each PAIR Gel box Waste basket Tip box p20 micropipet
Paper towel

4. Proper lab technique

5. Not so proper lab technique

6. Good for EL students

7. CLEAN UP

8. Clean Up
Match the picture.

9. Cartoon Transitions

10. What’s going on in there?

11. What’s going on in there? Opposites attract_ + _ + _ +

12. What’s going on in there? Smaller pieces travel faster

13. CRIME SCENE – Ms. Yeung’s desk.

14. CRIME SCENE – Ms. Yeung’s desk.
WHO CHANGED ALL THE GRADES IN MS. YEUNG’S GRADEBOOK INTO F’S??

15. EVIDENCE #1 - Foot prints#2 #3 #1

16. Suspect #1 K. Minor last seen making a sandwich at Ms. Yeung’s desk
Motive – new teacher, may have been an accident she’s unwilling to admit to

17. Suspect #2 J. Ezrati
used Ms. Yeung’s computer to look up SJ Sharks game info
Motive - jealousy, Ms. Yeung stole his favorite students; likes to stick it to “the man”

18. Suspect #3 S. Ighanian
rummaged through Ms. Yeung’s desk in search of “stickers”
Motive – Freshman class advisor, wants her class to look better

19. EVIDENCE #2 – Strand of hair

20. How do you identify a suspect?
Restriction Enzymes – enzymes that cut up DNA segments at a particular sequence of bases
ie. EcoR1 (originated from E. coli)
G A A T T C
C T T A A G

21. How do you identify a suspect?
Each suspect’s DNA will be cut into a combination of long and short strands that will be unique to that suspect
T T G A A T T C G G C G G A A T T C A T A G G C T G G G C C
A A C T T A A G C C G C C T T A A G T A T C C G A C C C G G
T T G G C C G A A T T C G G C G G C A T T C A T A G G C T G A A C C C G G T T A A G C C G C C G T A A G T A T C C G A C

22. How do you identify a suspect?
We’ll use gel electrophoresis to separate out the different sized DNA segments
Each suspect will have a unique pattern on the gel that we will compare to the DNA from the crime scene (CS)

23. How do you identify a suspect?
U = Uncut (did not add restriction enzyme)
How many bands should we expect to see on our gel for the “U” tube?
Only 1 because the DNA strand did not get cut
Should the band travel close to the well or far from the well?
Close, because it’s one long strand

24. S3 S2 S1 CS U