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Figure 1 Chemical structures of MAG used in this study

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Presentation on theme: "Figure 1 Chemical structures of MAG used in this study"— Presentation transcript:

1 Figure 1 Chemical structures of MAG used in this study
From: Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy Acta Biochim Biophys Sin (Shanghai). 2009;41(12): doi: /abbs/gmp099 Acta Biochim Biophys Sin (Shanghai) | © The Author Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

2 Figure 2 Scavenging activities of MAG on hydroxyl radical
From: Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy Acta Biochim Biophys Sin (Shanghai). 2009;41(12): doi: /abbs/gmp099 Acta Biochim Biophys Sin (Shanghai) | © The Author Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

3 Figure 3 Scavenging activities of MAG on superoxide anion
From: Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy Acta Biochim Biophys Sin (Shanghai). 2009;41(12): doi: /abbs/gmp099 Acta Biochim Biophys Sin (Shanghai) | © The Author Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

4 Figure 4 Scavenging activities of MAG on hydrogen peroxide
From: Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy Acta Biochim Biophys Sin (Shanghai). 2009;41(12): doi: /abbs/gmp099 Acta Biochim Biophys Sin (Shanghai) | © The Author Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

5 Figure 5 Sensitization action of MAG on singlet oxygen
From: Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy Acta Biochim Biophys Sin (Shanghai). 2009;41(12): doi: /abbs/gmp099 Acta Biochim Biophys Sin (Shanghai) | © The Author Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

6 Figure 6 Inhibitory effect of MAG on lipid peroxidation
From: Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy Acta Biochim Biophys Sin (Shanghai). 2009;41(12): doi: /abbs/gmp099 Acta Biochim Biophys Sin (Shanghai) | © The Author Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

7 Figure 7 MAG inhibits K562 cells growth Equal amounts of inoculants (1
Figure 7 MAG inhibits K562 cells growth  Equal amounts of inoculants (1.0 × 10<sup>5</sup> viable cells) were seeded in the cultures containing different concentrations of MAG (0–10 µM). Cells at each time interval were stained using 0.2% Trypan blue solution, and viable cells were counted using a hemacytometer under light microscopy. Results represent the mean ± SD of three independent experiments. From: Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy Acta Biochim Biophys Sin (Shanghai). 2009;41(12): doi: /abbs/gmp099 Acta Biochim Biophys Sin (Shanghai) | © The Author Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

8 Figure 8 Effects of genistein on apoptotic  Data were assessed 24 h of incubation. Results represent mean ± SD of three independent experiments. *P < 0.05 vs control. From: Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy Acta Biochim Biophys Sin (Shanghai). 2009;41(12): doi: /abbs/gmp099 Acta Biochim Biophys Sin (Shanghai) | © The Author Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

9 Figure 9 Effects of MAG on cell survival and apoptosis 24 h after ALA-PDT irradiation  K562 cells were incubated for 20 min prior ALA-PDT irradiation with different concentrations of MAG (0–10 µM). The fraction of live cells in the control approach was set as 100%. Live cells were counted using a hemacytometer under light microscopy after stained the cells with 0.2% Trypan blue solution. Calculation of apoptotic cells was performed after staining the cells with Annexin V-FITC-PI by flow-cytometry analysis. Results represent the mean ± SD of three independent experiments. <sup>#</sup>P < 0.05; *P < 0.05 vs control; **P < 0.05 vs ALA-PDT. From: Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy Acta Biochim Biophys Sin (Shanghai). 2009;41(12): doi: /abbs/gmp099 Acta Biochim Biophys Sin (Shanghai) | © The Author Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

10 Figure 10 Effects of NaN<sub>3</sub> combined with MAG on apoptotic cells in ALA-PDT treatment  K562 cells were incubated for 20 min prior ALA-PDT irradiation with different concentrations of MAG (0–10 µM) and NaN<sub>3</sub>. Results represent the mean ± SD of three independent experiments. *P < 0.05 vs NaN<sub>3</sub>+PDT. From: Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy Acta Biochim Biophys Sin (Shanghai). 2009;41(12): doi: /abbs/gmp099 Acta Biochim Biophys Sin (Shanghai) | © The Author Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

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