1. Fluorophos test
The fluorophos test works by measuring the light emitted when a monophosphoric ester (mPE)is added to milk.
The amount of light emitted is proportionate to the amount of residual alkaline phosphatase present in the milk.

2. Fluorophos test
Residual
Alkaline phosphatase
ALP

3. Fluorophos test +
monophosphoric ester
ALP
mPE

4. Fluorophos test +
Light
ALP
mPE

5. Pasteurised milk
72°C/15 Secs
63°C/30 Mins
ALP

6. Pasteurised milk
72°C/15 Secs A
ALP P
63°C/30 Mins L +
mPE

7. Pasteurised milk
72°C/15 Secs A
ALP P
63°C/30 Mins L +
mPE
No light

8. Fluorophos test
The more light that is measured means that there is more residual alkaline phosphatase present in the milk.
A reading > 350 mU/L suggests that the heat treatment has not been successful or the milk has been contaminated.