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Published byMartina Charles Modified over 6 years ago
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Hydrogen production in algal-bacterial cultures
Dr. Gergely Ernő Lakatos
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Stress induced algal hydorgen production methodes
Stress induced algal hydrogen production methodes under illumination: Sulfur Nitrogen Phosphate Magnesium DEPRIVATION Disadvantages: Separated biomass and hydrogen production OR Precise addition of the deprived nutrient Slow hydrogen production initiation (1-8 days) Inhibited biomass growth
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Setup of algal-bacterial hydrogen producing system
Tris-Acetate-Phosphate media Carbon source: acetic acid. O2 CO2
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Obtained results Establishing pure algal and bacterial cultures from infected algal cultures Determination of the algal and bacterial strains Algae: Chlamydomonas Chlorella Scenedesmus Bacteria: Wilde types (detected in the algae culture): Rhodcoccus sp. Brevundimonas sp. Leifsonia sp.
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Obtained results Advantges:
Mixed biomass production during hydrogen evolution Elevated algal biomass production by the addition of bacterial partner Cooperation: biogas production by the produced algal-bacterial biomass Fast oxygen consumption by the bacterial partner Fast initiation of hydrogen production in 1-4 hours Disadvantages: Short hydrogen production time length Low hydrogen ptoduction rates
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Obtained results Elevation of hydrogen production rate by:
the application of hydrogenase defficient bacterial partner Artificially added strains (wild and hydrogenase defficient): Esherichia coli Ralstonia eutropha the optimization of gas-to-liquid phase ratios the optimization of culture optical density the reduction of algal cell size Optimized hydrogen production for daily harvesting (~100 ml H2 L-1 culture day-1), comperable hydrogen yield with sulfur-deprived results
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Short term aim Establishing a semi-continuous hydrogen and biomass producing system in small scale (40 ml). Hydrogen release by purging of the headspace 1 day H2 H2 ambient air H2 H2 H2 low biomass content high biomass content acetic acid addition 40 mL sealed bottles Biomass harvesting
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Long term aim 1. Finding cheap carbon source: using carbon dioxide as a sole carbon source. Hydrogen release by air purging 1 day H2 H2 ambient air H2 H2 H2 low biomass content high biomass content CO2 addition 40 mL sealed bottles Biomass harvesting
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Long term aim 2. Finding cheap carbon source: using diluted biogas sludge supernatant as a media for biomass and hydrogen production. biogas production biomass fermentation filtration biomass production hydrogen production dilution algae addition
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Thank you for your attention
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