1. Trait Testing: What is needed? Testing for non-GMO seed lots
Emily Dierking, PhD, Genetics Program Director

2. Topics Non-GMO Testing Why this type of testing is needed
DNA-based: RT-PCR
Why this type of testing is needed
How the testing is conducted

3. Why is non-GMO testing needed?
Provide a quality product
To ensure conventional seed is free of GMOs
To meet licensing agreement requirements
To export
Truth in labeling

4. How is non-GMO testing conducted?
3000 seed test
3 replicates of 1000 seeds each
0.1% (1/1000) detection limit
Controls included with every sample
Method:
Semi-Quantitative Real-Time PCR – DNA based
99% confidence, results reported as positive or negative at 0.1%, for positive results a half-percent interval can be indicated

5. How is non-GMO testing conducted?
All corn GMO events have 35S and/or NOS
All soybean GMO events have 35S, NOS, and or P4FMV
DNA

6. What do non-GMO testing results mean?
Negative: no GMO presence found
Positive: make decisions about rejecting or blending
Each company may have a unique threshold
European tolerance is 0.9%
Sample Information
Customer Information

7. www.indianacrop.org 765-523-2535 dierking@indianacrop.org

9. GMO Detection Method: DNA-based, RT PCR
Quality Standards for DNA Extraction
260:280 ratio
Quantified – 200 ng starting DNA
Quality Standards for Results
Certified reference materials
Standards included with each run on the machine
0.1, 0.5, [0.7, 0.9], 1.0, 2.5, 10