1. Supplemental Figure-1 De Camilli
Cyto+ATP+GTP
Cyto+ATP+GTPγS
Surface before incubation
Surface after incubation
% of Area Reduction

2. Supplemental Figure-2 De Camilli
TfR-Phluorin
0min
10min
Normalized intensity b
PM-GFP
0min
10min
Normalized intensity c
0min
10min
Normalized intensity
pixel

3. Supplemental Figure-3 De Camilli
-FBP17
-dynamin
dynamin
FBP17
Intensity (a.u.) μm
-dynamin
-endophilin
endophilin
dyn1
Intensity (a.u.) μm
-FBP17
-endophilin
endophilin
FBP17
Intensity (a.u.)
Z (μm)
1 μm
Away from surface Closer to surface

4. Supplemental Figure-4 De Camilli
Cyto + ATP + GTPγS + Latru, 15min
Cyto + ATP + GTPγS, 5min
Cyto + ATP + GTPγS, 15min
Number of the ccp
Center Height of Individual Clathrin Coated Pits (nm)

5. Supplemental Figure-5 De Camilli
FBP17
Actin b
FBP17
Intensity (a.u.)
+ Latru
+ Latru
+ Latru
Actin c
Before t = 0 sec sec sec sec sec sec
FBP17
PM-GFP d
PM-GFP
Intensity (a.u.)
FBP17

6. Supplemental Figure-6 De Camilli
Protein A Sepharose Beads
+anti-GFP antibody
Protein A Sepharose Beads
PM-GFP