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Effects of liver depression and psychological stress on human uterine leiomyoma cells by an AR–cAMP–PKA signal transduction pathway Tian Xia, Shuang Li, Ruihong Ma, Sufen Guan, Jiacui Li, Hongqin Li, Hexin Zhang, Qiu Lin, Zhimei Zhao, Baojuan Wang Taiwanese Journal of Obstetrics and Gynecology Volume 56, Issue 3, Pages (June 2017) DOI: /j.tjog Copyright © Terms and Conditions
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Fig. 1 The growing leiomyoma cells (HE staining, Magnification ×100).
Taiwanese Journal of Obstetrics and Gynecology , DOI: ( /j.tjog ) Copyright © Terms and Conditions
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Fig. 2 The growing leiomyoma cells (Immunohistochemistry, Magnification ×100) (staining for alpha-smooth muscle actin). Taiwanese Journal of Obstetrics and Gynecology , DOI: ( /j.tjog ) Copyright © Terms and Conditions
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Fig. 3 The MTT mean values in different dose groups and measurement time-points are shown. Time and dose factors significantly affected cell proliferation as determined by the MTT assay in human uterine leiomyoma cells. By multiple analyses of different doses in the NE and control groups (A), the data suggested that OD values of different dose groups were higher than that found for the control group (P = 0.001). The OD value was highest in the 1 μM group, and was highest at 12 h than was found for 3 h, 6 h and 24 h (P = 0.0001, P = 0.0001, and P = 0.029 respectively). B. shows that the OD value in each dose of the E groups was higher than the control group (P = 0.028, P = 0.0001, and P = 0.0001), and the 1 μM group at 12 h was the highest. In Fig. C, the OD value for 1 μM and 10 μM for the ISO group was lower than the blank group (P = 0.001, and P = 0.0001), and at 24 h the OD value was the lowest. Taiwanese Journal of Obstetrics and Gynecology , DOI: ( /j.tjog ) Copyright © Terms and Conditions
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Fig. 4 Graphs demonstrating the mean VEGF and FGF protein expression values of different dose and time-point groups. The VEGF and FGF protein expression by ULM cells was statistically significant in terms of both dose and time-dependent factors. Taiwanese Journal of Obstetrics and Gynecology , DOI: ( /j.tjog ) Copyright © Terms and Conditions
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Fig. 5 Graphs demonstrating the mean ER and PR protein expression values of different dose and time-point groups. Taiwanese Journal of Obstetrics and Gynecology , DOI: ( /j.tjog ) Copyright © Terms and Conditions
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Fig. 6 Graphs demonstrating representative results from seven separate studies in uterine leiomyoma cells. The incubation was interrupted after 12 h of treatment. The data was represented as mean value ± SD. The differences between the drugs and controls were also significant (P < 0.05). Taiwanese Journal of Obstetrics and Gynecology , DOI: ( /j.tjog ) Copyright © Terms and Conditions
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Fig. 7 Western blot analysis of PKA in uterine leiomyoma cells with NE and NE plus phentolamine (NE+). Taiwanese Journal of Obstetrics and Gynecology , DOI: ( /j.tjog ) Copyright © Terms and Conditions
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Fig. 8 Graphs demonstrating representative results from seven separate studies in uterine leiomyoma cells. The incubation was interrupted after 24 h. The data was represented as mean value ± SD. The differences between the drugs and controls were significant (P < 0.05). Taiwanese Journal of Obstetrics and Gynecology , DOI: ( /j.tjog ) Copyright © Terms and Conditions
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Fig. 9 Western blot analysis of PKA in uterine leiomyoma cells with ISO and ISO plus metoprolol (ISO+). Taiwanese Journal of Obstetrics and Gynecology , DOI: ( /j.tjog ) Copyright © Terms and Conditions
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