1. Olive Oil Polyphenol Oleuropein Inhibits Smooth Muscle Cell Proliferation 
R. Abe, J. Beckett, R. Abe, A. Nixon, A. Rochier, N. Yamashita, B. Sumpio 
European Journal of Vascular and Endovascular Surgery 
Volume 41, Issue 6, Pages (June 2011)
DOI: /j.ejvs
Copyright © 2010 European Society for Vascular Surgery Terms and Conditions

2. Figure 1
Growth inhibition induced by oleuropein. SMC were exposed to 100 μM of oleuropein for 5 days or SMC were exposed to 100 μM of oleuropein for one day and then grown without oleuropein to determine reversibility. A) Cell growth curve for a typical experiment. The x-axis represents the time course of the experiment, and the y-axis represents the cell number per well. ∗p < 0.05 compared with control on day 5. There was significant inhibition (by 92%) of SMC growth in the presence of oleuropein. Experiments were repeated in triplicate. B) Light power microscopy for SMC with and without oleuropein × 100 magnification. C) Oleuropein was removed on day1. Cell growth inhibition could be reversed by removing the polyphenol.
European Journal of Vascular and Endovascular Surgery  , DOI: ( /j.ejvs )
Copyright © 2010 European Society for Vascular Surgery Terms and Conditions

3. Figure 2
A) Representative flow cytometric histograms of SMC in the absence or presence of oleuropein for 1 or 5 days. The y-axis in histograms represents the number of cells which are in the G0-1 (growth, first curve), S (DNA synthesis, second curve), or G2–M (mitosis, third curve), phase of the cell cycle. Experiments were repeated in duplicate. B) Bar graph representation of percent of SMC in various phases of the cell cycle. ∗p < 0.05 compared with control by Pearson’s chi-square test.
European Journal of Vascular and Endovascular Surgery  , DOI: ( /j.ejvs )
Copyright © 2010 European Society for Vascular Surgery Terms and Conditions

4. Figure 3
Immunoblot for phospho/total-ERK1/2, phospho/total-Rb, cyclinD, p27, and p21 of SMC in the absence or presence of 100 μM oleuropein. C: control, O: oleuropein-treated group.
European Journal of Vascular and Endovascular Surgery  , DOI: ( /j.ejvs )
Copyright © 2010 European Society for Vascular Surgery Terms and Conditions

5. Figure 4
Growth inhibition induced by PD SMCs were exposed to 100 μM of PD98059 for 5 days or SMCs were exposed to 100 μM of PD98059 for one day and then grown without PD The x-axis represents the time course of the experiment, and the y-axis represents the cell number per well. ∗p < 0.05 compared with control on day 5. Experiments performed in duplicated. A) Cell growth curve for a typical experiment. B) PD98059 was removed on day 1. Cell growth inhibition could be reversed by removing the PD98059.
European Journal of Vascular and Endovascular Surgery  , DOI: ( /j.ejvs )
Copyright © 2010 European Society for Vascular Surgery Terms and Conditions