1. CEBZB Department of Carbohydrate Enzymology Head: Jozef Nahálka, PhD
Slovak Academy of Sciences, Institute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Dúbravská cesta 9, SK Bratislava, Slovak Republic
CEBZB
Department of Carbohydrate Enzymology
Head: Jozef Nahálka, PhD
Laboratory of Synthetic Biology: Nahálka J., Hrabárová E., Talafová K.
Laboratory of microbial degradation of plant cell walls: Biely P., Vršanská M., Puchart V., Šuchová K., Fraňová, L.
selected paper
Nahalka, J., Hrabarova, E., & Talafova, K. (2015). Protein-RNA and protein-glycan recognitions in light of amino acid codes. Biochimica Et Biophysica Acta - General Subjects, 1850(9), doi: /j.bbagen
Biely, P., Sing, S., & Puchart, V. (2016). Towards enzymatic breakdown of complex plant xylan structures: State of the art. Biotechnology Advances, 34(7), doi: /j.biotechadv

2. CEBZB Ruegeria pomeroyi PPK2 PPK1 PPK3
Slovak Academy of Sciences, Institute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Laboratory of Synthetic Biology, Dúbravská cesta 9, SK Bratislava, Slovak Republic
CEBZB
PPK1
PPK2
PPK3
Ruegeria pomeroyi
Industrial biocatalysis – is limited to “energy-independent enzymes” such as hydrolases, which perform relatively simple chemistry. Living cells in nature – are capable of using much more complex chemistry by cofactor-dependent enzymes, especially by nucleoside triphosphate (NTP) ‘‘burning’’ enzymes. We study polyphosphate kinases (PPKs) for NTP regeneration.

3. Slovak Academy of Sciences, Institute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Laboratory of Synthetic Biology, Dúbravská cesta 9, SK Bratislava, Slovak Republic
CEBZB
Glycosyltransferases – There is no doubt that the oligosaccharides can be used in the prevention, diagnosis and in treatment of chronic diseases. We study glycosyltransferases required for oligosacharide synthesis.

4. Hypothetical Plant Xylan and Enzymes Required for Its Hydrolysis
Slovak Academy of Sciences, Institute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Laboratory of Microbial Degradation of Plant Cell Walls, Dúbravská cesta 9, SK Bratislava, Slovak Republic
CEBZB
Hypothetical Plant Xylan and Enzymes Required for Its Hydrolysis
PhA (Fe, p-Coum) 
Araf Araf
Ac  1
  
    ( )   () 3
Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl1-4Xyl-4Xyl1-
  
 Ac 1
MeGlcA MeGlcA
  
Lignin alcohol
 endo--1,4-xylanase (EC ) 
 -xylosidase (EC ) or exo--xylanase Xyl1-4Xyl1-
 -glucuronidase (EC )
 -L-arabinofuranosidase (EC )
 feruloyl esterase or p-coumaroyl esterase (EC )
   acetylxylan esterase (EC )
 glucuronoyl esterase (EC )

5. Recent findings of the laboratory
Slovak Academy of Sciences, Institute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Laboratory of Microbial Degradation of Plant Cell Walls, Dúbravská cesta 9, SK Bratislava, Slovak Republic
CEBZB
Recent findings of the laboratory
Unique combination of catalytic properties of GH30 xylanase IV from Trichoderma reesei – weak endo-action on polymers and release of reducing-end xylose residue from linear oligosaccharides (2013)
Novel eukarytic GH30 glucuronoxylanase (Trichoderma reesei XYN VI) resembling bacterial counterparts (2014)
Migration of acetyl group along xylopyranoside ring (2015)
Complementarity in regioselectivity of CE1-CE7 acetylxylan esterases and CE16 acetyl esterases ( )
CE16 enzymes are non-reducing-end exo-deacetylases but som show a weak acetylxylan esterase activity ( )
Novel chromogenic substrates for detection and quantification of glucuronoyl esterase activity (2016) using a b-glucuronidase-coupled assay