1. Who ate the Cheese??
Forensic Science 12/3/14

2. Drill – Pd. 3, 5
Put these steps for RFLP analysis in order. What is each step for?
Look at film and analyze
Use gel electrophoresis
Radioactive probes
Southern Blotting
Treat DNA with restriction enzymes
X-ray film
HW: Finish Paternity Testing or DNA in the Courtroom, if needed

3. Drill – Pd. 6 Which man is the father?
4th page of packet, on the front
Answer questions
HW:
The Break-in (back of 4th page)
Finish Paternity Testing or DNA in the Courtroom, if needed

4. Objectives IWBAT Analyze DNA to determine the most likely suspect.
Analyze a RFLP test.

5. Agenda Drill Who ate the Cheese? lab
Paternity Testing or DNA in the Courtroom
Closure

6. Who Ate The Cheese?
Oh no! Someone ate the Queen’s prize Limburger cheese!
Saliva samples were taken from the teeth marks left on the cheese
How can we determine who ate the cheese?

7. DNA Fingerprinting
A process which uses fragments of DNA to identify the unique genetic makeup of an individual.
PCR
Restriction Enzymes
Electrophoresis

8. PCR Polymerase Chain Reaction Replicates DNA sample
Produces millions of copies in 2 hours

9. Restriction Enzymes
Molecules that cut DNA segments into smaller pieces by breaking bonds
Cuts DNA into fragments of different lengths

10. Gel Electrophoresis GEL ELECTROPHORESIS
DNA molecules have a negative charge.
The molecules migrate toward the positive pole.
The larger the molecule, the more difficult it is to make its way through the gel, so the shorter segments move farther than longer ones.

14. Who Ate the Cheese? Work in pairs. Ms. Bloedorn will assign the pairs.
Each pair will get:
A description of the case scenario (Class set—don’t take!!)
DNA sheet (you will cut this up)
Analysis sheet (you will be turning this in)
A sheet of printer or graph paper (your “gel”)
A roll of tape—not enough for pairs, share with your table.
If not done at the end of class, put it in your folder until tomorrow.

15. Steps to Who Ate the Cheese?
Read the scenario and the directions.
One partner will prepare the “samples” (Steps 1&2)
One partner will prepare the “agarose gel” (Step 3)
Create the DNA “gel” together.
Create your finished “gel” and answer the questions on the back of the scenario paper.
Staple the “gel” and questions together. Turn in the final product.

16. Who Ate The Cheese?
After cutting out your fragments, count how many base pairs there are in each fragment. You may color-code the fragments, if you like (ex. Suspect 1 = blue, etc.)
Lay it out horizontally, as Ms. Bloedorn demonstrates. Your range is 0-31 bps.
You may end up with fragments overlapping on “lanes”. Just fold the fragments over before taping them down.

18. Choose a Case! You get to decide: Paternity Testing
DNA in the Courtroom
Hold onto this for discussion!

19. Closure How many bands do you expect for each RFLP tested? Why?
Who discovered the structure of DNA?
Which proteins help unwind DNA during replication?