1. From: Corneal Sulfated Glycosaminoglycans and Their Effects on Trigeminal Nerve Growth Cone Behavior In Vitro: Roles for ECM in Cornea Innervation
Invest. Ophthalmol. Vis. Sci ;53(13): doi: /iovs
Figure Legend:
Trigeminal neurite behavior following elongation upon intermediate, permissive concentrations of immobilized GAG substrates. (A–E) The strategy for generating culture substrates so that trigeminal neurons grow out on surfaces containing PDL/laminin and GAG or control spots was described in Figure 5A. A vertical line above (A–C) or below (D, E) the image indicates the location of the boundary between the PDL/laminin only substrate (control, left) and the test substrate (GAG or control spot, right). Representative images are shown for neurites encountering a water control spot (A) or CSA (B), DS (C), CSC (D), or KS (E). Neurite behavior upon growing over CSA (B), DS (C), and CSC (D) is characterized by a marked shift from intermediate to thick fascicles (white arrows, fasciculation) and reduced defasciculation/branching. On the contrary, neurite behavior seen when growth cones migrate upon KS (E) is characterized as nonfasciculated (yellow arrows, nonfasciculated) with a high degree of branching (white arrowheads represent points of branching or defasciculation). (F) Percentages of neurites that grew as fasciculated or nonfasciculated under control and test conditions. (G) Percentages of branching points per neurite under control and test conditions. n values: water, 89 neurites; CSA, 127 neurites; DS, 114 neurites; CSC, 110 neurites; KS, 174 neurites.
Date of download: 10/13/2017
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