1. ELISA
By Codey & Marquell

2. ELISA
What is ELISA
ELISA stands for: Enzyme-linked Immunesorbant Assay.
A way to sample large quantities of proteins and antibodies.

3. What does ELISA do?
The point of the ELSIA is to find out if a certain type of protein is in the sample or how much of the protein is in it.

4. How can it be used to Identify Bt Corn?
ELISA can find a certain protein or antibody in a test sample.

5. Direct Vs. Indirect ELISA- Direct
Advantages
Disadvantages
Its quicker because only one antibody is found
Fewer steps are used
Cross-reactivity of secondary antibody is eliminated
Immune-reactivity might be changed do to labeling with enzymes or tags.
Labeling primary antibodies for each system is time-consuming and expensive
No choice of primary antibody label from one experiment to another.

6. Continued… Indirect Advantages Disadvantages
A large amount of labeled secondary antibodies are for sale.
Maximum immune-reactivity of the primary antibody is retained because it is not labeled.
Different markers can be used with the same primary antibody.
Cross-reactivity might occur with the secondary antibody.
An extra incubation step is required in the procedure.

7. What it can Detect.
Proteins
Antibodies

8. Sources
Rapidtest.com/blog/tag/what-is-elisa-test
Piercenet.com