1. The Human Microbiome Project
Giada Cattelan Master in Advanced Genetics Genomics
The Human Microbiome Project
A comparison between HGP and HMP

2. Summary INTRODUCTION Microbioma Where is it? Metagenomics
THE HUMAN MICROBIOME PROJECT
Differences between HGM and HMP
Methods
Results
Goals
CONCLUSION
Summary

3. Which is the correlation?
The Human Microbioma
We are not alone
Which is the correlation?

4. Microbioma 10x more bacteria than cells
Digestion, Immune System, Protection, Metabolism,…
In every part of the body
Bacteria, Virus, Archea, Fungi
Microbioma

5. Where? Oral cavity Attached keratinized gingiva (gums)
Buccal mucosa (cheek)
Hard palate
Palatine tonsils
Saliva
Subgingival plaque
Supragingival plaque
Throat
Tongue dorsum
Nasal cavity
Anterior nares (nostrils)
Skin
Left and right antecubital fossa (inner elbow)
Left and right retroauricular crease (behind the ear)
Gastrointestinal tract
Stool
Urogenital tract
Mid vagina
Posterior fornix
Vaginal introitus
Where?

6. Metagenomics is the study of genetic material recovered directly from environmental samples.
Advantage from the new techniques
Metagenomics

7. The Human Microbiome Project
PRINCIPAL AIM:
Characterization of the human microbiome from multiple body site.
Production of a reference dataset as baseline
Develop a catalog of microbial genome sequences of reference strains
Determine changes in the microbiome and health/disease -
Large-genome-scale community research projects
Continuation of the HGM
The Human Microbiome Project

8. New Protocols and Methods
Privacy, Information
Sequen-cing
Applica-bility
Software
Managment of data
Goals

9. Differences between HGM and HMP
HGP
Published in (finished in 2004)
2 Projects: published and private
Competition
Hierarchical Shotgun and WGS
HMP
Not finished
1 project with many collaborations
Open to everybody
16S sequencing and WGS protocols
Differences between HGM and HMP

10. Results Sequenced: 1900 microbial strains
Aspectative: 3000 microbial strains
First 178 samples:
predicted genes
novel genes
600 strains already avaiable
Results

11. Results

12. Conclusion Community feedback for new strains «Most wanted Taxa»
Inform the medical science about the changes in the microbiome in diseases
Reference for the future
Conclusion

13. THE END

14. The NIH HMP Working Group, “The NIH Human Microbiome Project”, Published by Cold Spring Harbor Laboratory Press on September 24, 2009.
The Human Microbiome Project Consortium, “A framework for human microbiome research”, Published on Nautre, on 14 June 2012
L.M.Proxtor, “The Human Microbiome Project in 2011 and beyond”, published by Cell Host and Microbe on 20 October 2011
Official Wbsite Human Microbiome Project:
Literature