1. INTRODUCTION RESULTS&DISCUSSION OBJECTIVES METHODOLOGY FUTURE WORK
Synthesis and Characterization of Bioactive Composite for Dental Application
Zenab Yaasir*, A.S Khan*
*Interdisciplinary Research Centre in Biomedical Materials, COMSATS Institute of Information Technology, Lahore, Pakistan
INTRODUCTION
RESULTS&DISCUSSION
Endodontic therapy (Root Canal Treatment) is the
process of removing infected pulp from within the
roots of teeth and finally shaping, filling and
sealing the root canal system .
The use of bioactive, tooth-like material can be
employed as a good option for filling and sealing
the root canal system with added advantage of
endodontic tissue regeneration.
FTIR ANALYSIS :
Characteristic absorption bands :
Pure TCP powder:
Pronounced at 1040 cm-1(PO3-4)& 3756 cm-1 (H-O-H)
B. TCP-Gelatine cross linked composites:
I) Additional absorption bands at 1532cm-1&1536cm-1
owing to N-H stretching of (N-H-R) group.
(II) Absorption bands were also observed at 1659 cm-1
due to stretch mode of C≡N.
(III) At 1462 cm-1, presence of C=O corresponded to
residual/un mixed cross linking agent.
(IV) Additional band at cm-1 was due to
stretching mode of N-H mode in gelatin.
THERMOGRAVIMETRIC ANALYSIS:
Temperature range : 230 to 800 C
Heat ramp : 10 C/min
Sample weight decreased with increasing
temperature with an average of approximately 85%
weight loss.
Therefore thermal stability at oral temperature (37 C)
SCANNING ELECTRON MICROSOPY:
Uniform distribution of powder particles within composite
1040 :PO3-4
3756 : H-O-H
OBJECTIVES
The purpose of this study was to develop a bioactive
composite with potential application for endodontic
therapy. (Root canal treatment)
METHODOLOGY
Local Regeneration of Dentin-Pulp Complex
The experimental Bioactive composite composed of :
TCP:
Tricalcium Phosphate
GELATIN:
SAMPLE PREPARATION
TCP powder was synthesized by microwave method
with a Ca:P ratio of 1.5.
It was incorporated into commercial gelatin on
dissolution in water at specific temperature and
cross linking agent was added.
After homogenous stirring, the composite was
freeze dried to achieve TCP-Gelatin composite films.
Five percentage ratios were prepared of TCP:Gelatin
composite and all Five groups further characterized:
CHARACTERIZATION:
Fourier Transform Infra Red spectroscopy (FTIR)
Thermogravimetric Analysis(TGA)
Scanning Electron Microscopy (SEM
1532&1536: N-H stretch
1462: C=O
1659: C≡N stretch
: N-H stretch
Schematic diagram of Root Canal Treatment
Periapical radiograph of infected and
root canal treated tooth
FUTURE WORK
Includes further characterization:
Raman Spectroscopy.
XRD
Physico-mechanical testing:
Compressive strength
Hardness
The future work would encompass:
Biocompatibility/cell toxicity studies
Anti microbial testing
In vitro cell culture studies
Animal trials and Clinical trials
CONCLUSION
REFERENCES
These analyses would assist in justifying the use of
TCP-Gelatin composites in various areas of dentistry
The main emphasis being on endodontic/root canal
therapy, owing to their possible osteoconductive and
antimicrobial properties.
ACKNOWLEDGEMENTS
R.W. Bucholz, A. Carlton and R.E. Holmes, Hydroxyapatite and tricalcium phosphate
bone graft substitutes, Orthop. Clin. North Am., I8 (1987) 323
Kitamura C, Nishihara T, Terashita M, Tabata Y, Washio A - Int J Dent (2011)
Adams AM, Soames JV, Searle RF. Morphological changes in cultured human
periodontal ligament cells exposed to dental materials: Biomaterials (1995)16:
Comsats Institute of Information Technology
Pakistan Science Foundation
Higher Education Commission