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Material for Introductory Microbiology

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Presentation on theme: "Material for Introductory Microbiology"— Presentation transcript:

1 Material for Introductory Microbiology
Computer Filename: Protsynb.ppt Protein Synthesis Material for Introductory Microbiology

2 Overview Genes are composed of DNA within the chromosome
Genes code for proteins DNA is transcripted into RNA RNA is translated into protein by ribosomes Genes regulated by control of RNA production

3 Lecture Outline Protein synthesis tRNA structure
adding amino acids to tRNA ribosome structure ribosome attachment protein synthesis :initiation, elongation, attachment

4 Concepts from OAC Structure & Function of DNA & RNA Genetic code
Transcription & translation Protein synthesis

5 Background Terms & Concepts
Structure of DNA & RNA Ribosomes Enzymes

6 CODONS sequences of three adjacent nucleotides that code for one amino acid or polypetide termination 64 codons encrypt of ~ 20 amino acids degenerate ACU, ACC,ACA, ACG threonine Start codon AUG (also codes for methionine) Stop codon UAA,UAG,UGA (nonsense codons)

7 Interpreting the Genetic Code
Two step process :Transcription -translation Transcription DNA --> RNA Translation RNA--> protein

8 Transcription Production of an RNA copy of the gene occurs in nucleus
DNA unravels- single strand RNA Polymerase binds to Promotor site Reads to stop codon assembles complementary single strand of RNA (primary RNA) Disengages when reaches stop codon

9 Protein Synthesis Formation of an initiation complex Chain elongation Chain termination

10 Concept Map: Protein Synthesis

11 Initiation of Protein Synthesis

12 Peptide Elongation

13 tRNA-AA Binding

14 Protein Membrane Signal Sequences

15 Cytoplasmic signal sequences

16 Transmembrane Sequences

17 EM of ER

18 Golgi Signalling

19 Overview of Protein Synthesis

20 Transcription-translation Overview


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