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Immunologic features of infants with milk or egg allergy enrolled in an observational study (Consortium of Food Allergy Research) of food allergy Scott H. Sicherer, MD, Robert A. Wood, MD, Donald Stablein, PhD, A. Wesley Burks, MD, Andrew H. Liu, MD, Stacie M. Jones, MD, David M. Fleischer, MD, Donald Y.M. Leung, MD, PhD, Alexander Grishin, PhD, Lloyd Mayer, MD, Wayne Shreffler, MD, PhD, Robert Lindblad, MD, Hugh A. Sampson, MD Journal of Allergy and Clinical Immunology Volume 125, Issue 5, Pages e8 (May 2010) DOI: /j.jaci Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions
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Fig 1 Real-time RT-PCR results by stimulant comparing children with confirmed/convincing milk or egg allergy (A) or sensitization status to milk, egg, or peanut (B). Values shown are median ΔΔCt values, as described in the text. Negative values indicate increasing expression. The number of infants in each category is shown alongside the ΔΔCt value. CISH, Cytokine-inducible SH2-containing protein; FOXP3, forkhead box protein 3. Journal of Allergy and Clinical Immunology , e8DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions
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Relationship of skin test wheal sizes (in millimeters) to serum IgE antibody levels (in kilounits of antibody per liter) for milk (A), egg (B), and peanut (C) shown on a logarithmic scale. Spearman correlation coefficients are 0.64 for milk and 0.65 for egg and peanut. +, Those with confirmed/convincing milk/egg allergy; Spearman correlation coefficients are 0.47 for milk and 0.48 for egg (P < .001 for this subgroup). SPT responses were recorded as negative if the saline control wheal was larger than the food test wheal. Journal of Allergy and Clinical Immunology , e8DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions
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Reproducibility of replicate PCR: correlation of 2 housekeeping gene's threshold cycle (CT) numbers from 2,708 PCR assays using 5 stimulation conditions. Spearman correlation coefficient is 0.84. Journal of Allergy and Clinical Immunology , e8DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions
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Reproducibility across study sites: the 5 participating sites (DC, National Jewish Health, Denver, Colo; DU, Duke, Durham, NC; JH, Johns Hopkins, Baltimore, Md; MS, Mount Sinai, New York, NY; and UA, University of Arkansas, Little Rock, Ark) prepared stimulated lymphocytes that were shipped to a central laboratory for quantitative PCR analysis. The site-specific results for the peanut-stimulated IL4 gene ΔΔCt are shown (n ranges from per site; there was no significant difference between sites for the detection of IL4, P = .32). Journal of Allergy and Clinical Immunology , e8DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions
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