1. Chromatography
chromatography

2. What is chromatography?
It’s a technique used to separate the components in a mixture

3. Why do we use chromatography?
We can use chromatography to separate the components of inks and dyes, such as those found in pens, markers, clothing, and even candy shells.

4. Chromatography can be used to separate the colored pigments in plants

5. It can also be used to see if certain species are closely related to each other by using samples of their DNA

6. How is it done? You place a mixture on a piece of chromatography paper
Place the end of the paper in a solvent

7. important rule
The line of dye on the strip must NOT touch the solvent

8. The components on the paper will start to travel up the paper
The colors of the components will separate and form a banding pattern of colors

9. The components of the mixture that are more soluble {dissolves better} will travel faster and farther up the paper
The color at the top of your banding pattern on your chromatogram is the color that was more soluble

10. Examples of Chromatography
Liquid Chromatography
Used to identify unknown plant pigments & other compounds.
Paper Chromatography
Can be used to separate the components of inks, dyes, plant compounds (chlorophyll), make-up, and many other substances
Thin-Layer Chromatography
Uses thin plastic or glass trays to identify the composition of pigments, chemicals, and other unknown substances.
Gas Chromatography
Used to determine the chemical composition of unknown substances, such as the different compounds in gasoline shown by each separate peak in the graph below.