1. Advanced Light Microscopy Core
BioFrontiers
Advanced Light Microscopy Core
Joe Dragavon

2. Advanced Light Microscopy Core
Our Mission:
To develop and apply cutting-edge versatile, reconfigurable, high-speed and high-sensitivity imaging technologies that aid and assist the observation of dynamic biological and physical processes in situ, in vitro, and in vivo.

3. Imaging Core: Guiding Foundation
Advanced Light Microscopy Core
Imaging Core: Guiding Foundation
Service
Imaging
Center
Independent
Research
Collaborations

4. Imaging Core: Responsive Network
Advanced Light Microscopy Core
Imaging Core: Responsive Network
The Imaging Center must be dynamic and flexible;
Rigidity = Stagnant
Synergy is key
Service
Increased Knowledge
Financial Independence
Commercially Available
Technologies
Imaging
Center
Independent
Research
Collaborations
Technology/
Probe/Method
Development

5. Key Implementation: Established Workflow
Results from an up to date infrastructure and ideological alignment
Sample Preparation
Acquisition
Image Treatment
Image Analysis
Quantification
Result Validation
Data
Storage
Signal Enhancement
Feature Extraction
Measures & Classification
Statistics & Interpretation
Multi modal, Multi dimensional, and Multivariate

6. Imaging Core: Staff Organogram
Advanced Light Microscopy Core
Imaging Core: Staff Organogram
Steering Committee
Joe Dragavon
Director
Amy Palmer
Faculty Advisor
User Committee
Joe Dragavon
Outreach
Maintenance
Administration
Development
Trainings

7. Core: Available Technologies
Advanced Light Microscopy Core
Core: Available Technologies
Widefield
Olympus IX-81
Nikon TE-2000
Zeiss Axiovert 200M
Analysis Workstation
Intel Quad Cor i7
Windows 64-bit
32 GB RAM
eForce GTX 690 graphics card
enables GPU processing
Software:
Imaris
Matlab
ICY
Image J/Fiji
Cell Profiler
Nikon Elements Analysis
Confocal
Nikon A1R Laser Scanning and TIRF
Nikon TiE Spinning Disc Confocal (August 2015)
High-Throughput
Molecular Devices ImageXpress
Super Resolution
Nikon N-STORM and TIRF
In the pipeline
Palmer/Piestun MRI: Multidimensional microscope
DoubleHelix 3D Super Resolution module
Important: Coordinating with Jolien Tyler
Also available: Portable environmental chamber with ˚C, Humidity, O2, and CO2 control

8. Here to help with your imaging needs!
Advanced Light Microscopy Core
Here to help with your imaging needs!
Thank you