1. Conclusions and perspectives
Designing an environmental cell for the adhesion characterization by force spectroscopy
B. Manship1,2,3, F. Ferry1,2,3, C. Roux4,5, M. Terrien1,2,3, C. Sillard1,2,3,F. Dubreuil4,5, R. Passas1,2,3
Introduction
Specifications
Papers are built on cellulosic fibers which are hygroscopic material. The force of adhesion and the work of adhesion between two cellulosic oligomers are depending on their level of humidity. To manage force spectroscopy by AFM under controlled humidity, a setup has to be developed.
An environmental chamber was built and could be mounted on the AFM platform. Experiments at varying humidity can be performed through the use of an air generator.
Air flow:
- Processing temperature = room temperature
- Relative Humidity regulation : 20% – 80%
- Flow rate control to avoid tip vibration
Environmental cell:
- Height => compatible with the AFM configuration
- Air flow circulation => no induced movement of the sample
Other:
- Transportable system
Air generator
Environmental cell
Setup
Humidity generation unit
PC unit
Outlet
Sample
Optical camera
AFM piezo head
Evaporation skirt
Air flow
inlet
Liquid cell
Humidity measurement
Flow rate measurement
Air flow circulation
Inlet
Conclusions and perspectives
The setup is now operational.
First results shows that there is no influence of the air flow on the spring constant of the AFM’s tip.
The impact of the flow rate on the adhesion force and on the work of adhesion is under evaluation.
Acknowledgements
This work was conducted within the CellAdher project supported by the PolyNat Carnot Institute
1Univ. Grenoble Alpes, LGP2, F Grenoble, France 2CNRS, LGP2, F Grenoble, France
3Agefpi, LGP2, F Grenoble, France
4Univ. Grenoble Alpes, CERMAV, F Grenoble, France 5CNRS, CERMAV, F Grenoble, France