1. * * * * B A WT RORa hypo C WT RORa hypo D RORa hypo RORa hypo
WAT
Liver
Brain
Relative RORa expression B
Relative RORa expression * * * C
mg/dL WT
RORa hypo D
% of cell population
% of cell population *
RORa hypo
RORa hypo
Supplemental Fig.1: RORa deficient mice show an anti-inflammatory profile:
A. RORa mRNA expression in white adipose tissue, liver and brain of males RORaWT (white bar, n=5) or RORaHypo (black bar, n=5). B. RORa mRNA expression in the intestine of males RORaWT (white bar, n=8) or RORaHypo (black bar, n=8) C. Weight, fat mass and lean mass of single housed 12 week-old males RORaWT (white bar, n=6) or RORaHypo (black bar, n=6)) littermate. C. CD4 (upper) or CD8 (lower) expression in lymph nodes freshly isolated from the 12 week-old male RORaWT (white, n=5) or RORaHypo (black, n=5) mice. *p<0,05.

2. Supplemental Fig.2: SR1001 does not impair macrophages polarization:
Relative expression
Supplemental Fig.2: SR1001 does not impair macrophages polarization:
Gene expression from BMDM treated with SR1001 (10uM) during M2 (IL-4 treated) or M1 5INFg/LPS treated) polarization. n=9

3. Body weight (g) A **
mg/dL B
% cell population
CD4+ cells
Vehicle
SR1001 D
U/L
% cell population * E
***
Supplemental Fig.3: SR1001 does not affect weight but decreases pro-inflammatory cells development in lymph nodes:
Body weight and blood lipid profile of single housed males LDL-R-/- treated with SR1001 (white bar, n=9) or vehicle (black bar, n=9) after 10 days of HC diet before 30 days dosing. B. CD4 cells population in lymph nodes freshly isolated from the same animals. C. Body weight of single housed males LDL-R-/- treated with SR1001 (white bar, n=9) or vehicle (black bar, n=9) after a month of HC diet during 30 days dosing and D. Plasma liver enzyme level after 30 days dosing. E. Treg, Th1, Th2 and Th17 cells population in lymph nodes freshly isolated from the same animals. (n=6). *p<0,05; ***p<0,001.

4. Anti-body
Clone
CD3
17A2
CD4
GK1.5
CD8a
53-6.7
CD115
AFS98
B220
RA3-6B2
IL-17R
9B10
Ly6G
1A8
CD25
PC61
Foxp3
MF-14
INFg
XMG1.2
Gata3
16E10A23
ABCG5
NBP
Supplemental Table1: List of anti-bodies used
All the flow cytometry anti-bodies come from Biolegend and ABCG5 come from Novus Biologicals