1. Narges Babadaei1, Hashem Nayeri1*, Gholamreza Amiri2
Study activity of LPO immobilized onto magnetic nanoparticles In presence of inhibitor: Compared with free enzyme for industrial function
Narges Babadaei1, Hashem Nayeri1*, Gholamreza Amiri2
Enzymes are the most skilful catalysts, offering much more competitive processes compared to chemical catalysts, they can catalyze a large number of reactions with high specificity and speed, under very mild environmental conditions and high selectivity.
To be used as practical biocatalysts, especially industrial biocatalysts, improvement of the activity, stability and recovery of enzymes are necessary. Enzyme immobilization provides a superior approach to achieve enzyme recovery, and establishes a better way to handle the enzyme and eliminate protein contamination of the product easily.
in this study reusable carrier for enzyme immobilization consisting of magnetic Fe3O4 nanoparticles coated with a layer of silica nanoparticles (SiO2NPs) and activity of Lactoperoxidase(LPO) immobilized on magnetic nanoparticles In presence of Cadmium chloride as an inhibitor has been demonstrated.
Introduction:
Results:(Continue)
Activity of free and immobilized enzyme in present of CdCl2
Materials & Methods:
Chart shows the number of Regain
nanoparticles were prepared by a simple in situ co-precipitation method after that coated by Silica and characterized by transmission electron microscope (TEM) and Fourier transform infrared spectroscopy (FTIR). After the synthesis of nanoparticles enzyme has stabilized on NPs surface. immobilized enzyme activity in the presence of Cadmium chloride(CdCl2) as an inhibitor has been studied.
immobilized enzyme activity in the presence of various concentrations of inhibitors was significantly more than free enzyme(P-value<.05).
These results showed that the immobilization of LPO onto NPs is an efficient and simple way for preparation of stable LPO for industrial function.
Discussion:
The immobilized enzyme
Synthesis of nanoparticles
Results:
References:
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TEM of Fe3O4 and NPs