1. From: A Period of Controlled Elevation of IOP (CEI) Produces the Specific Gene Expression Responses and Focal Injury Pattern of Experimental Rat Glaucoma
Invest. Ophthalmol. Vis. Sci ;57(15): doi: /iovs
Figure Legend:
Controlled elevation of IOP. (A) Custom-made head holder, anchoring the nose in the respiratory cone, with isoflurane delivery (green tube) and an active exhaust for waste gas and CO2 (red and white fitting). Balanced saline solution (BSS) tubing is anchored to the holder by two Silastic bands (arrows) that stabilize the needle, yet still allow delicate adjustment of the needle position in the eye. (B) Balanced salt solution PLUS delivered through the cornea using a modified, 31-gauge needle shortened to 1 cm with a modified bevel, the hub of which is fitted to flexible Silastic tubing (single arrow). Fixation of the animal's head and the tubing to the same holder minimizes chance of the needle becoming dislodged. (C) Intraocular pressure measured via a second anterior chamber needle (23-gauge) closely follows the intended pressure, delivered through a 31-gauge needle, when IOP is raised from 20 to 60 mm Hg, confirming that pressure measured in the 31-gauge needle line accurately reflects actual IOP.
Date of download: 10/14/2017
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