1. FIG. 3. SDS corneal viability measurement
FIG. 3. SDS corneal viability measurement. Isolated pig corneas were treated with SDS solutions at concentrations ranging from 3 to 30%. After incubation, resorufin fluorescence was read at 590 nm emission wavelengths with 570 nm excitation wavelengths. Results were expressed in fluorescence units (FU) and percentage of viability, calculated using the following equation: (FU treated/FU control) × 100. **p < 0.01; n = 12.
From: Resazurin Metabolism Assay Is a New Sensitive Alternative Test in Isolated Pig Cornea
Toxicol Sci. 2003;72(1): doi: /toxsci/kfg014
Toxicol Sci | © 2003 Society of Toxicology

2. FIG. 4. TX-100 corneal viability measurement
FIG. 4. TX-100 corneal viability measurement. Isolated pig corneas were treated with TX-100 solutions at concentrations ranging from 1 to 10%. After incubation, resorufin fluorescence was read at 590 nm emission wavelengths with 570 nm excitation wavelengths. Results were expressed in fluorescence units (FU) and percentage of viability, calculated using the following equation: (FU treated/FU control) × 100. *p < 0.05; n = 12.
From: Resazurin Metabolism Assay Is a New Sensitive Alternative Test in Isolated Pig Cornea
Toxicol Sci. 2003;72(1): doi: /toxsci/kfg014
Toxicol Sci | © 2003 Society of Toxicology

3. FIG. 5. Alcohols corneal viability measurement
FIG. 5. Alcohols corneal viability measurement. Isolated pig corneas were treated with six undiluted alcohols (propylene glycol, glycerol, ethanol, isopropanol, n-octanol, and n-butanol). After incubation, resorufin fluorescence was read at 590 nm emission wavelengths with 570 nm excitation wavelengths. Results were expressed in fluorescence units (FU) and percentage of viability, calculated using the following equation: (FU treated/FU control) × 100. **p < 0.01; n = 12.
From: Resazurin Metabolism Assay Is a New Sensitive Alternative Test in Isolated Pig Cornea
Toxicol Sci. 2003;72(1): doi: /toxsci/kfg014
Toxicol Sci | © 2003 Society of Toxicology

4. FIG. 6. Relation between percentage of corneal viability and maximum modified average score of the 15 tested irritants. The mathematical relation between MMAS and the percentage of corneal viability is represented by the following equation: y = –0.6174x
From: Resazurin Metabolism Assay Is a New Sensitive Alternative Test in Isolated Pig Cornea
Toxicol Sci. 2003;72(1): doi: /toxsci/kfg014
Toxicol Sci | © 2003 Society of Toxicology

5. FIG. 1. Resazurin and resorufin structures
FIG. 1. Resazurin and resorufin structures. The oxidized form corresponds to resazurin and the reduced form to resorufin.
From: Resazurin Metabolism Assay Is a New Sensitive Alternative Test in Isolated Pig Cornea
Toxicol Sci. 2003;72(1): doi: /toxsci/kfg014
Toxicol Sci | © 2003 Society of Toxicology

6. FIG. 2. BAC corneal viability measurement
FIG. 2. BAC corneal viability measurement. Isolated pig corneas were treated with BAC solutions at concentrations ranging from 1 to 10%. After incubation, resorufin fluorescence was read at 590 nm emission wavelengths with 570 nm excitation wavelengths. Results were expressed in fluorescence units (FU) and percentage of viability, calculated using the following equation: (FU treated/FU control) × 100. **p < 0.01; n = 12.
From: Resazurin Metabolism Assay Is a New Sensitive Alternative Test in Isolated Pig Cornea
Toxicol Sci. 2003;72(1): doi: /toxsci/kfg014
Toxicol Sci | © 2003 Society of Toxicology