1. Lecture 15 WEB: Go to pollev.com/ucibio TEXT: Text UCIBIO to 37607
After joining session, text your question

2. Lecture 15 objectives Understand role and reactions of Gluconeogenesis
Apply principles of regulation to Gluconeogenesis
Understand the Cori cycle
Understand signaling pathways that regulate Gluconeogenesis

3. Maintaining blood Glc levels
Glucose is the energy source for many tissues
Need to keep blood glucose level constant
Not taking in Glc  Blood glucose falls
How to put Glc into blood?

4. Carbon sources for Gluconeogeneis: Cori cycle

5. Carbon sources for Gluconeogeneis

6. Gluconeogenesis Gluconeogenesis Reverse of glycolysis
What step/s have to be different?

7. Cellular compartments
Where does Glycolysis occur?
Where does TCA occur?

8. Compartmentalization

9. 1: How to make PEP?
Cannot directly convert Pyruvate to PEP
So, make

10. 1: How to make PEP?
Metabolite compartmentalization must be overcome

11. 1: How to make PEP?

12. PEPCK and Superman?!??

13. 2: Reversing the irreversible reactions

14. 2: Reversing the irreversible reactions
Remember Fru 2,6-bis-PO4?

15. 3. Reversing the irreversible reactions

16. Reversing Glycolysis

17. Reversing Glycolysis

18. Avoiding “futile” cycles

19. Exceptions… Glycolysis Gluconeogenesis 25°C 7°C Rate
Fed animals; different temps
What is the BEST explanation for these results?

20. Exceptions… Condition when futile cycle might be useful?
Upload to “Futile cycle”
Midnight tonight

21. Signaling cascade recap
Example 2: Phosphofructokinase 2
LIVER
Adenylate cyclase
Low Glc ↑
cAMP
? PKA ?
Pancreas ?
? Fru 2,6-bisPO4
? PFK1; ? FBPase 1
? GLYCOLYSIS ? GLUCONEOGENESIS

22. Coordinating metabolism: Recap
Decreased Blood sugar
Make hormone: ___________________
Effect on liver: ___________________
Effect on muscle: ___________________
Effect on adipose tissue: ___________________
Increase Blood sugar
Make hormone: ___________________
Effect on liver: ___________________
Effect on muscle: ___________________
Effect on adipose tissue: ___________________