1. 243 Unit 3 Labs 1 and 4

2. Histology Slides for the Cardiovascular System
Slides are presented in order of magnification
As you view the following slides, make sure you can accomplish these goals:
Can you identify the vessels and tissue layers observable on the slides?
Can you identify the specific structures or layers indicated by the numbered arrows or brackets?
At the end of a sequence, you will find the answers to the above for each vessel and layer

3. 4 4 1 2 3 3
Overview (40X), Masson stain for connective tissue

4. Overview (100X), Masson stain for connective tissue4 3 6 9 7 10 5 8 1 2 3

5. 13 10 9 12 11 8
Vein or Artery? 400X

6. Vein or Artery? 400X 13 6 5 7 5 14

7. Artery, Vein, Nerve Tissue stained with Masson stain
Slides 2 through 5:
Artery
Vein
Nerve fascicles
Skeletal Muscle Tissue
Lumen of the vein (with RBC’s)
Vein Tunica Media- smooth muscle layer
Vein Tunica Externa- connective tissue layer
Lumen of the Artery (with RBC’s)
Artery Tunica Externa- connective tissue layer
Artery Tunica Media- smooth muscle layer

8. Artery, Vein, Nerve Tissue stained with Masson stain
Slides 2 through 5:
11. Artery Tunica Interna- endothelial cells (simple squamous)
Artery Tunica Interna- Internal elastic lamina
Capillary Tunica Interna- endothelial cells
Vein Tunica Interna- endothelial cells (simple squamous)

9. 3 4 3 2 2 2 1
Overview (40X), stained for elastic fibers

10. Vein or Artery? 400X 16 12 10 8 2 15 9

11. Vein or Artery? 400X 10 8 15 9 12

12. Vein or Artery? 400X 16 3 7 5 6 1

13. Vein or Artery? 400X 16 14 6 7 5

14. Artery, Vein, Nerve Tissue stained for Elastic Fibers
Slides 8 through 12:
Artery
Vein
Nerve fascicles
Skeletal Muscle Tissue
Lumen of the vein
Vein Tunica Media- smooth muscle layer
Vein Tunica Externa- connective tissue layer
Lumen of the Artery (with RBC’s)
Artery Tunica Externa- connective tissue layer
(demonstrating elastic fibers)
10. Artery Tunica Media- smooth muscle layer

15. Artery, Vein, Nerve Tissue stained for Elastic Fibers
Slides 8 through 12:
11. Artery Tunica Interna- endothelial cells (simple squamous)
Artery Tunica Interna- internal elastic lamina
Capillary Tunica Interna- endothelial cells
Vein Tunica Interna- endothelial cells (dark band is due to the elastic fibers in the basement membrane)
Artery Tunica Media- external elastic lamina
Adipose cells

16. Vein or Artery? 400X 16 7 6 5 14 8

17. Vein or Artery? 100X 2 16 8 9 10 12 3 3

18. 8 11 12 10 9
Vein or Artery? 400X

19. Artery, Vein, Nerve Tissue, Nonspecific Stain
Slides 15 through 16:
Artery
Vein
Nerve fascicles
Skeletal Muscle Tissue
Lumen of the vein
Vein Tunica Media- smooth muscle layer
Vein Tunica Externa- connective tissue layer
Lumen of the Artery (with RBC’s)
Artery Tunica Externa- connective tissue layer
(demonstrating elastic fibers)
10. Artery Tunica Media- smooth muscle layer

20. Artery, Vein, Nerve Tissue, Nonspecific Stain
Slides 15 through 16:
11. Artery Tunica Interna- endothelial cells (simple squamous)
Artery Tunica Interna- internal elastic lamina
Capillary Tunica Interna- endothelial cells
Vein Tunica Interna- endothelial cells (simple squamous)
Artery Tunica Media- external elastic lamina
Adipose cells

21. Specific Kind of Tissue? 400X3 1 2 2 2

22. Cardiac Muscle Tissue Slides 20: Nucleus of a Cardiac Muscle Cell
Intercalated Disc
Branching Fibers
Capillary Tunica Interna- endothelial cells

23. Identify Blood cells 3 2 1 2

24. Identify Blood cells 4 3

25. Identify Blood cells 2 4 3

26. Identify Blood cells 3 6 3 3

27. 7 3 5 5
Identify Blood cells

28. Identify Blood cells 7 2 3 3 3

29. Blood Cells Slides 22-27 Basophil Small Lymphocyte Neutrophil
Eosinophil
Monoctye
Large Lymphocyte
Platelets