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Frog Dissection
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Scientists believe other vertebrates evolved from BONY LOBE-FINNED fish
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Scientific American; Dec 2005; Vol 293; p100-107
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AMPHIBIAN CHARACTERISTICS
Moist, thin skin without scales Aquatic larva changes to terrestrial adult Feet without claws Respiration with gills, lungs, skin, mouth Closed 2 loop circulation Ectothermic (cold blooded) Eggs without shells or multicellular membranes
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FROG ANIMALIA CHORDATA VERTEBRATA “backbone” AMPHIBIA “double life”
LATIN meaning KINGDOM _____________ PHYLUM ____________________________ SUBPHYLUM ___________________________ CLASS _______________________________ ORDER _____________________________ ANIMALIA CHORDATA VERTEBRATA “backbone” AMPHIBIA “double life” ANURA “without a tail”
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Thin, moist skin – no scales
Mucous glands make it “slimy” Camouflage- for protection Some have poison glands
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ECTOTHERMIC “cold blooded”
Body temperature is dependent on surrounding environment
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HIBERNATION/ ESTIVATION
FAT stored in FAT BODIES provides energy
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Nictitating membrane
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NO CLAWS
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EXIT OPENINGS DIGESTIVE WASTE (feces) = _________________ ANUS
Shared EXCRETORY & REPRODUCTIVE EXIT = _____________________________ (Urine & eggs or Urine & sperm) ANUS UROGENITAL PORE
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Cloaca/Vent EXIT OPENINGS OPENING SHARED BY EXCRETORY, REPRODUCTIVE,
& DIGESTIVE = _____________ Cloaca/Vent
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What sex is it?
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AMPLEXUS “firm embrace”
Sperm and egg released at same time and place Increases chances of fertilization
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TONGUE attached at front not back like yours!
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Connect ears to back of throat
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GLOTTIS Opening to respiratory GULLET Opening to digestive
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Incisions for dissection
Place frog with ventral side up Make a longitudinal cut starting between the hind legs and going anteriorly to the tip of the jaw Make lateral cuts behind front legs and in front of hind legs First cut through the skin; repeat the incisions through the muscle layer
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Females may have black & white eggs that need to be removed to see the other organs.
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Pericardial membrane surrounds heart
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Mesentery holds intestines together and supports all organs.
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FAT BODIES Store fat for energy during Hibernation Estivation Breeding
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PYLORIC SPHINCTER CONTROLS passage of food from stomach into duodenum (intestine)
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Gall Bladder
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STOMACH: LIVER: GALL BLADDER:
Makes acid and digestive enzymes Starts digestion (grinds up food) Makes bile Stores glycogen Stores vitamins Processes toxins (including nitrogen wastes) for kidneys Stores bile
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Pancreas (enlarged)
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PANCREAS: Makes TRYPSIN, INSULIN, GLUCAGON
TRYPSIN- breaks down proteins INSULIN- tells cells to store glucose from bloodstream as glycogen GLUCAGON- tells cells to release stored glucose to blood stream
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Produces and stores new RBC’s and processes old worn out ones
SPLEEN Produces and stores new RBC’s and processes old worn out ones
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SMALL INTESTINE DUODENUM Receives trypsin and bile; finishes digestion
ILEUM Absorbs nutrients VILLI Increase surface area
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LARGE INTESTINE Removes water from digestive waste; concentrates feces
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Body Systems : ___________ __________ _____________ EXCRETORY AMMONIA
___________________ Gets rid of nitrogen waste made by cells Nitrogen waste has different chemical forms: ___________ __________ _____________ MOST TOXIC made from LEAST TOXIC ammonia by liver needs the least water to dilute FISH HUMANS BIRDS, REPTILES AMPHIBIANS EXCRETORY AMMONIA UREA URIC ACID
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ALL WASTE is NOT THE SAME!
DIGESTIVE waste- left over from undigested food travels through digestive system leaves through digestive system as feces EXCRETORY waste- (Also called NITROGEN WASTE) Made by cells from break down of proteins travels through blood stream Leaves through excretory system as Ammonia Urea uric acid (depending on type of animal)
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KIDNEYS- Remove nitrogen waste from blood and dilute it with water to make urine; osmoregulation
Image from:
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URINARY BLADDER STORES URINE MADE BY KIDNEYS LARVAE (Tadpoles)
Excrete AMMONIA like fish Adult frogs excrete UREA to conserve water
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CLOACA DIGESTIVE EXCRETORY REPRODUCTIVE
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LUNGS: GAS EXCHANGE
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BREATHING WITH LUNGS is called PULMONARY RESPIRATION
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Larvae exchange CO2 for O2 with GILLS
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OVARIES Make eggs
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Carries eggs to cloaca Adds jelly coating
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TESTES MAKE SPERM
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TESTES KIDNEY
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AMPLEXUS “firm embrace”
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INDIRECT DEVELOPMENT Grow legs; Lose tail 2 chambers 3 chambers
1 loop 2 loops Breathe w/ gills lungs & skin Excrete ammonia excrete urea (gills & kidneys) (kidneys)
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Ways tadpoles are like fish
Have a LATERAL LINE Breathe with gills Excrete nitrogen waste as AMMONIA (with gills & kidneys) Have a 2 chamber heart Have a 1 loop circulatory system
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HEART 3 chambered heart Right atrium Left atrium Ventricle
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TADPOLES & FISH: 2 chambered heart 1 loop system ADULT FROG:
Images from: TADPOLES & FISH: chambered heart 1 loop system ADULT FROG: 3 chamber heart 2 loop system
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MOST vertebrates have nuclei in their RBC’s
MAMMALS DON’T
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Fish/Tadpole circulation
Sinus venosus BODY organs Atrium GILLS Conus arteriosus Ventricle Fish/Tadpole circulation
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Conus arteriosus Frog circulation Sinus venosus Body organs
RIGHTAtrium LEFT Atrium Lungs Conus arteriosus Ventricle Frog circulation
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BRAIN
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