1. Products > L6 Transfection Reagent (Myeloma Cells, CRL-1458)
Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > L6 Transfection Reagent (Myeloma Cells, CRL-1458)
Altogen Biosystems offers the L6 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
L6 Transfection Reagent is an advanced formulation of a biodegradable polymer based transfection agent, and it is developed to provide high transfection efficiency with L6 and other mammalian fibroblast cell lines.
This cell line is a good host for studying mammalian tissue and connective tissue in particular.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase L6 Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. High Density L6 Cell Line
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > L6 Transfection Reagent (Myeloma Cells, CRL-1458)
L6 Cell Line Description and Applications
The L6 cell line originates from the fibroblasts of rat (Rattus norvegicus) thigh muscles and it is a particularly effective tool for studying connective and mammalian tissue. This cell line was isolated by Yaffe and passaged in methyl cholanthrene. The L6 cell line displays myoblast morphology and appears similar to skeletal muscle tissue. The cell line has several unique properties including the ability to fuse, the ability to create multinucleated myotubes and striated fibers as well as a propensity for high cellular variability. Due to the unique properties of L6, the cell line is frequently used in biological research studies.
High Density L6 Cell Line
Atcc.org
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. L6 Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > L6 Transfection Reagent (Myeloma Cells, CRL-1458)
L6 Transfection Protocol
Plate 10, ,000 L6 cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
Wash with 1xPBS and add 0.5 ml of fresh growth medium
Prepare transfection complexes by mixing 40 µl of serum free medium, 5.5 µl of transfection reagent, and • 750 ng DNA (or mRNA), or • 30 nM - 50 nM of siRNA (or microRNA) *Referred to a final volume including growth medium
Incubate transfection complexes at RT for minutes
Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity
Add prepared transfection complexes to 0.5 ml of complete growth medium with L6 cells (from step 2)
Incubate cells at 37ºC in a humidified CO2 incubator
Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of L6 cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > L6 Transfection Reagent (Myeloma Cells, CRL-1458)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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5. L6 Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > L6 Transfection Reagent (Myeloma Cells, CRL-1458)
L6 Transfection Kit Product Details
A biodegradable polymer based transfection agent – once inside the cell, the polymer degrades into smaller less toxic components reducing cell toxicity, facilitating release of the transgene, and improving transfection efficiency
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
High transfection efficacy in the presence of serum
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
Reproducible transfection results
Works well for standard reverse transfection and high-throughput applications
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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6. Data Products > L6 Transfection Reagent (Myeloma Cells, CRL-1458)
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > L6 Transfection Reagent (Myeloma Cells, CRL-1458)
Data
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the L6 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).

7. Data Products > L6 Transfection Reagent (Myeloma Cells, CRL-1458)
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > L6 Transfection Reagent (Myeloma Cells, CRL-1458)
Data
Figure 2. Protein expression of Cyclophilin B in L6 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into L6 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. L6 Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > L6 Transfection Reagent (Myeloma Cells, CRL-1458)
L6 Transfection Kit Benefits
Pre-optimized transfection protocol for L6 cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use L6 transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable L6 cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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